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Metaproteomics Reveals Abundant Transposase Expression in Mutualistic Endosymbionts
Transposases, enzymes that catalyze the movement of mobile genetic elements, are the most abundant genes in nature. While many bacteria encode an abundance of transposases in their genomes, the current paradigm is that the expression of transposase genes is tightly regulated and generally low due to...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Microbiology
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684830/ https://www.ncbi.nlm.nih.gov/pubmed/23781067 http://dx.doi.org/10.1128/mBio.00223-13 |
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author | Kleiner, Manuel Young, Jacque C. Shah, Manesh VerBerkmoes, Nathan C. Dubilier, Nicole |
author_facet | Kleiner, Manuel Young, Jacque C. Shah, Manesh VerBerkmoes, Nathan C. Dubilier, Nicole |
author_sort | Kleiner, Manuel |
collection | PubMed |
description | Transposases, enzymes that catalyze the movement of mobile genetic elements, are the most abundant genes in nature. While many bacteria encode an abundance of transposases in their genomes, the current paradigm is that the expression of transposase genes is tightly regulated and generally low due to its severe mutagenic effects. In the current study, we detected the highest number of transposase proteins ever reported in bacteria, in symbionts of the gutless marine worm Olavius algarvensis with metaproteomics. At least 26 different transposases from 12 different families were detected, and genomic and proteomic analyses suggest that many of these are active. This high expression of transposases indicates that the mechanisms for their tight regulation have been disabled or no longer exist. |
format | Online Article Text |
id | pubmed-3684830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | American Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-36848302013-06-19 Metaproteomics Reveals Abundant Transposase Expression in Mutualistic Endosymbionts Kleiner, Manuel Young, Jacque C. Shah, Manesh VerBerkmoes, Nathan C. Dubilier, Nicole mBio Observation Transposases, enzymes that catalyze the movement of mobile genetic elements, are the most abundant genes in nature. While many bacteria encode an abundance of transposases in their genomes, the current paradigm is that the expression of transposase genes is tightly regulated and generally low due to its severe mutagenic effects. In the current study, we detected the highest number of transposase proteins ever reported in bacteria, in symbionts of the gutless marine worm Olavius algarvensis with metaproteomics. At least 26 different transposases from 12 different families were detected, and genomic and proteomic analyses suggest that many of these are active. This high expression of transposases indicates that the mechanisms for their tight regulation have been disabled or no longer exist. American Society of Microbiology 2013-06-18 /pmc/articles/PMC3684830/ /pubmed/23781067 http://dx.doi.org/10.1128/mBio.00223-13 Text en Copyright © 2013 Kleiner et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Observation Kleiner, Manuel Young, Jacque C. Shah, Manesh VerBerkmoes, Nathan C. Dubilier, Nicole Metaproteomics Reveals Abundant Transposase Expression in Mutualistic Endosymbionts |
title | Metaproteomics Reveals Abundant Transposase Expression in Mutualistic Endosymbionts |
title_full | Metaproteomics Reveals Abundant Transposase Expression in Mutualistic Endosymbionts |
title_fullStr | Metaproteomics Reveals Abundant Transposase Expression in Mutualistic Endosymbionts |
title_full_unstemmed | Metaproteomics Reveals Abundant Transposase Expression in Mutualistic Endosymbionts |
title_short | Metaproteomics Reveals Abundant Transposase Expression in Mutualistic Endosymbionts |
title_sort | metaproteomics reveals abundant transposase expression in mutualistic endosymbionts |
topic | Observation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684830/ https://www.ncbi.nlm.nih.gov/pubmed/23781067 http://dx.doi.org/10.1128/mBio.00223-13 |
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