Cargando…
The 2009 pandemic (H1N1) viruses isolated from pigs show enhanced pathogenicity in mice
Since the emergence of the 2009 pandemic (H1N1) virus (2009/H1N1) in April 2009, cases of transmission from humans to pigs have been reported frequently. In our previous studies, four 2009/H1N1 variants were isolated from pigs. To better understand the phenotypic differences of the pig isolates comp...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3686621/ https://www.ncbi.nlm.nih.gov/pubmed/23758678 http://dx.doi.org/10.1186/1297-9716-44-41 |
_version_ | 1782273803783503872 |
---|---|
author | Li, Yongtao Zou, Wei Jia, Guangmin Ke, Jianjiang Zhu, Jiping Lin, Xian Zhou, Hongbo Jin, Meilin |
author_facet | Li, Yongtao Zou, Wei Jia, Guangmin Ke, Jianjiang Zhu, Jiping Lin, Xian Zhou, Hongbo Jin, Meilin |
author_sort | Li, Yongtao |
collection | PubMed |
description | Since the emergence of the 2009 pandemic (H1N1) virus (2009/H1N1) in April 2009, cases of transmission from humans to pigs have been reported frequently. In our previous studies, four 2009/H1N1 variants were isolated from pigs. To better understand the phenotypic differences of the pig isolates compared with the human isolate, in this study mice were inoculated intranasally with different 2009/H1N1 viruses, and monitored for morbidity, mortality, and viral replication, cytokine production and pathological changes in the lungs. The results show that all isolates show effective replication in lungs, but varying in their ability to cause morbidity. In particular, the strains of A/swine/Nanchang/3/2010 (H1N1) and A/swine/Nanchang/F9/2010 (H1N1) show the greatest virulence with a persisting replication in lungs and high lethality for mice, compared with the human isolate A/Liaoning /14/2009 (H1N1), which shows low virulence in mice. Furthermore, the lethal strains could induce more severe lung pathological changes and higher production of cytokines than that of other strains at an early stage. Amino acid sequence analysis illustrates prominent differences in viral surface glycoproteins and polymerase subunits between pig isolates and human strains that might correlate with their phenotypic differences. These studies demonstrate that the 2009/H1N1 pig isolates exhibit heterogeneous infectivity and pathogencity in mice, and some strains possess an enhanced pathogenicity compared with the human isolate. |
format | Online Article Text |
id | pubmed-3686621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-36866212013-06-20 The 2009 pandemic (H1N1) viruses isolated from pigs show enhanced pathogenicity in mice Li, Yongtao Zou, Wei Jia, Guangmin Ke, Jianjiang Zhu, Jiping Lin, Xian Zhou, Hongbo Jin, Meilin Vet Res Research Since the emergence of the 2009 pandemic (H1N1) virus (2009/H1N1) in April 2009, cases of transmission from humans to pigs have been reported frequently. In our previous studies, four 2009/H1N1 variants were isolated from pigs. To better understand the phenotypic differences of the pig isolates compared with the human isolate, in this study mice were inoculated intranasally with different 2009/H1N1 viruses, and monitored for morbidity, mortality, and viral replication, cytokine production and pathological changes in the lungs. The results show that all isolates show effective replication in lungs, but varying in their ability to cause morbidity. In particular, the strains of A/swine/Nanchang/3/2010 (H1N1) and A/swine/Nanchang/F9/2010 (H1N1) show the greatest virulence with a persisting replication in lungs and high lethality for mice, compared with the human isolate A/Liaoning /14/2009 (H1N1), which shows low virulence in mice. Furthermore, the lethal strains could induce more severe lung pathological changes and higher production of cytokines than that of other strains at an early stage. Amino acid sequence analysis illustrates prominent differences in viral surface glycoproteins and polymerase subunits between pig isolates and human strains that might correlate with their phenotypic differences. These studies demonstrate that the 2009/H1N1 pig isolates exhibit heterogeneous infectivity and pathogencity in mice, and some strains possess an enhanced pathogenicity compared with the human isolate. BioMed Central 2013 2013-06-11 /pmc/articles/PMC3686621/ /pubmed/23758678 http://dx.doi.org/10.1186/1297-9716-44-41 Text en Copyright © 2013 Li et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Li, Yongtao Zou, Wei Jia, Guangmin Ke, Jianjiang Zhu, Jiping Lin, Xian Zhou, Hongbo Jin, Meilin The 2009 pandemic (H1N1) viruses isolated from pigs show enhanced pathogenicity in mice |
title | The 2009 pandemic (H1N1) viruses isolated from pigs show enhanced pathogenicity in mice |
title_full | The 2009 pandemic (H1N1) viruses isolated from pigs show enhanced pathogenicity in mice |
title_fullStr | The 2009 pandemic (H1N1) viruses isolated from pigs show enhanced pathogenicity in mice |
title_full_unstemmed | The 2009 pandemic (H1N1) viruses isolated from pigs show enhanced pathogenicity in mice |
title_short | The 2009 pandemic (H1N1) viruses isolated from pigs show enhanced pathogenicity in mice |
title_sort | 2009 pandemic (h1n1) viruses isolated from pigs show enhanced pathogenicity in mice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3686621/ https://www.ncbi.nlm.nih.gov/pubmed/23758678 http://dx.doi.org/10.1186/1297-9716-44-41 |
work_keys_str_mv | AT liyongtao the2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT zouwei the2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT jiaguangmin the2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT kejianjiang the2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT zhujiping the2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT linxian the2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT zhouhongbo the2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT jinmeilin the2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT liyongtao 2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT zouwei 2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT jiaguangmin 2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT kejianjiang 2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT zhujiping 2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT linxian 2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT zhouhongbo 2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice AT jinmeilin 2009pandemich1n1virusesisolatedfrompigsshowenhancedpathogenicityinmice |