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Cardiovascular Changes in Atherosclerotic ApoE-Deficient Mice Exposed to Co60 (γ) Radiation

BACKGROUND: There is evidence for a role of ionizing radiation in cardiovascular diseases. The goal of this work was to identify changes in oxidative and nitrative stress pathways and the status of the endothelinergic system during progression of atherosclerosis in ApoE-deficient mice after single a...

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Autores principales: Kumarathasan, Prem, Vincent, Renaud, Blais, Erica, Saravanamuthu, Anu, Gupta, Pallavi, Wyatt, Heather, Mitchel, Ronald, Hannan, Mohammed, Trivedi, Akilesh, Whitman, Stewart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688723/
https://www.ncbi.nlm.nih.gov/pubmed/23840332
http://dx.doi.org/10.1371/journal.pone.0065486
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author Kumarathasan, Prem
Vincent, Renaud
Blais, Erica
Saravanamuthu, Anu
Gupta, Pallavi
Wyatt, Heather
Mitchel, Ronald
Hannan, Mohammed
Trivedi, Akilesh
Whitman, Stewart
author_facet Kumarathasan, Prem
Vincent, Renaud
Blais, Erica
Saravanamuthu, Anu
Gupta, Pallavi
Wyatt, Heather
Mitchel, Ronald
Hannan, Mohammed
Trivedi, Akilesh
Whitman, Stewart
author_sort Kumarathasan, Prem
collection PubMed
description BACKGROUND: There is evidence for a role of ionizing radiation in cardiovascular diseases. The goal of this work was to identify changes in oxidative and nitrative stress pathways and the status of the endothelinergic system during progression of atherosclerosis in ApoE-deficient mice after single and repeated exposure to ionizing radiation. METHODS AND RESULTS: B6.129P2-ApoE tmlUnc mice on a low-fat diet were acutely exposed (whole body) to Co60 (γ) (single dose 0, 0.5, and 2 Gy) at a dose rate of 36.32 cGy/min, or repeatedly (cumulative dose 0 and 2 Gy) at a dose-rate of 0.1 cGy/min for 5 d/wk, over a period of 4 weeks. Biological endpoints were investigated after 3–6 months of recovery post-radiation. The nitrative stress marker 3-nitrotyrosine and the vasoregulator peptides endothelin-1 and endothelin-3 in plasma were increased (p<0.05) in a dose-dependent manner 3–6 months after acute or chronic exposure to radiation. The oxidative stress marker 8-isoprostane was not affected by radiation, while plasma 8-hydroxydeoxyguanosine and L-3,4-dihydroxyphenylalanine decreased (p<0.05) after treatment. At 2Gy radiation dose, serum cholesterol was increased (p = 0.008) relative to controls. Percent lesion area increased (p = 0.005) with age of animal, but not with radiation treatment. CONCLUSIONS: Our observations are consistent with persistent nitrative stress and activation of the endothelinergic system in ApoE−/− mice after low-level ionizing radiation exposures. These mechanisms are known factors in the progression of atherosclerosis and other cardiovascular diseases.
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spelling pubmed-36887232013-07-09 Cardiovascular Changes in Atherosclerotic ApoE-Deficient Mice Exposed to Co60 (γ) Radiation Kumarathasan, Prem Vincent, Renaud Blais, Erica Saravanamuthu, Anu Gupta, Pallavi Wyatt, Heather Mitchel, Ronald Hannan, Mohammed Trivedi, Akilesh Whitman, Stewart PLoS One Research Article BACKGROUND: There is evidence for a role of ionizing radiation in cardiovascular diseases. The goal of this work was to identify changes in oxidative and nitrative stress pathways and the status of the endothelinergic system during progression of atherosclerosis in ApoE-deficient mice after single and repeated exposure to ionizing radiation. METHODS AND RESULTS: B6.129P2-ApoE tmlUnc mice on a low-fat diet were acutely exposed (whole body) to Co60 (γ) (single dose 0, 0.5, and 2 Gy) at a dose rate of 36.32 cGy/min, or repeatedly (cumulative dose 0 and 2 Gy) at a dose-rate of 0.1 cGy/min for 5 d/wk, over a period of 4 weeks. Biological endpoints were investigated after 3–6 months of recovery post-radiation. The nitrative stress marker 3-nitrotyrosine and the vasoregulator peptides endothelin-1 and endothelin-3 in plasma were increased (p<0.05) in a dose-dependent manner 3–6 months after acute or chronic exposure to radiation. The oxidative stress marker 8-isoprostane was not affected by radiation, while plasma 8-hydroxydeoxyguanosine and L-3,4-dihydroxyphenylalanine decreased (p<0.05) after treatment. At 2Gy radiation dose, serum cholesterol was increased (p = 0.008) relative to controls. Percent lesion area increased (p = 0.005) with age of animal, but not with radiation treatment. CONCLUSIONS: Our observations are consistent with persistent nitrative stress and activation of the endothelinergic system in ApoE−/− mice after low-level ionizing radiation exposures. These mechanisms are known factors in the progression of atherosclerosis and other cardiovascular diseases. Public Library of Science 2013-06-20 /pmc/articles/PMC3688723/ /pubmed/23840332 http://dx.doi.org/10.1371/journal.pone.0065486 Text en © 2013 Kumarathasan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kumarathasan, Prem
Vincent, Renaud
Blais, Erica
Saravanamuthu, Anu
Gupta, Pallavi
Wyatt, Heather
Mitchel, Ronald
Hannan, Mohammed
Trivedi, Akilesh
Whitman, Stewart
Cardiovascular Changes in Atherosclerotic ApoE-Deficient Mice Exposed to Co60 (γ) Radiation
title Cardiovascular Changes in Atherosclerotic ApoE-Deficient Mice Exposed to Co60 (γ) Radiation
title_full Cardiovascular Changes in Atherosclerotic ApoE-Deficient Mice Exposed to Co60 (γ) Radiation
title_fullStr Cardiovascular Changes in Atherosclerotic ApoE-Deficient Mice Exposed to Co60 (γ) Radiation
title_full_unstemmed Cardiovascular Changes in Atherosclerotic ApoE-Deficient Mice Exposed to Co60 (γ) Radiation
title_short Cardiovascular Changes in Atherosclerotic ApoE-Deficient Mice Exposed to Co60 (γ) Radiation
title_sort cardiovascular changes in atherosclerotic apoe-deficient mice exposed to co60 (γ) radiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688723/
https://www.ncbi.nlm.nih.gov/pubmed/23840332
http://dx.doi.org/10.1371/journal.pone.0065486
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