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A Large Plasmodium vivax Reservoir and Little Population Structure in the South Pacific

INTRODUCTION: The importance of Plasmodium vivax in malaria elimination is increasingly being recognized, yet little is known about its population size and population genetic structure in the South Pacific, an area that is the focus of intensified malaria control. METHODS: We have genotyped 13 micro...

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Autores principales: Koepfli, Cristian, Timinao, Lincoln, Antao, Tiago, Barry, Alyssa E., Siba, Peter, Mueller, Ivo, Felger, Ingrid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688846/
https://www.ncbi.nlm.nih.gov/pubmed/23823758
http://dx.doi.org/10.1371/journal.pone.0066041
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author Koepfli, Cristian
Timinao, Lincoln
Antao, Tiago
Barry, Alyssa E.
Siba, Peter
Mueller, Ivo
Felger, Ingrid
author_facet Koepfli, Cristian
Timinao, Lincoln
Antao, Tiago
Barry, Alyssa E.
Siba, Peter
Mueller, Ivo
Felger, Ingrid
author_sort Koepfli, Cristian
collection PubMed
description INTRODUCTION: The importance of Plasmodium vivax in malaria elimination is increasingly being recognized, yet little is known about its population size and population genetic structure in the South Pacific, an area that is the focus of intensified malaria control. METHODS: We have genotyped 13 microsatellite markers in 295 P. vivax isolates from four geographically distinct sites in Papua New Guinea (PNG) and one site from Solomon Islands, representing different transmission intensities. RESULTS: Diversity was very high with expected heterozygosity values ranging from 0.62 to 0.98 for the different markers. Effective population size was high (12′872 to 19′533 per site). In PNG population structuring was limited with moderate levels of genetic differentiation. F (ST) values (adjusted for high diversity of markers) were 0.14–0.15. Slightly higher levels were observed between PNG populations and Solomon Islands (F (ST) = 0.16). CONCLUSIONS: Low levels of population structure despite geographical barriers to transmission are in sharp contrast to results from regions of low P. vivax endemicity. Prior to intensification of malaria control programs in the study area, parasite diversity and effective population size remained high.
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spelling pubmed-36888462013-07-02 A Large Plasmodium vivax Reservoir and Little Population Structure in the South Pacific Koepfli, Cristian Timinao, Lincoln Antao, Tiago Barry, Alyssa E. Siba, Peter Mueller, Ivo Felger, Ingrid PLoS One Research Article INTRODUCTION: The importance of Plasmodium vivax in malaria elimination is increasingly being recognized, yet little is known about its population size and population genetic structure in the South Pacific, an area that is the focus of intensified malaria control. METHODS: We have genotyped 13 microsatellite markers in 295 P. vivax isolates from four geographically distinct sites in Papua New Guinea (PNG) and one site from Solomon Islands, representing different transmission intensities. RESULTS: Diversity was very high with expected heterozygosity values ranging from 0.62 to 0.98 for the different markers. Effective population size was high (12′872 to 19′533 per site). In PNG population structuring was limited with moderate levels of genetic differentiation. F (ST) values (adjusted for high diversity of markers) were 0.14–0.15. Slightly higher levels were observed between PNG populations and Solomon Islands (F (ST) = 0.16). CONCLUSIONS: Low levels of population structure despite geographical barriers to transmission are in sharp contrast to results from regions of low P. vivax endemicity. Prior to intensification of malaria control programs in the study area, parasite diversity and effective population size remained high. Public Library of Science 2013-06-18 /pmc/articles/PMC3688846/ /pubmed/23823758 http://dx.doi.org/10.1371/journal.pone.0066041 Text en © 2013 Koepfli et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Koepfli, Cristian
Timinao, Lincoln
Antao, Tiago
Barry, Alyssa E.
Siba, Peter
Mueller, Ivo
Felger, Ingrid
A Large Plasmodium vivax Reservoir and Little Population Structure in the South Pacific
title A Large Plasmodium vivax Reservoir and Little Population Structure in the South Pacific
title_full A Large Plasmodium vivax Reservoir and Little Population Structure in the South Pacific
title_fullStr A Large Plasmodium vivax Reservoir and Little Population Structure in the South Pacific
title_full_unstemmed A Large Plasmodium vivax Reservoir and Little Population Structure in the South Pacific
title_short A Large Plasmodium vivax Reservoir and Little Population Structure in the South Pacific
title_sort large plasmodium vivax reservoir and little population structure in the south pacific
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688846/
https://www.ncbi.nlm.nih.gov/pubmed/23823758
http://dx.doi.org/10.1371/journal.pone.0066041
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