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Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering

We aimed to develop an alginate hydrogel (AH) modified with nano-/microfibers of titanium dioxide (nfTD) and hydroxyapatite (nfHY) and evaluated its biological and chemical properties. Nano-/microfibers of nfTD and nfHY were combined with AH, and its chemical properties were evaluated by FTIR spectr...

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Autores principales: Palma Santana, Bianca, Nedel, Fernanda, Piva, Evandro, Varella de Carvalho, Rodrigo, Fernando Demarco, Flávio, Lenin Villarreal Carreño, Neftali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3687604/
https://www.ncbi.nlm.nih.gov/pubmed/23862142
http://dx.doi.org/10.1155/2013/307602
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author Palma Santana, Bianca
Nedel, Fernanda
Piva, Evandro
Varella de Carvalho, Rodrigo
Fernando Demarco, Flávio
Lenin Villarreal Carreño, Neftali
author_facet Palma Santana, Bianca
Nedel, Fernanda
Piva, Evandro
Varella de Carvalho, Rodrigo
Fernando Demarco, Flávio
Lenin Villarreal Carreño, Neftali
author_sort Palma Santana, Bianca
collection PubMed
description We aimed to develop an alginate hydrogel (AH) modified with nano-/microfibers of titanium dioxide (nfTD) and hydroxyapatite (nfHY) and evaluated its biological and chemical properties. Nano-/microfibers of nfTD and nfHY were combined with AH, and its chemical properties were evaluated by FTIR spectroscopy, X-ray diffraction, energy dispersive X-Ray analysis, and the cytocompatibility by the WST-1 assay. The results demonstrate that the association of nfTD and nfHY nano-/microfibers to AH did not modified the chemical characteristics of the scaffold and that the association was not cytotoxic. In the first 3 h of culture with NIH/3T3 cells nfHY AH scaffolds showed a slight increase in cell viability when compared to AH alone or associated with nfTD. However, an increase in cell viability was observed in 24 h when nfTD was associated with AH scaffold. In conclusion our study demonstrates that the combination of nfHY and nfTD nano-/microfibers in AH scaffold maintains the chemical characteristics of alginate and that this association is cytocompatible. Additionally the combination of nfHY with AH favored cell viability in a short term, and the addition of nfTD increased cell viability in a long term.
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spelling pubmed-36876042013-07-16 Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering Palma Santana, Bianca Nedel, Fernanda Piva, Evandro Varella de Carvalho, Rodrigo Fernando Demarco, Flávio Lenin Villarreal Carreño, Neftali Biomed Res Int Research Article We aimed to develop an alginate hydrogel (AH) modified with nano-/microfibers of titanium dioxide (nfTD) and hydroxyapatite (nfHY) and evaluated its biological and chemical properties. Nano-/microfibers of nfTD and nfHY were combined with AH, and its chemical properties were evaluated by FTIR spectroscopy, X-ray diffraction, energy dispersive X-Ray analysis, and the cytocompatibility by the WST-1 assay. The results demonstrate that the association of nfTD and nfHY nano-/microfibers to AH did not modified the chemical characteristics of the scaffold and that the association was not cytotoxic. In the first 3 h of culture with NIH/3T3 cells nfHY AH scaffolds showed a slight increase in cell viability when compared to AH alone or associated with nfTD. However, an increase in cell viability was observed in 24 h when nfTD was associated with AH scaffold. In conclusion our study demonstrates that the combination of nfHY and nfTD nano-/microfibers in AH scaffold maintains the chemical characteristics of alginate and that this association is cytocompatible. Additionally the combination of nfHY with AH favored cell viability in a short term, and the addition of nfTD increased cell viability in a long term. Hindawi Publishing Corporation 2013 2013-06-05 /pmc/articles/PMC3687604/ /pubmed/23862142 http://dx.doi.org/10.1155/2013/307602 Text en Copyright © 2013 Bianca Palma Santana et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Palma Santana, Bianca
Nedel, Fernanda
Piva, Evandro
Varella de Carvalho, Rodrigo
Fernando Demarco, Flávio
Lenin Villarreal Carreño, Neftali
Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering
title Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering
title_full Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering
title_fullStr Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering
title_full_unstemmed Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering
title_short Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering
title_sort preparation, modification, and characterization of alginate hydrogel with nano-/microfibers: a new perspective for tissue engineering
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3687604/
https://www.ncbi.nlm.nih.gov/pubmed/23862142
http://dx.doi.org/10.1155/2013/307602
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