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Galectin-4 Reduces Migration and Metastasis Formation of Pancreatic Cancer Cells
Galectin-4 (Gal-4) is a member of the galectin family of glycan binding proteins that shows a significantly higher expression in cystic tumors of the human pancreas and in pancreatic adenocarcinomas compared to normal pancreas. However, the putative function of Gal-4 in tumor progression of pancreat...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688853/ https://www.ncbi.nlm.nih.gov/pubmed/23824659 http://dx.doi.org/10.1371/journal.pone.0065957 |
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author | Belo, Ana I. van der Sar, Astrid M. Tefsen, Boris van Die, Irma |
author_facet | Belo, Ana I. van der Sar, Astrid M. Tefsen, Boris van Die, Irma |
author_sort | Belo, Ana I. |
collection | PubMed |
description | Galectin-4 (Gal-4) is a member of the galectin family of glycan binding proteins that shows a significantly higher expression in cystic tumors of the human pancreas and in pancreatic adenocarcinomas compared to normal pancreas. However, the putative function of Gal-4 in tumor progression of pancreatic cancer is still incompletely understood. In this study the role of Gal-4 in cancer progression was investigated, using a set of defined pancreatic cancer cell lines, Pa-Tu-8988S (PaTu-S) and Pa-Tu-8988T (PaTu-T), as a model. These two cell lines are derived from the same liver metastasis of a human primary pancreatic adenocarcinoma, but differ in their growth characteristics and metastatic capacity. We demonstrated that Gal-4 expression is high in PaTu-S, which shows poor migratory properties, whereas much lower Gal-4 levels are observed in the highly metastatic cell line PaTu-T. In PaTu-S, Gal-4 is found in the cytoplasm, but it is also secreted and accumulates at the membrane at sites of contact with neighboring cells. Moreover, we show that Gal-4 inhibits metastasis formation by delaying migration of pancreatic cancer cells in vitro using a scratch assay, and in vivo using zebrafish (Danio rerio) as an experimental model. Our data suggest that Gal-4 may act at the cell-surface of PaTu-S as an adhesion molecule to prevent release of the tumor cells, but has in addition a cytosolic function by inhibiting migration via a yet unknown mechanism. |
format | Online Article Text |
id | pubmed-3688853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36888532013-07-02 Galectin-4 Reduces Migration and Metastasis Formation of Pancreatic Cancer Cells Belo, Ana I. van der Sar, Astrid M. Tefsen, Boris van Die, Irma PLoS One Research Article Galectin-4 (Gal-4) is a member of the galectin family of glycan binding proteins that shows a significantly higher expression in cystic tumors of the human pancreas and in pancreatic adenocarcinomas compared to normal pancreas. However, the putative function of Gal-4 in tumor progression of pancreatic cancer is still incompletely understood. In this study the role of Gal-4 in cancer progression was investigated, using a set of defined pancreatic cancer cell lines, Pa-Tu-8988S (PaTu-S) and Pa-Tu-8988T (PaTu-T), as a model. These two cell lines are derived from the same liver metastasis of a human primary pancreatic adenocarcinoma, but differ in their growth characteristics and metastatic capacity. We demonstrated that Gal-4 expression is high in PaTu-S, which shows poor migratory properties, whereas much lower Gal-4 levels are observed in the highly metastatic cell line PaTu-T. In PaTu-S, Gal-4 is found in the cytoplasm, but it is also secreted and accumulates at the membrane at sites of contact with neighboring cells. Moreover, we show that Gal-4 inhibits metastasis formation by delaying migration of pancreatic cancer cells in vitro using a scratch assay, and in vivo using zebrafish (Danio rerio) as an experimental model. Our data suggest that Gal-4 may act at the cell-surface of PaTu-S as an adhesion molecule to prevent release of the tumor cells, but has in addition a cytosolic function by inhibiting migration via a yet unknown mechanism. Public Library of Science 2013-06-18 /pmc/articles/PMC3688853/ /pubmed/23824659 http://dx.doi.org/10.1371/journal.pone.0065957 Text en © 2013 Belo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Belo, Ana I. van der Sar, Astrid M. Tefsen, Boris van Die, Irma Galectin-4 Reduces Migration and Metastasis Formation of Pancreatic Cancer Cells |
title | Galectin-4 Reduces Migration and Metastasis Formation of Pancreatic Cancer Cells |
title_full | Galectin-4 Reduces Migration and Metastasis Formation of Pancreatic Cancer Cells |
title_fullStr | Galectin-4 Reduces Migration and Metastasis Formation of Pancreatic Cancer Cells |
title_full_unstemmed | Galectin-4 Reduces Migration and Metastasis Formation of Pancreatic Cancer Cells |
title_short | Galectin-4 Reduces Migration and Metastasis Formation of Pancreatic Cancer Cells |
title_sort | galectin-4 reduces migration and metastasis formation of pancreatic cancer cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688853/ https://www.ncbi.nlm.nih.gov/pubmed/23824659 http://dx.doi.org/10.1371/journal.pone.0065957 |
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