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Protein tyrosine kinase 6 is associated with nasopharyngeal carcinoma poor prognosis and metastasis

BACKGROUND: The aim of this study was to analyze the expression of protein tyrosine kinase 6 (PTK6) in nasopharyngeal carcinoma (NPC) samples, and to identify whether PTK6 can serve as a biomarker for the diagnosis and prognosis of NPC. METHODS: We used quantitative RT-PCR and Western blotting analy...

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Autores principales: Liu, Li-na, Huang, Pei-yu, Lin, Zhi-rui, Hu, Li-juan, Liang, Jian-zhong, Li, Man-zhi, Tang, Lin-quan, Zeng, Mu-sheng, Zhong, Qian, Zeng, Bo-hang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3686693/
https://www.ncbi.nlm.nih.gov/pubmed/23758975
http://dx.doi.org/10.1186/1479-5876-11-140
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author Liu, Li-na
Huang, Pei-yu
Lin, Zhi-rui
Hu, Li-juan
Liang, Jian-zhong
Li, Man-zhi
Tang, Lin-quan
Zeng, Mu-sheng
Zhong, Qian
Zeng, Bo-hang
author_facet Liu, Li-na
Huang, Pei-yu
Lin, Zhi-rui
Hu, Li-juan
Liang, Jian-zhong
Li, Man-zhi
Tang, Lin-quan
Zeng, Mu-sheng
Zhong, Qian
Zeng, Bo-hang
author_sort Liu, Li-na
collection PubMed
description BACKGROUND: The aim of this study was to analyze the expression of protein tyrosine kinase 6 (PTK6) in nasopharyngeal carcinoma (NPC) samples, and to identify whether PTK6 can serve as a biomarker for the diagnosis and prognosis of NPC. METHODS: We used quantitative RT-PCR and Western blotting analysis to detect mRNA and protein expression of PTK6 in NPC cell lines and immortalized nasopharyngeal epithelial cell lines. 31 NPC and 16 non-tumorous nasopharyngeal mucosa biopsies were collected to detect the difference in the expression of mRNA level of PTK6 by quantitative RT-PCR. We also collected 178 NPC and 10 normal nasopharyngeal epithelial cases with clinical follow-up data to investigate the expression of PTK6 by immunohistochemistry staining (IHC). PTK6 overexpression on cell growth and colony formation ability were measured by the method of cell proliferation assay and colony formation assay. RESULTS: The expression of PTK6 was higher in most of NPC cell lines at both mRNA and protein levels than in immortalized nasopharyngeal epithelial cell lines (NPECs) induced by Bmi-1 (Bmi-1/NPEC1, and Bmi-1/NPEC2). The mRNA level of PTK6 was high in NPC biopsies compared to non-tumorous nasopharyngeal mucosa biopsies. IHC results showed the expression of PTK6 was significantly correlated to tumor size (P<0.001), clinical stage (P<0.001), and metastasis (P=0.016). The patients with high-expression of PTK6 had a significantly poor prognosis compared to those of low-expression (47.8% versus 80.0%, P<0.001), especially in the patients at the advanced stages (42.2% versus 79.1%, P<0.001). Multivariate analysis indicated that the level of PTK6 expression was an independent prognostic factor for the overall survival of patients with NPC (P <0.001). Overexpression of PTK6 in HNE1 cells enhanced the ability of cell proliferation and colony formation. CONCLUSIONS: Our results suggest that high-expression of PTK6 is an independent factor for NPC patients and it might serve as a potential prognostic biomarker for patients with NPC.
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spelling pubmed-36866932013-06-20 Protein tyrosine kinase 6 is associated with nasopharyngeal carcinoma poor prognosis and metastasis Liu, Li-na Huang, Pei-yu Lin, Zhi-rui Hu, Li-juan Liang, Jian-zhong Li, Man-zhi Tang, Lin-quan Zeng, Mu-sheng Zhong, Qian Zeng, Bo-hang J Transl Med Research BACKGROUND: The aim of this study was to analyze the expression of protein tyrosine kinase 6 (PTK6) in nasopharyngeal carcinoma (NPC) samples, and to identify whether PTK6 can serve as a biomarker for the diagnosis and prognosis of NPC. METHODS: We used quantitative RT-PCR and Western blotting analysis to detect mRNA and protein expression of PTK6 in NPC cell lines and immortalized nasopharyngeal epithelial cell lines. 31 NPC and 16 non-tumorous nasopharyngeal mucosa biopsies were collected to detect the difference in the expression of mRNA level of PTK6 by quantitative RT-PCR. We also collected 178 NPC and 10 normal nasopharyngeal epithelial cases with clinical follow-up data to investigate the expression of PTK6 by immunohistochemistry staining (IHC). PTK6 overexpression on cell growth and colony formation ability were measured by the method of cell proliferation assay and colony formation assay. RESULTS: The expression of PTK6 was higher in most of NPC cell lines at both mRNA and protein levels than in immortalized nasopharyngeal epithelial cell lines (NPECs) induced by Bmi-1 (Bmi-1/NPEC1, and Bmi-1/NPEC2). The mRNA level of PTK6 was high in NPC biopsies compared to non-tumorous nasopharyngeal mucosa biopsies. IHC results showed the expression of PTK6 was significantly correlated to tumor size (P<0.001), clinical stage (P<0.001), and metastasis (P=0.016). The patients with high-expression of PTK6 had a significantly poor prognosis compared to those of low-expression (47.8% versus 80.0%, P<0.001), especially in the patients at the advanced stages (42.2% versus 79.1%, P<0.001). Multivariate analysis indicated that the level of PTK6 expression was an independent prognostic factor for the overall survival of patients with NPC (P <0.001). Overexpression of PTK6 in HNE1 cells enhanced the ability of cell proliferation and colony formation. CONCLUSIONS: Our results suggest that high-expression of PTK6 is an independent factor for NPC patients and it might serve as a potential prognostic biomarker for patients with NPC. BioMed Central 2013-06-09 /pmc/articles/PMC3686693/ /pubmed/23758975 http://dx.doi.org/10.1186/1479-5876-11-140 Text en Copyright © 2013 Liu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Liu, Li-na
Huang, Pei-yu
Lin, Zhi-rui
Hu, Li-juan
Liang, Jian-zhong
Li, Man-zhi
Tang, Lin-quan
Zeng, Mu-sheng
Zhong, Qian
Zeng, Bo-hang
Protein tyrosine kinase 6 is associated with nasopharyngeal carcinoma poor prognosis and metastasis
title Protein tyrosine kinase 6 is associated with nasopharyngeal carcinoma poor prognosis and metastasis
title_full Protein tyrosine kinase 6 is associated with nasopharyngeal carcinoma poor prognosis and metastasis
title_fullStr Protein tyrosine kinase 6 is associated with nasopharyngeal carcinoma poor prognosis and metastasis
title_full_unstemmed Protein tyrosine kinase 6 is associated with nasopharyngeal carcinoma poor prognosis and metastasis
title_short Protein tyrosine kinase 6 is associated with nasopharyngeal carcinoma poor prognosis and metastasis
title_sort protein tyrosine kinase 6 is associated with nasopharyngeal carcinoma poor prognosis and metastasis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3686693/
https://www.ncbi.nlm.nih.gov/pubmed/23758975
http://dx.doi.org/10.1186/1479-5876-11-140
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