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Dual-Specific Interaction to Detect DNA on Gold Nanoparticles
An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690027/ https://www.ncbi.nlm.nih.gov/pubmed/23645111 http://dx.doi.org/10.3390/s130505749 |
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author | Feng, Chuan-Liang Dou, Xiao-Qiu Liu, Qing-Lei Zhang, Wang Gu, Jia-Jun Zhu, Shen-Min Jenkins, Andrew Tobias Aveling Zhang, Di |
author_facet | Feng, Chuan-Liang Dou, Xiao-Qiu Liu, Qing-Lei Zhang, Wang Gu, Jia-Jun Zhu, Shen-Min Jenkins, Andrew Tobias Aveling Zhang, Di |
author_sort | Feng, Chuan-Liang |
collection | PubMed |
description | An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as “bridges” to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules. |
format | Online Article Text |
id | pubmed-3690027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-36900272013-07-09 Dual-Specific Interaction to Detect DNA on Gold Nanoparticles Feng, Chuan-Liang Dou, Xiao-Qiu Liu, Qing-Lei Zhang, Wang Gu, Jia-Jun Zhu, Shen-Min Jenkins, Andrew Tobias Aveling Zhang, Di Sensors (Basel) Article An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as “bridges” to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules. Molecular Diversity Preservation International (MDPI) 2013-05-03 /pmc/articles/PMC3690027/ /pubmed/23645111 http://dx.doi.org/10.3390/s130505749 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Article Feng, Chuan-Liang Dou, Xiao-Qiu Liu, Qing-Lei Zhang, Wang Gu, Jia-Jun Zhu, Shen-Min Jenkins, Andrew Tobias Aveling Zhang, Di Dual-Specific Interaction to Detect DNA on Gold Nanoparticles |
title | Dual-Specific Interaction to Detect DNA on Gold Nanoparticles |
title_full | Dual-Specific Interaction to Detect DNA on Gold Nanoparticles |
title_fullStr | Dual-Specific Interaction to Detect DNA on Gold Nanoparticles |
title_full_unstemmed | Dual-Specific Interaction to Detect DNA on Gold Nanoparticles |
title_short | Dual-Specific Interaction to Detect DNA on Gold Nanoparticles |
title_sort | dual-specific interaction to detect dna on gold nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690027/ https://www.ncbi.nlm.nih.gov/pubmed/23645111 http://dx.doi.org/10.3390/s130505749 |
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