A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics

Time-resolved quantitative analysis of auxin-mediated processes in plant cells is as of yet limited. By applying a synergistic mammalian and plant synthetic biology approach, we have developed a novel ratiometric luminescent biosensor with wide applicability in the study of auxin metabolism, transpo...

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Autores principales: Wend, Sabrina, Bosco, Cristina Dal, Kämpf, Michael M., Ren, Fugang, Palme, Klaus, Weber, Wilfried, Dovzhenko, Alexander, Zurbriggen, Matias D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689175/
https://www.ncbi.nlm.nih.gov/pubmed/23787479
http://dx.doi.org/10.1038/srep02052
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author Wend, Sabrina
Bosco, Cristina Dal
Kämpf, Michael M.
Ren, Fugang
Palme, Klaus
Weber, Wilfried
Dovzhenko, Alexander
Zurbriggen, Matias D.
author_facet Wend, Sabrina
Bosco, Cristina Dal
Kämpf, Michael M.
Ren, Fugang
Palme, Klaus
Weber, Wilfried
Dovzhenko, Alexander
Zurbriggen, Matias D.
author_sort Wend, Sabrina
collection PubMed
description Time-resolved quantitative analysis of auxin-mediated processes in plant cells is as of yet limited. By applying a synergistic mammalian and plant synthetic biology approach, we have developed a novel ratiometric luminescent biosensor with wide applicability in the study of auxin metabolism, transport, and signalling. The sensitivity and kinetic properties of our genetically encoded biosensor open new perspectives for the analysis of highly complex auxin dynamics in plant growth and development.
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spelling pubmed-36891752013-06-24 A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics Wend, Sabrina Bosco, Cristina Dal Kämpf, Michael M. Ren, Fugang Palme, Klaus Weber, Wilfried Dovzhenko, Alexander Zurbriggen, Matias D. Sci Rep Article Time-resolved quantitative analysis of auxin-mediated processes in plant cells is as of yet limited. By applying a synergistic mammalian and plant synthetic biology approach, we have developed a novel ratiometric luminescent biosensor with wide applicability in the study of auxin metabolism, transport, and signalling. The sensitivity and kinetic properties of our genetically encoded biosensor open new perspectives for the analysis of highly complex auxin dynamics in plant growth and development. Nature Publishing Group 2013-06-21 /pmc/articles/PMC3689175/ /pubmed/23787479 http://dx.doi.org/10.1038/srep02052 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Wend, Sabrina
Bosco, Cristina Dal
Kämpf, Michael M.
Ren, Fugang
Palme, Klaus
Weber, Wilfried
Dovzhenko, Alexander
Zurbriggen, Matias D.
A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics
title A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics
title_full A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics
title_fullStr A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics
title_full_unstemmed A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics
title_short A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics
title_sort quantitative ratiometric sensor for time-resolved analysis of auxin dynamics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689175/
https://www.ncbi.nlm.nih.gov/pubmed/23787479
http://dx.doi.org/10.1038/srep02052
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