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Sox2 Level Is a Determinant of Cellular Reprogramming Potential
Epiblast stem cells (EpiSCs) and embryonic stem cells (ESCs) differ in their in vivo differentiation potential. While ESCs form teratomas and efficiently contribute to the development of chimeras, EpiSCs form teratomas but very rarely chimeras. In contrast to their differentiation potential, the rep...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688988/ https://www.ncbi.nlm.nih.gov/pubmed/23825671 http://dx.doi.org/10.1371/journal.pone.0067594 |
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author | Han, Dong Wook Tapia, Natalia Araúzo-Bravo, Marcos J. Lim, Kyung Tae Kim, Kee Pyo Ko, Kinarm Lee, Hoon Taek Schöler, Hans R. |
author_facet | Han, Dong Wook Tapia, Natalia Araúzo-Bravo, Marcos J. Lim, Kyung Tae Kim, Kee Pyo Ko, Kinarm Lee, Hoon Taek Schöler, Hans R. |
author_sort | Han, Dong Wook |
collection | PubMed |
description | Epiblast stem cells (EpiSCs) and embryonic stem cells (ESCs) differ in their in vivo differentiation potential. While ESCs form teratomas and efficiently contribute to the development of chimeras, EpiSCs form teratomas but very rarely chimeras. In contrast to their differentiation potential, the reprogramming potential of EpiSCs has not yet been investigated. Here we demonstrate that the epiblast-derived pluripotent stem cells EpiSCs and P19 embryonal carcinoma cells (ECCs) exhibit a lower reprogramming potential than ESCs and F9 ECCs. In addition, we show that the low reprogramming ability is due to the lower levels of Sox2 in epiblast-derived stem cells. Consistent with this observation, overexpression of Sox2 enhances reprogramming efficiency. In summary, these findings suggest that a low reprogramming potential is a general feature of epiblast-derived stem cells and that the Sox2 level is a determinant of the cellular reprogramming potential. |
format | Online Article Text |
id | pubmed-3688988 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36889882013-07-02 Sox2 Level Is a Determinant of Cellular Reprogramming Potential Han, Dong Wook Tapia, Natalia Araúzo-Bravo, Marcos J. Lim, Kyung Tae Kim, Kee Pyo Ko, Kinarm Lee, Hoon Taek Schöler, Hans R. PLoS One Research Article Epiblast stem cells (EpiSCs) and embryonic stem cells (ESCs) differ in their in vivo differentiation potential. While ESCs form teratomas and efficiently contribute to the development of chimeras, EpiSCs form teratomas but very rarely chimeras. In contrast to their differentiation potential, the reprogramming potential of EpiSCs has not yet been investigated. Here we demonstrate that the epiblast-derived pluripotent stem cells EpiSCs and P19 embryonal carcinoma cells (ECCs) exhibit a lower reprogramming potential than ESCs and F9 ECCs. In addition, we show that the low reprogramming ability is due to the lower levels of Sox2 in epiblast-derived stem cells. Consistent with this observation, overexpression of Sox2 enhances reprogramming efficiency. In summary, these findings suggest that a low reprogramming potential is a general feature of epiblast-derived stem cells and that the Sox2 level is a determinant of the cellular reprogramming potential. Public Library of Science 2013-06-18 /pmc/articles/PMC3688988/ /pubmed/23825671 http://dx.doi.org/10.1371/journal.pone.0067594 Text en © 2013 Han et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Han, Dong Wook Tapia, Natalia Araúzo-Bravo, Marcos J. Lim, Kyung Tae Kim, Kee Pyo Ko, Kinarm Lee, Hoon Taek Schöler, Hans R. Sox2 Level Is a Determinant of Cellular Reprogramming Potential |
title | Sox2 Level Is a Determinant of Cellular Reprogramming Potential |
title_full | Sox2 Level Is a Determinant of Cellular Reprogramming Potential |
title_fullStr | Sox2 Level Is a Determinant of Cellular Reprogramming Potential |
title_full_unstemmed | Sox2 Level Is a Determinant of Cellular Reprogramming Potential |
title_short | Sox2 Level Is a Determinant of Cellular Reprogramming Potential |
title_sort | sox2 level is a determinant of cellular reprogramming potential |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688988/ https://www.ncbi.nlm.nih.gov/pubmed/23825671 http://dx.doi.org/10.1371/journal.pone.0067594 |
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