Regeneration of multiple shoots from petiole callus of Viola serpens Wall

Experiments were conducted to develop methodology for in vitro propagation and rapid multiplication of Viola serpens Wall. using petiole explant. The MS medium supplemented with 2, 4-D (6.78 μm) was found most suitable for callus induction in petiole explant. The best growth response and higher rate...

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Autores principales: Vishwakarma, Umashankar Radheshyam, Gurav, Arun Manohar, Sharma, Prakash Chandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3685769/
https://www.ncbi.nlm.nih.gov/pubmed/23798882
http://dx.doi.org/10.4103/0974-8490.110533
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author Vishwakarma, Umashankar Radheshyam
Gurav, Arun Manohar
Sharma, Prakash Chandra
author_facet Vishwakarma, Umashankar Radheshyam
Gurav, Arun Manohar
Sharma, Prakash Chandra
author_sort Vishwakarma, Umashankar Radheshyam
collection PubMed
description Experiments were conducted to develop methodology for in vitro propagation and rapid multiplication of Viola serpens Wall. using petiole explant. The MS medium supplemented with 2, 4-D (6.78 μm) was found most suitable for callus induction in petiole explant. The best growth response and higher rate of shoot regeneration from petiole callus was observed on MS medium containing BAP (11.10 μm) as the average number of shoots could be increased to 36.4 on fourth successive subculturing. Higher rooting response with larger number of roots were observed in shoots inoculated on the half-strength MS medium supplemented with IBA (19.68 μm).
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spelling pubmed-36857692013-06-24 Regeneration of multiple shoots from petiole callus of Viola serpens Wall Vishwakarma, Umashankar Radheshyam Gurav, Arun Manohar Sharma, Prakash Chandra Pharmacognosy Res Original Article Experiments were conducted to develop methodology for in vitro propagation and rapid multiplication of Viola serpens Wall. using petiole explant. The MS medium supplemented with 2, 4-D (6.78 μm) was found most suitable for callus induction in petiole explant. The best growth response and higher rate of shoot regeneration from petiole callus was observed on MS medium containing BAP (11.10 μm) as the average number of shoots could be increased to 36.4 on fourth successive subculturing. Higher rooting response with larger number of roots were observed in shoots inoculated on the half-strength MS medium supplemented with IBA (19.68 μm). Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3685769/ /pubmed/23798882 http://dx.doi.org/10.4103/0974-8490.110533 Text en Copyright: © Pharmacognosy Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Vishwakarma, Umashankar Radheshyam
Gurav, Arun Manohar
Sharma, Prakash Chandra
Regeneration of multiple shoots from petiole callus of Viola serpens Wall
title Regeneration of multiple shoots from petiole callus of Viola serpens Wall
title_full Regeneration of multiple shoots from petiole callus of Viola serpens Wall
title_fullStr Regeneration of multiple shoots from petiole callus of Viola serpens Wall
title_full_unstemmed Regeneration of multiple shoots from petiole callus of Viola serpens Wall
title_short Regeneration of multiple shoots from petiole callus of Viola serpens Wall
title_sort regeneration of multiple shoots from petiole callus of viola serpens wall
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3685769/
https://www.ncbi.nlm.nih.gov/pubmed/23798882
http://dx.doi.org/10.4103/0974-8490.110533
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