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Compartment-specific pH monitoring in Bacillus subtilis using fluorescent sensor proteins: a tool to analyze the antibacterial effect of weak organic acids

The internal pH (pH(i)) of a living cell is one of its most important physiological parameters. To monitor the pH inside Bacillus subtilis during various stages of its life cycle, we constructed an improved version (IpHluorin) of the ratiometric, pH-sensitive fluorescent protein pHluorin by extendin...

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Detalles Bibliográficos
Autores principales: van Beilen, Johan W. A., Brul, Stanley
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3685010/
https://www.ncbi.nlm.nih.gov/pubmed/23785365
http://dx.doi.org/10.3389/fmicb.2013.00157