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The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments
Most macromolecular crystallography (MX) diffraction experiments at synchrotrons use a single-axis goniometer. This markedly contrasts with small-molecule crystallography, in which the majority of the diffraction data are collected using multi-axis goniometers. A novel miniaturized κ-goniometer hea...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689527/ https://www.ncbi.nlm.nih.gov/pubmed/23793150 http://dx.doi.org/10.1107/S0907444913003880 |
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author | Brockhauser, Sandor Ravelli, Raimond B. G. McCarthy, Andrew A. |
author_facet | Brockhauser, Sandor Ravelli, Raimond B. G. McCarthy, Andrew A. |
author_sort | Brockhauser, Sandor |
collection | PubMed |
description | Most macromolecular crystallography (MX) diffraction experiments at synchrotrons use a single-axis goniometer. This markedly contrasts with small-molecule crystallography, in which the majority of the diffraction data are collected using multi-axis goniometers. A novel miniaturized κ-goniometer head, the MK3, has been developed to allow macromolecular crystals to be aligned. It is available on the majority of the structural biology beamlines at the ESRF, as well as elsewhere. In addition, the Strategy for the Alignment of Crystals (STAC) software package has been developed to facilitate the use of the MK3 and other similar devices. Use of the MK3 and STAC is streamlined by their incorporation into online analysis tools such as EDNA. The current use of STAC and MK3 on the MX beamlines at the ESRF is discussed. It is shown that the alignment of macromolecular crystals can result in improved diffraction data quality compared with data obtained from randomly aligned crystals. |
format | Online Article Text |
id | pubmed-3689527 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-36895272013-06-28 The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments Brockhauser, Sandor Ravelli, Raimond B. G. McCarthy, Andrew A. Acta Crystallogr D Biol Crystallogr Research Papers Most macromolecular crystallography (MX) diffraction experiments at synchrotrons use a single-axis goniometer. This markedly contrasts with small-molecule crystallography, in which the majority of the diffraction data are collected using multi-axis goniometers. A novel miniaturized κ-goniometer head, the MK3, has been developed to allow macromolecular crystals to be aligned. It is available on the majority of the structural biology beamlines at the ESRF, as well as elsewhere. In addition, the Strategy for the Alignment of Crystals (STAC) software package has been developed to facilitate the use of the MK3 and other similar devices. Use of the MK3 and STAC is streamlined by their incorporation into online analysis tools such as EDNA. The current use of STAC and MK3 on the MX beamlines at the ESRF is discussed. It is shown that the alignment of macromolecular crystals can result in improved diffraction data quality compared with data obtained from randomly aligned crystals. International Union of Crystallography 2013-06-15 /pmc/articles/PMC3689527/ /pubmed/23793150 http://dx.doi.org/10.1107/S0907444913003880 Text en © Brockhauser et al. 2013 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Brockhauser, Sandor Ravelli, Raimond B. G. McCarthy, Andrew A. The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments |
title | The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments |
title_full | The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments |
title_fullStr | The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments |
title_full_unstemmed | The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments |
title_short | The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments |
title_sort | use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689527/ https://www.ncbi.nlm.nih.gov/pubmed/23793150 http://dx.doi.org/10.1107/S0907444913003880 |
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