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Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer
AIM OF THE STUDY: Several epidemiological studies have attempted to demonstrate a relationship between increased serum level of insulin-like growth factor 1 (IGF-1) and an augmented risk of developing colorectal cancers (CRC). The human IGF-1 gene is composed of 6 exons and demonstrated expression o...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3687396/ https://www.ncbi.nlm.nih.gov/pubmed/23788868 http://dx.doi.org/10.5114/wo.2012.28794 |
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author | Kasprzak, Aldona Szaflarski, Witold Szmeja, Jacek Andrzejewska, Małgorzata Przybyszewska, Wiesława Koczorowska, Maria Drews, Michał Kaczmarek, Elżbieta |
author_facet | Kasprzak, Aldona Szaflarski, Witold Szmeja, Jacek Andrzejewska, Małgorzata Przybyszewska, Wiesława Koczorowska, Maria Drews, Michał Kaczmarek, Elżbieta |
author_sort | Kasprzak, Aldona |
collection | PubMed |
description | AIM OF THE STUDY: Several epidemiological studies have attempted to demonstrate a relationship between increased serum level of insulin-like growth factor 1 (IGF-1) and an augmented risk of developing colorectal cancers (CRC). The human IGF-1 gene is composed of 6 exons and demonstrated expression of 6 different splice variants (isoforms) of mRNA (IA, IB, IC, IIA, IIB and IIC). The aim of the study was to evaluate the expression of different isoforms of IGF-1 mRNA in CRC and normal colon tissue. MATERIAL AND METHODS: 13 paired tissue specimens (colorectal tumor and non-tumor tissues) were analyzed using both quantitative polymerase chain reaction (PCR) and immunocytochemistry methods (IHC). The expression of classes I and II and variants A, B, C of IGF-1 mRNA were measured. RESULTS: In CRC higher amounts of IGF-1 class II mRNA than class I mRNA were detected. Among A, B, C isoforms, A variant of IGF-1 mRNA prevailed. The amounts of IGF-1 class I and class II mRNAs and of IGF-1 variant B mRNA were lowered in CRC as compared to the control. In CRC significant correlations were detected between reciprocal expression of class I and class II as well as between I and II isoforms and A, B and C. CONCLUSIONS: Expression of IGF-1 mRNA isoforms differs between normal and CRC tissues. Even if all isoforms of IGF-1 mRNA manifested correlations with each other in tissues of CRC, expression of all transcripts (except that of isoform A) was significantly decreased as compared to the control. |
format | Online Article Text |
id | pubmed-3687396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-36873962013-06-20 Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer Kasprzak, Aldona Szaflarski, Witold Szmeja, Jacek Andrzejewska, Małgorzata Przybyszewska, Wiesława Koczorowska, Maria Drews, Michał Kaczmarek, Elżbieta Contemp Oncol (Pozn) Original Paper AIM OF THE STUDY: Several epidemiological studies have attempted to demonstrate a relationship between increased serum level of insulin-like growth factor 1 (IGF-1) and an augmented risk of developing colorectal cancers (CRC). The human IGF-1 gene is composed of 6 exons and demonstrated expression of 6 different splice variants (isoforms) of mRNA (IA, IB, IC, IIA, IIB and IIC). The aim of the study was to evaluate the expression of different isoforms of IGF-1 mRNA in CRC and normal colon tissue. MATERIAL AND METHODS: 13 paired tissue specimens (colorectal tumor and non-tumor tissues) were analyzed using both quantitative polymerase chain reaction (PCR) and immunocytochemistry methods (IHC). The expression of classes I and II and variants A, B, C of IGF-1 mRNA were measured. RESULTS: In CRC higher amounts of IGF-1 class II mRNA than class I mRNA were detected. Among A, B, C isoforms, A variant of IGF-1 mRNA prevailed. The amounts of IGF-1 class I and class II mRNAs and of IGF-1 variant B mRNA were lowered in CRC as compared to the control. In CRC significant correlations were detected between reciprocal expression of class I and class II as well as between I and II isoforms and A, B and C. CONCLUSIONS: Expression of IGF-1 mRNA isoforms differs between normal and CRC tissues. Even if all isoforms of IGF-1 mRNA manifested correlations with each other in tissues of CRC, expression of all transcripts (except that of isoform A) was significantly decreased as compared to the control. Termedia Publishing House 2012-05-29 2012 /pmc/articles/PMC3687396/ /pubmed/23788868 http://dx.doi.org/10.5114/wo.2012.28794 Text en Copyright © 2012 Termedia http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Paper Kasprzak, Aldona Szaflarski, Witold Szmeja, Jacek Andrzejewska, Małgorzata Przybyszewska, Wiesława Koczorowska, Maria Drews, Michał Kaczmarek, Elżbieta Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer |
title | Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer |
title_full | Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer |
title_fullStr | Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer |
title_full_unstemmed | Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer |
title_short | Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer |
title_sort | expression of various insulin-like growth factor-1 mrna isoforms in colorectal cancer |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3687396/ https://www.ncbi.nlm.nih.gov/pubmed/23788868 http://dx.doi.org/10.5114/wo.2012.28794 |
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