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Microcrystal manipulation with laser tweezers
X-ray crystallography is the method of choice to deduce atomic resolution structural information from macromolecules. In recent years, significant investments in structural genomics initiatives have been undertaken to automate all steps in X-ray crystallography from protein expression to structure s...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689533/ https://www.ncbi.nlm.nih.gov/pubmed/23793156 http://dx.doi.org/10.1107/S090744491300958X |
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author | Wagner, Armin Duman, Ramona Stevens, Bob Ward, Andy |
author_facet | Wagner, Armin Duman, Ramona Stevens, Bob Ward, Andy |
author_sort | Wagner, Armin |
collection | PubMed |
description | X-ray crystallography is the method of choice to deduce atomic resolution structural information from macromolecules. In recent years, significant investments in structural genomics initiatives have been undertaken to automate all steps in X-ray crystallography from protein expression to structure solution. Robotic systems are widely used to prepare crystallization screens and change samples on synchrotron beamlines for macromolecular crystallography. The only remaining manual handling step is the transfer of the crystal from the mother liquor onto the crystal holder. Manual mounting is relatively straightforward for crystals with dimensions of >25 µm; however, this step is nontrivial for smaller crystals. The mounting of microcrystals is becoming increasingly important as advances in microfocus synchrotron beamlines now allow data collection from crystals with dimensions of only a few micrometres. To make optimal usage of these beamlines, new approaches have to be taken to facilitate and automate this last manual handling step. Optical tweezers, which are routinely used for the manipulation of micrometre-sized objects, have successfully been applied to sort and mount macromolecular crystals on newly designed crystal holders. Diffraction data from CPV type 1 polyhedrin microcrystals mounted with laser tweezers are presented. |
format | Online Article Text |
id | pubmed-3689533 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-36895332013-06-28 Microcrystal manipulation with laser tweezers Wagner, Armin Duman, Ramona Stevens, Bob Ward, Andy Acta Crystallogr D Biol Crystallogr Research Papers X-ray crystallography is the method of choice to deduce atomic resolution structural information from macromolecules. In recent years, significant investments in structural genomics initiatives have been undertaken to automate all steps in X-ray crystallography from protein expression to structure solution. Robotic systems are widely used to prepare crystallization screens and change samples on synchrotron beamlines for macromolecular crystallography. The only remaining manual handling step is the transfer of the crystal from the mother liquor onto the crystal holder. Manual mounting is relatively straightforward for crystals with dimensions of >25 µm; however, this step is nontrivial for smaller crystals. The mounting of microcrystals is becoming increasingly important as advances in microfocus synchrotron beamlines now allow data collection from crystals with dimensions of only a few micrometres. To make optimal usage of these beamlines, new approaches have to be taken to facilitate and automate this last manual handling step. Optical tweezers, which are routinely used for the manipulation of micrometre-sized objects, have successfully been applied to sort and mount macromolecular crystals on newly designed crystal holders. Diffraction data from CPV type 1 polyhedrin microcrystals mounted with laser tweezers are presented. International Union of Crystallography 2013-07-01 2013-06-13 /pmc/articles/PMC3689533/ /pubmed/23793156 http://dx.doi.org/10.1107/S090744491300958X Text en © Wagner et al. 2013 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Wagner, Armin Duman, Ramona Stevens, Bob Ward, Andy Microcrystal manipulation with laser tweezers |
title | Microcrystal manipulation with laser tweezers |
title_full | Microcrystal manipulation with laser tweezers |
title_fullStr | Microcrystal manipulation with laser tweezers |
title_full_unstemmed | Microcrystal manipulation with laser tweezers |
title_short | Microcrystal manipulation with laser tweezers |
title_sort | microcrystal manipulation with laser tweezers |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689533/ https://www.ncbi.nlm.nih.gov/pubmed/23793156 http://dx.doi.org/10.1107/S090744491300958X |
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