Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery

Whole-cell biosensors are a good alternative to enzyme-based biosensors since they offer the benefits of low cost and improved stability. In recent years, live cells have been employed as biosensors for a wide range of targets. In this review, we will focus on the use of microorganisms that are gene...

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Detalles Bibliográficos
Autores principales: Park, Miso, Tsai, Shen-Long, Chen, Wilfred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690029/
https://www.ncbi.nlm.nih.gov/pubmed/23648649
http://dx.doi.org/10.3390/s130505777
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author Park, Miso
Tsai, Shen-Long
Chen, Wilfred
author_facet Park, Miso
Tsai, Shen-Long
Chen, Wilfred
author_sort Park, Miso
collection PubMed
description Whole-cell biosensors are a good alternative to enzyme-based biosensors since they offer the benefits of low cost and improved stability. In recent years, live cells have been employed as biosensors for a wide range of targets. In this review, we will focus on the use of microorganisms that are genetically modified with the desirable outputs in order to improve the biosensor performance. Different methodologies based on genetic/protein engineering and synthetic biology to construct microorganisms with the required signal outputs, sensitivity, and selectivity will be discussed.
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spelling pubmed-36900292013-07-09 Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery Park, Miso Tsai, Shen-Long Chen, Wilfred Sensors (Basel) Review Whole-cell biosensors are a good alternative to enzyme-based biosensors since they offer the benefits of low cost and improved stability. In recent years, live cells have been employed as biosensors for a wide range of targets. In this review, we will focus on the use of microorganisms that are genetically modified with the desirable outputs in order to improve the biosensor performance. Different methodologies based on genetic/protein engineering and synthetic biology to construct microorganisms with the required signal outputs, sensitivity, and selectivity will be discussed. Molecular Diversity Preservation International (MDPI) 2013-05-06 /pmc/articles/PMC3690029/ /pubmed/23648649 http://dx.doi.org/10.3390/s130505777 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/
spellingShingle Review
Park, Miso
Tsai, Shen-Long
Chen, Wilfred
Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery
title Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery
title_full Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery
title_fullStr Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery
title_full_unstemmed Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery
title_short Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery
title_sort microbial biosensors: engineered microorganisms as the sensing machinery
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690029/
https://www.ncbi.nlm.nih.gov/pubmed/23648649
http://dx.doi.org/10.3390/s130505777
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AT tsaishenlong microbialbiosensorsengineeredmicroorganismsasthesensingmachinery
AT chenwilfred microbialbiosensorsengineeredmicroorganismsasthesensingmachinery

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