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Preliminary Assessment of Microbiome Changes Following Blood-Feeding and Survivorship in the Amblyomma americanum Nymph-to-Adult Transition using Semiconductor Sequencing

The physiology of ticks supports a diverse community of non-pathogenic and pathogenic organisms. This study aims to initially characterize the microbial community present within colony-reared Amblyomma americanum using PCR of the variable region 5 of the 16S rRNA gene followed by semiconductor seque...

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Autores principales: Menchaca, Arturo C., Visi, David K., Strey, Otto F., Teel, Pete D., Kalinowski, Kevin, Allen, Michael S., Williamson, Phillip C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691118/
https://www.ncbi.nlm.nih.gov/pubmed/23826210
http://dx.doi.org/10.1371/journal.pone.0067129
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author Menchaca, Arturo C.
Visi, David K.
Strey, Otto F.
Teel, Pete D.
Kalinowski, Kevin
Allen, Michael S.
Williamson, Phillip C.
author_facet Menchaca, Arturo C.
Visi, David K.
Strey, Otto F.
Teel, Pete D.
Kalinowski, Kevin
Allen, Michael S.
Williamson, Phillip C.
author_sort Menchaca, Arturo C.
collection PubMed
description The physiology of ticks supports a diverse community of non-pathogenic and pathogenic organisms. This study aims to initially characterize the microbial community present within colony-reared Amblyomma americanum using PCR of the variable region 5 of the 16S rRNA gene followed by semiconductor sequencing and classification of sequence data using the Ribosomal Database Project and MG-RAST analysis tools. Comparison of amplicon library datasets revealed changes in the microbiomes in newly engorged nymphs, newly-molted adults, and aged adults, as well as ticks exposed to different environmental conditions. These preliminary data support the concept that microbe survivorship and diversity are partially dependent upon environmental variables and the sequence of blood feeding, molting, and aging. The maintenance and/or emergence of pathogens in ticks may be dependent in part on temporal changes in the microbial community of the tick microbiome.
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spelling pubmed-36911182013-07-03 Preliminary Assessment of Microbiome Changes Following Blood-Feeding and Survivorship in the Amblyomma americanum Nymph-to-Adult Transition using Semiconductor Sequencing Menchaca, Arturo C. Visi, David K. Strey, Otto F. Teel, Pete D. Kalinowski, Kevin Allen, Michael S. Williamson, Phillip C. PLoS One Research Article The physiology of ticks supports a diverse community of non-pathogenic and pathogenic organisms. This study aims to initially characterize the microbial community present within colony-reared Amblyomma americanum using PCR of the variable region 5 of the 16S rRNA gene followed by semiconductor sequencing and classification of sequence data using the Ribosomal Database Project and MG-RAST analysis tools. Comparison of amplicon library datasets revealed changes in the microbiomes in newly engorged nymphs, newly-molted adults, and aged adults, as well as ticks exposed to different environmental conditions. These preliminary data support the concept that microbe survivorship and diversity are partially dependent upon environmental variables and the sequence of blood feeding, molting, and aging. The maintenance and/or emergence of pathogens in ticks may be dependent in part on temporal changes in the microbial community of the tick microbiome. Public Library of Science 2013-06-24 /pmc/articles/PMC3691118/ /pubmed/23826210 http://dx.doi.org/10.1371/journal.pone.0067129 Text en © 2013 Menchaca et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Menchaca, Arturo C.
Visi, David K.
Strey, Otto F.
Teel, Pete D.
Kalinowski, Kevin
Allen, Michael S.
Williamson, Phillip C.
Preliminary Assessment of Microbiome Changes Following Blood-Feeding and Survivorship in the Amblyomma americanum Nymph-to-Adult Transition using Semiconductor Sequencing
title Preliminary Assessment of Microbiome Changes Following Blood-Feeding and Survivorship in the Amblyomma americanum Nymph-to-Adult Transition using Semiconductor Sequencing
title_full Preliminary Assessment of Microbiome Changes Following Blood-Feeding and Survivorship in the Amblyomma americanum Nymph-to-Adult Transition using Semiconductor Sequencing
title_fullStr Preliminary Assessment of Microbiome Changes Following Blood-Feeding and Survivorship in the Amblyomma americanum Nymph-to-Adult Transition using Semiconductor Sequencing
title_full_unstemmed Preliminary Assessment of Microbiome Changes Following Blood-Feeding and Survivorship in the Amblyomma americanum Nymph-to-Adult Transition using Semiconductor Sequencing
title_short Preliminary Assessment of Microbiome Changes Following Blood-Feeding and Survivorship in the Amblyomma americanum Nymph-to-Adult Transition using Semiconductor Sequencing
title_sort preliminary assessment of microbiome changes following blood-feeding and survivorship in the amblyomma americanum nymph-to-adult transition using semiconductor sequencing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691118/
https://www.ncbi.nlm.nih.gov/pubmed/23826210
http://dx.doi.org/10.1371/journal.pone.0067129
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