c-Abl Kinase Is a Regulator of α(v)β(3) Integrin Mediated Melanoma A375 Cell Migration

Integrins are heterodimeric transmembrane receptors that physically link the extracellular matrix (ECM) to the intracellular actin cytoskeleton, and are also signaling molecules that transduce signals bi-directionally across the plasma membrane. Integrin regulation is essential for tumor cell migration in response to growth factors. c-Abl kinase is a nonreceptor tyrosine kinase and is critical for signaling transduction from various receptors. Here we show that c-Abl kinase is involved in A375 cell migration mediated by α(v)β(3) integrin in response to PDGF stimulation. c-Abl kinase colocalizes with α(v)β(3) integrin dynamically and affects α(v)β(3) integrin affinity by regulating its cluster. The interaction between c-Abl kinase and α(v)β(3) integrin was dependent on the activity of c-Abl kinase induced by PDGF stimulation, but was not dependent on the binding of α(v)β(3) integrin with its ligands, suggesting that c-Abl kinase is not involved in the outside-in signaling of α(v)β(3) integrin. Talin head domain was required for the interaction between c-Abl kinase and α(v)β(3) integrin, and the SH3 domain of c-Abl kinase was involved in its interaction with talin and α(v)β(3) integrin. Taken together, we have uncovered a novel and critical role of c-Abl kinase in α(v)β(3) integrin mediated melanoma cell migration.