Investigating Molecular Mechanisms of Embryonic Mammary Gland Development by Bead-Implantation in Embryonic Flank Explant Cultures – A Protocol

The involvement of molecular mechanisms in a particular process such as embryonic mammary gland development, can be revealed by modulation of one or several factors that purportedly act in that process. If those factors or their inhibitors are soluble, their function can be tested by loading them on...

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Detalles Bibliográficos
Autor principal: Veltmaat, Jacqueline M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691484/
https://www.ncbi.nlm.nih.gov/pubmed/23709170
http://dx.doi.org/10.1007/s10911-013-9297-1
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author Veltmaat, Jacqueline M.
author_facet Veltmaat, Jacqueline M.
author_sort Veltmaat, Jacqueline M.
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description The involvement of molecular mechanisms in a particular process such as embryonic mammary gland development, can be revealed by modulation of one or several factors that purportedly act in that process. If those factors or their inhibitors are soluble, their function can be tested by loading them onto small inert beads, which are then implanted in cultured explants of the tissue of interest, in this case embryonic flanks. We here describe a protocol for such experiments.
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spelling pubmed-36914842013-06-25 Investigating Molecular Mechanisms of Embryonic Mammary Gland Development by Bead-Implantation in Embryonic Flank Explant Cultures – A Protocol Veltmaat, Jacqueline M. J Mammary Gland Biol Neoplasia Article The involvement of molecular mechanisms in a particular process such as embryonic mammary gland development, can be revealed by modulation of one or several factors that purportedly act in that process. If those factors or their inhibitors are soluble, their function can be tested by loading them onto small inert beads, which are then implanted in cultured explants of the tissue of interest, in this case embryonic flanks. We here describe a protocol for such experiments. Springer US 2013-05-26 2013 /pmc/articles/PMC3691484/ /pubmed/23709170 http://dx.doi.org/10.1007/s10911-013-9297-1 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Veltmaat, Jacqueline M.
Investigating Molecular Mechanisms of Embryonic Mammary Gland Development by Bead-Implantation in Embryonic Flank Explant Cultures – A Protocol
title Investigating Molecular Mechanisms of Embryonic Mammary Gland Development by Bead-Implantation in Embryonic Flank Explant Cultures – A Protocol
title_full Investigating Molecular Mechanisms of Embryonic Mammary Gland Development by Bead-Implantation in Embryonic Flank Explant Cultures – A Protocol
title_fullStr Investigating Molecular Mechanisms of Embryonic Mammary Gland Development by Bead-Implantation in Embryonic Flank Explant Cultures – A Protocol
title_full_unstemmed Investigating Molecular Mechanisms of Embryonic Mammary Gland Development by Bead-Implantation in Embryonic Flank Explant Cultures – A Protocol
title_short Investigating Molecular Mechanisms of Embryonic Mammary Gland Development by Bead-Implantation in Embryonic Flank Explant Cultures – A Protocol
title_sort investigating molecular mechanisms of embryonic mammary gland development by bead-implantation in embryonic flank explant cultures – a protocol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691484/
https://www.ncbi.nlm.nih.gov/pubmed/23709170
http://dx.doi.org/10.1007/s10911-013-9297-1
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