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Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants

Fluorescent protein (FP) tagging approaches are widely used to determine the subcellular location of plant proteins. Here we give a brief overview of FP approaches, highlight potential technical problems, and discuss what to consider when designing FP/protein fusion constructs and performing transfo...

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Autores principales: Tanz, Sandra K., Castleden, Ian, Small, Ian D., Millar, A. Harvey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690342/
https://www.ncbi.nlm.nih.gov/pubmed/23805149
http://dx.doi.org/10.3389/fpls.2013.00214
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author Tanz, Sandra K.
Castleden, Ian
Small, Ian D.
Millar, A. Harvey
author_facet Tanz, Sandra K.
Castleden, Ian
Small, Ian D.
Millar, A. Harvey
author_sort Tanz, Sandra K.
collection PubMed
description Fluorescent protein (FP) tagging approaches are widely used to determine the subcellular location of plant proteins. Here we give a brief overview of FP approaches, highlight potential technical problems, and discuss what to consider when designing FP/protein fusion constructs and performing transformation assays. We analyze published FP tagging data sets along with data from proteomics studies collated in SUBA3, a subcellular location database for Arabidopsis proteins, and assess the reliability of these data sets by comparing them. We also outline the limitations of the FP tagging approach for defining protein location and investigate multiple localization claims by FP tagging. We conclude that the collation of localization datasets in databases like SUBA3 is helpful for revealing discrepancies in location attributions by different techniques and/or by different research groups.
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spelling pubmed-36903422013-06-26 Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants Tanz, Sandra K. Castleden, Ian Small, Ian D. Millar, A. Harvey Front Plant Sci Plant Science Fluorescent protein (FP) tagging approaches are widely used to determine the subcellular location of plant proteins. Here we give a brief overview of FP approaches, highlight potential technical problems, and discuss what to consider when designing FP/protein fusion constructs and performing transformation assays. We analyze published FP tagging data sets along with data from proteomics studies collated in SUBA3, a subcellular location database for Arabidopsis proteins, and assess the reliability of these data sets by comparing them. We also outline the limitations of the FP tagging approach for defining protein location and investigate multiple localization claims by FP tagging. We conclude that the collation of localization datasets in databases like SUBA3 is helpful for revealing discrepancies in location attributions by different techniques and/or by different research groups. Frontiers Media S.A. 2013-06-24 /pmc/articles/PMC3690342/ /pubmed/23805149 http://dx.doi.org/10.3389/fpls.2013.00214 Text en Copyright © 2013 Tanz, Castleden, Small and Millar. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
spellingShingle Plant Science
Tanz, Sandra K.
Castleden, Ian
Small, Ian D.
Millar, A. Harvey
Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants
title Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants
title_full Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants
title_fullStr Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants
title_full_unstemmed Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants
title_short Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants
title_sort fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690342/
https://www.ncbi.nlm.nih.gov/pubmed/23805149
http://dx.doi.org/10.3389/fpls.2013.00214
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