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First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children
Platelets are subcellular fragments which circulate in blood and have well established roles in thrombosis and haemostasis in adults. Upon activation, platelets undergo granule exocytosis and express P-Selectin on the cell membrane which binds a ligand on monocytes, leading to monocyte-platelet aggr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691142/ https://www.ncbi.nlm.nih.gov/pubmed/23826296 http://dx.doi.org/10.1371/journal.pone.0067416 |
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author | Yip, Christina Ignjatovic, Vera Attard, Chantal Monagle, Paul Linden, Matthew D. |
author_facet | Yip, Christina Ignjatovic, Vera Attard, Chantal Monagle, Paul Linden, Matthew D. |
author_sort | Yip, Christina |
collection | PubMed |
description | Platelets are subcellular fragments which circulate in blood and have well established roles in thrombosis and haemostasis in adults. Upon activation, platelets undergo granule exocytosis and express P-Selectin on the cell membrane which binds a ligand on monocytes, leading to monocyte-platelet aggregation. Elevated circulating monocyte-platelet aggregates in adults are linked to atherothrombosis, but have not been investigated in children where thrombosis is less common. This study aimed to measure monocyte-platelet aggregate formation in children using whole blood flow cytometry. Monocyte-platelet aggregates as well as activation and granule exocytosis of platelets were measured in healthy adults (n = 15, median age 28 years) and healthy children (n = 28, median age 7 years). Monocyte-platelet aggregates in healthy children were elevated compared to healthy adults (37.8±4.4% vs 15.5±1.9% respectively, p<0.01). However, this was not accompanied by any difference in platelet activation (PAC-1 binding 6.8±1.5% vs 6.3±2.0% respectively, p = ns) or granule exocytosis (P-selectin expression 4.4±0.5% vs 3.1±0.5% respectively, p = ns). Despite comparable numbers of platelets bound per monocyte (GPIb MFI 117.3±13.7 vs 130.9±28.6 respectively, p = ns), surface P-selectin expression per platelet-bound monocyte was lower in children compared to adults. We therefore provide the first data of elevated monocyte-platelet aggregates in healthy children. |
format | Online Article Text |
id | pubmed-3691142 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36911422013-07-03 First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children Yip, Christina Ignjatovic, Vera Attard, Chantal Monagle, Paul Linden, Matthew D. PLoS One Research Article Platelets are subcellular fragments which circulate in blood and have well established roles in thrombosis and haemostasis in adults. Upon activation, platelets undergo granule exocytosis and express P-Selectin on the cell membrane which binds a ligand on monocytes, leading to monocyte-platelet aggregation. Elevated circulating monocyte-platelet aggregates in adults are linked to atherothrombosis, but have not been investigated in children where thrombosis is less common. This study aimed to measure monocyte-platelet aggregate formation in children using whole blood flow cytometry. Monocyte-platelet aggregates as well as activation and granule exocytosis of platelets were measured in healthy adults (n = 15, median age 28 years) and healthy children (n = 28, median age 7 years). Monocyte-platelet aggregates in healthy children were elevated compared to healthy adults (37.8±4.4% vs 15.5±1.9% respectively, p<0.01). However, this was not accompanied by any difference in platelet activation (PAC-1 binding 6.8±1.5% vs 6.3±2.0% respectively, p = ns) or granule exocytosis (P-selectin expression 4.4±0.5% vs 3.1±0.5% respectively, p = ns). Despite comparable numbers of platelets bound per monocyte (GPIb MFI 117.3±13.7 vs 130.9±28.6 respectively, p = ns), surface P-selectin expression per platelet-bound monocyte was lower in children compared to adults. We therefore provide the first data of elevated monocyte-platelet aggregates in healthy children. Public Library of Science 2013-06-24 /pmc/articles/PMC3691142/ /pubmed/23826296 http://dx.doi.org/10.1371/journal.pone.0067416 Text en © 2013 Yip et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Yip, Christina Ignjatovic, Vera Attard, Chantal Monagle, Paul Linden, Matthew D. First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children |
title | First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children |
title_full | First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children |
title_fullStr | First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children |
title_full_unstemmed | First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children |
title_short | First Report of Elevated Monocyte-Platelet Aggregates in Healthy Children |
title_sort | first report of elevated monocyte-platelet aggregates in healthy children |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691142/ https://www.ncbi.nlm.nih.gov/pubmed/23826296 http://dx.doi.org/10.1371/journal.pone.0067416 |
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