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Phytoplasma-Responsive microRNAs Modulate Hormonal, Nutritional, and Stress Signalling Pathways in Mexican Lime Trees

BACKGROUND: Witches’ broom disease of Mexican lime (Citrus aurantifolia L.), which is associated to the phytoplasma ‘Candidatus Phytoplasma aurantifolia’, is a devastating disease that results in significant economic losses. Plants adapt to biotic stresses by regulating gene expression at the transc...

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Autores principales: Ehya, Farveh, Monavarfeshani, Aboozar, Mohseni Fard, Ehsan, Karimi Farsad, Laleh, Khayam Nekouei, Mojtaba, Mardi, Mohsen, Salekdeh, Ghasem Hosseini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688891/
https://www.ncbi.nlm.nih.gov/pubmed/23824690
http://dx.doi.org/10.1371/journal.pone.0066372
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author Ehya, Farveh
Monavarfeshani, Aboozar
Mohseni Fard, Ehsan
Karimi Farsad, Laleh
Khayam Nekouei, Mojtaba
Mardi, Mohsen
Salekdeh, Ghasem Hosseini
author_facet Ehya, Farveh
Monavarfeshani, Aboozar
Mohseni Fard, Ehsan
Karimi Farsad, Laleh
Khayam Nekouei, Mojtaba
Mardi, Mohsen
Salekdeh, Ghasem Hosseini
author_sort Ehya, Farveh
collection PubMed
description BACKGROUND: Witches’ broom disease of Mexican lime (Citrus aurantifolia L.), which is associated to the phytoplasma ‘Candidatus Phytoplasma aurantifolia’, is a devastating disease that results in significant economic losses. Plants adapt to biotic stresses by regulating gene expression at the transcriptional and post-transcriptional levels. MicroRNAs (miRNAs) are a recently identified family of molecules that regulate plant responses to environmental stresses through post-transcriptional gene silencing. METHODS: Using a high-throughput approach to sequence small RNAs, we compared the expression profiles of miRNAs in healthy Mexican lime trees and in plants infected with ‘Ca. P. aurantifolia’. RESULTS: Our results demonstrated the involvement of different miRNAs in the response of Mexican lime trees to infection by ‘Ca. P. aurantifolia’. We identified miRNA families that are expressed differentially upon infection with phytoplasmas. Most of the miRNAs had variants with small sequence variations (isomiRs), which are expressed differentially in response to pathogen infection. CONCLUSIONS: It is likely that the miRNAs that are expressed differentially in healthy and phytoplasma-infected Mexican lime trees are involved in coordinating the regulation of hormonal, nutritional, and stress signalling pathways, and the complex interactions between them. Future research to elucidate the roles of these miRNAs should improve our understanding of the level of diversity of specific plant responses to phytoplasmas.
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spelling pubmed-36888912013-07-02 Phytoplasma-Responsive microRNAs Modulate Hormonal, Nutritional, and Stress Signalling Pathways in Mexican Lime Trees Ehya, Farveh Monavarfeshani, Aboozar Mohseni Fard, Ehsan Karimi Farsad, Laleh Khayam Nekouei, Mojtaba Mardi, Mohsen Salekdeh, Ghasem Hosseini PLoS One Research Article BACKGROUND: Witches’ broom disease of Mexican lime (Citrus aurantifolia L.), which is associated to the phytoplasma ‘Candidatus Phytoplasma aurantifolia’, is a devastating disease that results in significant economic losses. Plants adapt to biotic stresses by regulating gene expression at the transcriptional and post-transcriptional levels. MicroRNAs (miRNAs) are a recently identified family of molecules that regulate plant responses to environmental stresses through post-transcriptional gene silencing. METHODS: Using a high-throughput approach to sequence small RNAs, we compared the expression profiles of miRNAs in healthy Mexican lime trees and in plants infected with ‘Ca. P. aurantifolia’. RESULTS: Our results demonstrated the involvement of different miRNAs in the response of Mexican lime trees to infection by ‘Ca. P. aurantifolia’. We identified miRNA families that are expressed differentially upon infection with phytoplasmas. Most of the miRNAs had variants with small sequence variations (isomiRs), which are expressed differentially in response to pathogen infection. CONCLUSIONS: It is likely that the miRNAs that are expressed differentially in healthy and phytoplasma-infected Mexican lime trees are involved in coordinating the regulation of hormonal, nutritional, and stress signalling pathways, and the complex interactions between them. Future research to elucidate the roles of these miRNAs should improve our understanding of the level of diversity of specific plant responses to phytoplasmas. Public Library of Science 2013-06-18 /pmc/articles/PMC3688891/ /pubmed/23824690 http://dx.doi.org/10.1371/journal.pone.0066372 Text en © 2013 Ehya et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ehya, Farveh
Monavarfeshani, Aboozar
Mohseni Fard, Ehsan
Karimi Farsad, Laleh
Khayam Nekouei, Mojtaba
Mardi, Mohsen
Salekdeh, Ghasem Hosseini
Phytoplasma-Responsive microRNAs Modulate Hormonal, Nutritional, and Stress Signalling Pathways in Mexican Lime Trees
title Phytoplasma-Responsive microRNAs Modulate Hormonal, Nutritional, and Stress Signalling Pathways in Mexican Lime Trees
title_full Phytoplasma-Responsive microRNAs Modulate Hormonal, Nutritional, and Stress Signalling Pathways in Mexican Lime Trees
title_fullStr Phytoplasma-Responsive microRNAs Modulate Hormonal, Nutritional, and Stress Signalling Pathways in Mexican Lime Trees
title_full_unstemmed Phytoplasma-Responsive microRNAs Modulate Hormonal, Nutritional, and Stress Signalling Pathways in Mexican Lime Trees
title_short Phytoplasma-Responsive microRNAs Modulate Hormonal, Nutritional, and Stress Signalling Pathways in Mexican Lime Trees
title_sort phytoplasma-responsive micrornas modulate hormonal, nutritional, and stress signalling pathways in mexican lime trees
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688891/
https://www.ncbi.nlm.nih.gov/pubmed/23824690
http://dx.doi.org/10.1371/journal.pone.0066372
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