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A Nucleosomal Region Important for Ensuring Proper Interactions Between the Transcription Elongation Factor Spt16 and Transcribed Genes in Saccharomyces cerevisiae

The highly conserved FACT (FAcilitates Chromatin Transactions) histone chaperone assists in the transcription elongation process first by facilitating the removal of histones in front of transcribing RNA polymerase II (Pol II) and then by contributing to nucleosome reassembly in the wake of Pol II p...

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Autores principales: Nguyen, Hoai-Trang T., Wharton, William, Harper, Jennifer A., Dornhoffer, James R., Duina, Andrea A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689804/
https://www.ncbi.nlm.nih.gov/pubmed/23576521
http://dx.doi.org/10.1534/g3.113.005926
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author Nguyen, Hoai-Trang T.
Wharton, William
Harper, Jennifer A.
Dornhoffer, James R.
Duina, Andrea A.
author_facet Nguyen, Hoai-Trang T.
Wharton, William
Harper, Jennifer A.
Dornhoffer, James R.
Duina, Andrea A.
author_sort Nguyen, Hoai-Trang T.
collection PubMed
description The highly conserved FACT (FAcilitates Chromatin Transactions) histone chaperone assists in the transcription elongation process first by facilitating the removal of histones in front of transcribing RNA polymerase II (Pol II) and then by contributing to nucleosome reassembly in the wake of Pol II passage. Whereas it is well established that FACT localizes across actively transcribed genes, the mechanisms that regulate FACT recruitment to and disengagement from chromatin during transcription still remain to be elucidated. Using the Saccharomyces cerevisiae model system, we previously showed that a histone H3 mutant—H3-L61W—greatly perturbs interactions between the yeast FACT (yFACT) complex and chromatin during transcription, resulting in a pronounced shift in yFACT occupancy toward the 3′ ends of transcribed genes. In the present study we report that two histone H4 mutants—H4-R36A and H4-K31E—alter the association pattern of the yFACT subunit Spt16 across transcribed genes in a fashion similar to that seen for H3-L61W. Interestingly, H4-R36, H4-K31, and H3-L61 are in close proximity to each other on the side of the nucleosome. We also provide evidence that the H4-R36A and H3-L61W mutants impair proper Spt16−chromatin interactions by perturbing a common process. Collectively, our results suggest that a nucleosomal region encompassing the H4-R36, H4-K31, and H3-L61 residues plays an important role in ensuring proper association of yFACT across transcribed genes.
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spelling pubmed-36898042013-06-24 A Nucleosomal Region Important for Ensuring Proper Interactions Between the Transcription Elongation Factor Spt16 and Transcribed Genes in Saccharomyces cerevisiae Nguyen, Hoai-Trang T. Wharton, William Harper, Jennifer A. Dornhoffer, James R. Duina, Andrea A. G3 (Bethesda) Investigations The highly conserved FACT (FAcilitates Chromatin Transactions) histone chaperone assists in the transcription elongation process first by facilitating the removal of histones in front of transcribing RNA polymerase II (Pol II) and then by contributing to nucleosome reassembly in the wake of Pol II passage. Whereas it is well established that FACT localizes across actively transcribed genes, the mechanisms that regulate FACT recruitment to and disengagement from chromatin during transcription still remain to be elucidated. Using the Saccharomyces cerevisiae model system, we previously showed that a histone H3 mutant—H3-L61W—greatly perturbs interactions between the yeast FACT (yFACT) complex and chromatin during transcription, resulting in a pronounced shift in yFACT occupancy toward the 3′ ends of transcribed genes. In the present study we report that two histone H4 mutants—H4-R36A and H4-K31E—alter the association pattern of the yFACT subunit Spt16 across transcribed genes in a fashion similar to that seen for H3-L61W. Interestingly, H4-R36, H4-K31, and H3-L61 are in close proximity to each other on the side of the nucleosome. We also provide evidence that the H4-R36A and H3-L61W mutants impair proper Spt16−chromatin interactions by perturbing a common process. Collectively, our results suggest that a nucleosomal region encompassing the H4-R36, H4-K31, and H3-L61 residues plays an important role in ensuring proper association of yFACT across transcribed genes. Genetics Society of America 2013-06-01 /pmc/articles/PMC3689804/ /pubmed/23576521 http://dx.doi.org/10.1534/g3.113.005926 Text en Copyright © 2013 Nguyen et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Nguyen, Hoai-Trang T.
Wharton, William
Harper, Jennifer A.
Dornhoffer, James R.
Duina, Andrea A.
A Nucleosomal Region Important for Ensuring Proper Interactions Between the Transcription Elongation Factor Spt16 and Transcribed Genes in Saccharomyces cerevisiae
title A Nucleosomal Region Important for Ensuring Proper Interactions Between the Transcription Elongation Factor Spt16 and Transcribed Genes in Saccharomyces cerevisiae
title_full A Nucleosomal Region Important for Ensuring Proper Interactions Between the Transcription Elongation Factor Spt16 and Transcribed Genes in Saccharomyces cerevisiae
title_fullStr A Nucleosomal Region Important for Ensuring Proper Interactions Between the Transcription Elongation Factor Spt16 and Transcribed Genes in Saccharomyces cerevisiae
title_full_unstemmed A Nucleosomal Region Important for Ensuring Proper Interactions Between the Transcription Elongation Factor Spt16 and Transcribed Genes in Saccharomyces cerevisiae
title_short A Nucleosomal Region Important for Ensuring Proper Interactions Between the Transcription Elongation Factor Spt16 and Transcribed Genes in Saccharomyces cerevisiae
title_sort nucleosomal region important for ensuring proper interactions between the transcription elongation factor spt16 and transcribed genes in saccharomyces cerevisiae
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689804/
https://www.ncbi.nlm.nih.gov/pubmed/23576521
http://dx.doi.org/10.1534/g3.113.005926
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