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Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE
Besides the classical form of bovine spongiform encephalopathy (BSE) that has been known for almost three decades, two atypical forms designated H-type and L-type BSE have recently been described. While the main diagnostic feature of these forms is the altered biochemical profile of the accumulated...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689710/ https://www.ncbi.nlm.nih.gov/pubmed/23805320 http://dx.doi.org/10.1371/journal.pone.0067599 |
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author | Priemer, Grit Balkema-Buschmann, Anne Hills, Bob Groschup, Martin H. |
author_facet | Priemer, Grit Balkema-Buschmann, Anne Hills, Bob Groschup, Martin H. |
author_sort | Priemer, Grit |
collection | PubMed |
description | Besides the classical form of bovine spongiform encephalopathy (BSE) that has been known for almost three decades, two atypical forms designated H-type and L-type BSE have recently been described. While the main diagnostic feature of these forms is the altered biochemical profile of the accumulated PrP(Sc), it was also observed in the initial analysis that L-type BSE displays a distribution pattern of the pathological prion protein (PrP(Sc)), which clearly differs from that observed in classical BSE (C-type). Most importantly, the obex region in the brainstem is not the region with the highest PrP(Sc) concentrations, but PrP(Sc) is spread more evenly throughout the entire brain. A similar distribution pattern has been revealed for H-type BSE by rapid test analysis. Based on these findings, we performed a more detailed Western blot study of the anatomical PrP(Sc) distribution pattern and the biochemical characteristics (molecular mass, glycoprofile as well as PK sensitivity) in ten different anatomical locations of the brain from cattle experimentally challenged with H- or L-type BSE, as compared to cattle challenged with C-type BSE. Results of this study revealed distinct differences in the PrP(Sc) deposition patterns between all three BSE forms, while the biochemical characteristics remained stable for each BSE type among all analysed brain areas. |
format | Online Article Text |
id | pubmed-3689710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36897102013-06-26 Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE Priemer, Grit Balkema-Buschmann, Anne Hills, Bob Groschup, Martin H. PLoS One Research Article Besides the classical form of bovine spongiform encephalopathy (BSE) that has been known for almost three decades, two atypical forms designated H-type and L-type BSE have recently been described. While the main diagnostic feature of these forms is the altered biochemical profile of the accumulated PrP(Sc), it was also observed in the initial analysis that L-type BSE displays a distribution pattern of the pathological prion protein (PrP(Sc)), which clearly differs from that observed in classical BSE (C-type). Most importantly, the obex region in the brainstem is not the region with the highest PrP(Sc) concentrations, but PrP(Sc) is spread more evenly throughout the entire brain. A similar distribution pattern has been revealed for H-type BSE by rapid test analysis. Based on these findings, we performed a more detailed Western blot study of the anatomical PrP(Sc) distribution pattern and the biochemical characteristics (molecular mass, glycoprofile as well as PK sensitivity) in ten different anatomical locations of the brain from cattle experimentally challenged with H- or L-type BSE, as compared to cattle challenged with C-type BSE. Results of this study revealed distinct differences in the PrP(Sc) deposition patterns between all three BSE forms, while the biochemical characteristics remained stable for each BSE type among all analysed brain areas. Public Library of Science 2013-06-21 /pmc/articles/PMC3689710/ /pubmed/23805320 http://dx.doi.org/10.1371/journal.pone.0067599 Text en © 2013 Priemer et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Priemer, Grit Balkema-Buschmann, Anne Hills, Bob Groschup, Martin H. Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE |
title | Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE |
title_full | Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE |
title_fullStr | Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE |
title_full_unstemmed | Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE |
title_short | Biochemical Characteristics and PrP(Sc) Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE |
title_sort | biochemical characteristics and prp(sc) distribution pattern in the brains of cattle experimentally challenged with h-type and l-type atypical bse |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689710/ https://www.ncbi.nlm.nih.gov/pubmed/23805320 http://dx.doi.org/10.1371/journal.pone.0067599 |
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