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Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells

The 30K family of proteins is important in energy metabolism and may play a role in inhibiting cellular apoptosis in silkworms (Bombyx mori). Several 30K-family proteins have been identified. In this study, two new silkworm genes, referred to as Slp (NM 001126256) and Lsp-t (NM 001043443), were anal...

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Autores principales: Yu, Wei, Li, Qing, Yao, Yuhua, Quan, Yanping, Zhang, Yaozhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3686079/
https://www.ncbi.nlm.nih.gov/pubmed/23862133
http://dx.doi.org/10.1155/2013/323592
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author Yu, Wei
Li, Qing
Yao, Yuhua
Quan, Yanping
Zhang, Yaozhou
author_facet Yu, Wei
Li, Qing
Yao, Yuhua
Quan, Yanping
Zhang, Yaozhou
author_sort Yu, Wei
collection PubMed
description The 30K family of proteins is important in energy metabolism and may play a role in inhibiting cellular apoptosis in silkworms (Bombyx mori). Several 30K-family proteins have been identified. In this study, two new silkworm genes, referred to as Slp (NM 001126256) and Lsp-t (NM 001043443), were analyzed by a bioinformatics approach according to the sequences of 30K proteins previously reported in the silkworm. Both Slp and Lsp-t shared more than 41% amino acid sequence homology with the reported 30K proteins and displayed a conserved domain consistent with that of lipoprotein-11. Additionally, the cDNA sequences of both Slp and Lsp-t were obtained from the fat bodies of silkworm larvae by reverse transcription polymerase chain reaction. Both genes were expressed in BmN cells using the Bac-to-Bac system. Purified Slp and Lsp-t were added to cultured BmN and human umbilical vein endothelial cells (HUVEC) that were treated with H(2)O(2). Both Slp and Lsp-t significantly enhanced the viability and suppressed DNA fragmentation in H(2)O(2) treated BmN and HUVEC cells. This study suggested that Slp and Lsp-t exhibit similar biological activities as their known 30K-protein counterparts and mediate an inhibitory effect against H(2)O(2)-induced apoptosis.
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spelling pubmed-36860792013-07-16 Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells Yu, Wei Li, Qing Yao, Yuhua Quan, Yanping Zhang, Yaozhou Int J Genomics Research Article The 30K family of proteins is important in energy metabolism and may play a role in inhibiting cellular apoptosis in silkworms (Bombyx mori). Several 30K-family proteins have been identified. In this study, two new silkworm genes, referred to as Slp (NM 001126256) and Lsp-t (NM 001043443), were analyzed by a bioinformatics approach according to the sequences of 30K proteins previously reported in the silkworm. Both Slp and Lsp-t shared more than 41% amino acid sequence homology with the reported 30K proteins and displayed a conserved domain consistent with that of lipoprotein-11. Additionally, the cDNA sequences of both Slp and Lsp-t were obtained from the fat bodies of silkworm larvae by reverse transcription polymerase chain reaction. Both genes were expressed in BmN cells using the Bac-to-Bac system. Purified Slp and Lsp-t were added to cultured BmN and human umbilical vein endothelial cells (HUVEC) that were treated with H(2)O(2). Both Slp and Lsp-t significantly enhanced the viability and suppressed DNA fragmentation in H(2)O(2) treated BmN and HUVEC cells. This study suggested that Slp and Lsp-t exhibit similar biological activities as their known 30K-protein counterparts and mediate an inhibitory effect against H(2)O(2)-induced apoptosis. Hindawi Publishing Corporation 2013 2013-06-03 /pmc/articles/PMC3686079/ /pubmed/23862133 http://dx.doi.org/10.1155/2013/323592 Text en Copyright © 2013 Wei Yu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yu, Wei
Li, Qing
Yao, Yuhua
Quan, Yanping
Zhang, Yaozhou
Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells
title Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells
title_full Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells
title_fullStr Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells
title_full_unstemmed Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells
title_short Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells
title_sort two novel 30k proteins overexpressed in baculovirus system and their antiapoptotic effect in insect and mammalian cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3686079/
https://www.ncbi.nlm.nih.gov/pubmed/23862133
http://dx.doi.org/10.1155/2013/323592
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