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Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants
Fluorescent protein (FP) tagging approaches are widely used to determine the subcellular location of plant proteins. Here we give a brief overview of FP approaches, highlight potential technical problems, and discuss what to consider when designing FP/protein fusion constructs and performing transfo...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690342/ https://www.ncbi.nlm.nih.gov/pubmed/23805149 http://dx.doi.org/10.3389/fpls.2013.00214 |