Cargando…

First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children

Platelets are subcellular fragments which circulate in blood and have well established roles in thrombosis and haemostasis in adults. Upon activation, platelets undergo granule exocytosis and express P-Selectin on the cell membrane which binds a ligand on monocytes, leading to monocyte-platelet aggr...

Descripción completa

Detalles Bibliográficos
Autores principales: Yip, Christina, Ignjatovic, Vera, Attard, Chantal, Monagle, Paul, Linden, Matthew D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691142/
https://www.ncbi.nlm.nih.gov/pubmed/23826296
http://dx.doi.org/10.1371/journal.pone.0067416
_version_ 1782274422512549888
author Yip, Christina
Ignjatovic, Vera
Attard, Chantal
Monagle, Paul
Linden, Matthew D.
author_facet Yip, Christina
Ignjatovic, Vera
Attard, Chantal
Monagle, Paul
Linden, Matthew D.
author_sort Yip, Christina
collection PubMed
description Platelets are subcellular fragments which circulate in blood and have well established roles in thrombosis and haemostasis in adults. Upon activation, platelets undergo granule exocytosis and express P-Selectin on the cell membrane which binds a ligand on monocytes, leading to monocyte-platelet aggregation. Elevated circulating monocyte-platelet aggregates in adults are linked to atherothrombosis, but have not been investigated in children where thrombosis is less common. This study aimed to measure monocyte-platelet aggregate formation in children using whole blood flow cytometry. Monocyte-platelet aggregates as well as activation and granule exocytosis of platelets were measured in healthy adults (n = 15, median age 28 years) and healthy children (n = 28, median age 7 years). Monocyte-platelet aggregates in healthy children were elevated compared to healthy adults (37.8±4.4% vs 15.5±1.9% respectively, p<0.01). However, this was not accompanied by any difference in platelet activation (PAC-1 binding 6.8±1.5% vs 6.3±2.0% respectively, p = ns) or granule exocytosis (P-selectin expression 4.4±0.5% vs 3.1±0.5% respectively, p = ns). Despite comparable numbers of platelets bound per monocyte (GPIb MFI 117.3±13.7 vs 130.9±28.6 respectively, p = ns), surface P-selectin expression per platelet-bound monocyte was lower in children compared to adults. We therefore provide the first data of elevated monocyte-platelet aggregates in healthy children.
format Online
Article
Text
id pubmed-3691142
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-36911422013-07-03 First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children Yip, Christina Ignjatovic, Vera Attard, Chantal Monagle, Paul Linden, Matthew D. PLoS One Research Article Platelets are subcellular fragments which circulate in blood and have well established roles in thrombosis and haemostasis in adults. Upon activation, platelets undergo granule exocytosis and express P-Selectin on the cell membrane which binds a ligand on monocytes, leading to monocyte-platelet aggregation. Elevated circulating monocyte-platelet aggregates in adults are linked to atherothrombosis, but have not been investigated in children where thrombosis is less common. This study aimed to measure monocyte-platelet aggregate formation in children using whole blood flow cytometry. Monocyte-platelet aggregates as well as activation and granule exocytosis of platelets were measured in healthy adults (n = 15, median age 28 years) and healthy children (n = 28, median age 7 years). Monocyte-platelet aggregates in healthy children were elevated compared to healthy adults (37.8±4.4% vs 15.5±1.9% respectively, p<0.01). However, this was not accompanied by any difference in platelet activation (PAC-1 binding 6.8±1.5% vs 6.3±2.0% respectively, p = ns) or granule exocytosis (P-selectin expression 4.4±0.5% vs 3.1±0.5% respectively, p = ns). Despite comparable numbers of platelets bound per monocyte (GPIb MFI 117.3±13.7 vs 130.9±28.6 respectively, p = ns), surface P-selectin expression per platelet-bound monocyte was lower in children compared to adults. We therefore provide the first data of elevated monocyte-platelet aggregates in healthy children. Public Library of Science 2013-06-24 /pmc/articles/PMC3691142/ /pubmed/23826296 http://dx.doi.org/10.1371/journal.pone.0067416 Text en © 2013 Yip et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yip, Christina
Ignjatovic, Vera
Attard, Chantal
Monagle, Paul
Linden, Matthew D.
First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children
title First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children
title_full First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children
title_fullStr First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children
title_full_unstemmed First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children
title_short First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children
title_sort first report of elevated monocyte-platelet aggregates in healthy children
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691142/
https://www.ncbi.nlm.nih.gov/pubmed/23826296
http://dx.doi.org/10.1371/journal.pone.0067416
work_keys_str_mv AT yipchristina firstreportofelevatedmonocyteplateletaggregatesinhealthychildren
AT ignjatovicvera firstreportofelevatedmonocyteplateletaggregatesinhealthychildren
AT attardchantal firstreportofelevatedmonocyteplateletaggregatesinhealthychildren
AT monaglepaul firstreportofelevatedmonocyteplateletaggregatesinhealthychildren
AT lindenmatthewd firstreportofelevatedmonocyteplateletaggregatesinhealthychildren