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Microcrystal manipulation with laser tweezers

X-ray crystallography is the method of choice to deduce atomic resolution structural information from macromolecules. In recent years, significant investments in structural genomics initiatives have been undertaken to automate all steps in X-ray crystallography from protein expression to structure s...

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Detalles Bibliográficos
Autores principales: Wagner, Armin, Duman, Ramona, Stevens, Bob, Ward, Andy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689533/
https://www.ncbi.nlm.nih.gov/pubmed/23793156
http://dx.doi.org/10.1107/S090744491300958X
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author Wagner, Armin
Duman, Ramona
Stevens, Bob
Ward, Andy
author_facet Wagner, Armin
Duman, Ramona
Stevens, Bob
Ward, Andy
author_sort Wagner, Armin
collection PubMed
description X-ray crystallography is the method of choice to deduce atomic resolution structural information from macromolecules. In recent years, significant investments in structural genomics initiatives have been undertaken to automate all steps in X-ray crystallography from protein expression to structure solution. Robotic systems are widely used to prepare crystallization screens and change samples on synchrotron beamlines for macromolecular crystallography. The only remaining manual handling step is the transfer of the crystal from the mother liquor onto the crystal holder. Manual mounting is relatively straightforward for crystals with dimensions of >25 µm; however, this step is nontrivial for smaller crystals. The mounting of microcrystals is becoming increasingly important as advances in microfocus synchrotron beamlines now allow data collection from crystals with dimensions of only a few micrometres. To make optimal usage of these beamlines, new approaches have to be taken to facilitate and automate this last manual handling step. Optical tweezers, which are routinely used for the manipulation of micrometre-sized objects, have successfully been applied to sort and mount macromolecular crystals on newly designed crystal holders. Diffraction data from CPV type 1 polyhedrin microcrystals mounted with laser tweezers are presented.
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spelling pubmed-36895332013-06-28 Microcrystal manipulation with laser tweezers Wagner, Armin Duman, Ramona Stevens, Bob Ward, Andy Acta Crystallogr D Biol Crystallogr Research Papers X-ray crystallography is the method of choice to deduce atomic resolution structural information from macromolecules. In recent years, significant investments in structural genomics initiatives have been undertaken to automate all steps in X-ray crystallography from protein expression to structure solution. Robotic systems are widely used to prepare crystallization screens and change samples on synchrotron beamlines for macromolecular crystallography. The only remaining manual handling step is the transfer of the crystal from the mother liquor onto the crystal holder. Manual mounting is relatively straightforward for crystals with dimensions of >25 µm; however, this step is nontrivial for smaller crystals. The mounting of microcrystals is becoming increasingly important as advances in microfocus synchrotron beamlines now allow data collection from crystals with dimensions of only a few micrometres. To make optimal usage of these beamlines, new approaches have to be taken to facilitate and automate this last manual handling step. Optical tweezers, which are routinely used for the manipulation of micrometre-sized objects, have successfully been applied to sort and mount macromolecular crystals on newly designed crystal holders. Diffraction data from CPV type 1 polyhedrin microcrystals mounted with laser tweezers are presented. International Union of Crystallography 2013-07-01 2013-06-13 /pmc/articles/PMC3689533/ /pubmed/23793156 http://dx.doi.org/10.1107/S090744491300958X Text en © Wagner et al. 2013 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Wagner, Armin
Duman, Ramona
Stevens, Bob
Ward, Andy
Microcrystal manipulation with laser tweezers
title Microcrystal manipulation with laser tweezers
title_full Microcrystal manipulation with laser tweezers
title_fullStr Microcrystal manipulation with laser tweezers
title_full_unstemmed Microcrystal manipulation with laser tweezers
title_short Microcrystal manipulation with laser tweezers
title_sort microcrystal manipulation with laser tweezers
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689533/
https://www.ncbi.nlm.nih.gov/pubmed/23793156
http://dx.doi.org/10.1107/S090744491300958X
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