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Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants

Fluorescent protein (FP) tagging approaches are widely used to determine the subcellular location of plant proteins. Here we give a brief overview of FP approaches, highlight potential technical problems, and discuss what to consider when designing FP/protein fusion constructs and performing transfo...

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Detalles Bibliográficos
Autores principales: Tanz, Sandra K., Castleden, Ian, Small, Ian D., Millar, A. Harvey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690342/
https://www.ncbi.nlm.nih.gov/pubmed/23805149
http://dx.doi.org/10.3389/fpls.2013.00214