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Effects of increase in fish oil intake on intestinal eicosanoids and inflammation in a mouse model of colitis

BACKGROUND: Inflammatory bowel diseases (IBD) are chronic intestinal inflammatory diseases affecting about 1% of western populations. New eating behaviors might contribute to the global emergence of IBD. Although the immunoregulatory effects of omega-3 fatty acids have been well characterized in vit...

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Autores principales: Bosco, Nabil, Brahmbhatt, Viral, Oliveira, Manuel, Martin, Francois-Pierre, Lichti, Pia, Raymond, Frederic, Mansourian, Robert, Metairon, Sylviane, Pace-Asciak, Cecil, Bastic Schmid, Viktoria, Rezzi, Serge, Haller, Dirk, Benyacoub, Jalil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691874/
https://www.ncbi.nlm.nih.gov/pubmed/23725086
http://dx.doi.org/10.1186/1476-511X-12-81
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author Bosco, Nabil
Brahmbhatt, Viral
Oliveira, Manuel
Martin, Francois-Pierre
Lichti, Pia
Raymond, Frederic
Mansourian, Robert
Metairon, Sylviane
Pace-Asciak, Cecil
Bastic Schmid, Viktoria
Rezzi, Serge
Haller, Dirk
Benyacoub, Jalil
author_facet Bosco, Nabil
Brahmbhatt, Viral
Oliveira, Manuel
Martin, Francois-Pierre
Lichti, Pia
Raymond, Frederic
Mansourian, Robert
Metairon, Sylviane
Pace-Asciak, Cecil
Bastic Schmid, Viktoria
Rezzi, Serge
Haller, Dirk
Benyacoub, Jalil
author_sort Bosco, Nabil
collection PubMed
description BACKGROUND: Inflammatory bowel diseases (IBD) are chronic intestinal inflammatory diseases affecting about 1% of western populations. New eating behaviors might contribute to the global emergence of IBD. Although the immunoregulatory effects of omega-3 fatty acids have been well characterized in vitro, their role in IBD is controversial. METHODS: The aim of this study was to assess the impact of increased fish oil intake on colonic gene expression, eicosanoid metabolism and development of colitis in a mouse model of IBD. Rag-2 deficient mice were fed fish oil (FO) enriched in omega-3 fatty acids i.e. EPA and DHA or control diet for 4 weeks before colitis induction by adoptive transfer of naïve T cells and maintained in the same diet for 4 additional weeks. Onset of colitis was monitored by colonoscopy and further confirmed by immunological examinations. Whole genome expression profiling was made and eicosanoids were measured by HPLC-MS/MS in colonic samples. RESULTS: A significant reduction of colonic proinflammatory eicosanoids in FO fed mice compared to control was observed. However, neither alteration of colonic gene expression signature nor reduction in IBD scores was observed under FO diet. CONCLUSION: Thus, increased intake of dietary FO did not prevent experimental colitis.
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spelling pubmed-36918742013-06-26 Effects of increase in fish oil intake on intestinal eicosanoids and inflammation in a mouse model of colitis Bosco, Nabil Brahmbhatt, Viral Oliveira, Manuel Martin, Francois-Pierre Lichti, Pia Raymond, Frederic Mansourian, Robert Metairon, Sylviane Pace-Asciak, Cecil Bastic Schmid, Viktoria Rezzi, Serge Haller, Dirk Benyacoub, Jalil Lipids Health Dis Research BACKGROUND: Inflammatory bowel diseases (IBD) are chronic intestinal inflammatory diseases affecting about 1% of western populations. New eating behaviors might contribute to the global emergence of IBD. Although the immunoregulatory effects of omega-3 fatty acids have been well characterized in vitro, their role in IBD is controversial. METHODS: The aim of this study was to assess the impact of increased fish oil intake on colonic gene expression, eicosanoid metabolism and development of colitis in a mouse model of IBD. Rag-2 deficient mice were fed fish oil (FO) enriched in omega-3 fatty acids i.e. EPA and DHA or control diet for 4 weeks before colitis induction by adoptive transfer of naïve T cells and maintained in the same diet for 4 additional weeks. Onset of colitis was monitored by colonoscopy and further confirmed by immunological examinations. Whole genome expression profiling was made and eicosanoids were measured by HPLC-MS/MS in colonic samples. RESULTS: A significant reduction of colonic proinflammatory eicosanoids in FO fed mice compared to control was observed. However, neither alteration of colonic gene expression signature nor reduction in IBD scores was observed under FO diet. CONCLUSION: Thus, increased intake of dietary FO did not prevent experimental colitis. BioMed Central 2013-05-31 /pmc/articles/PMC3691874/ /pubmed/23725086 http://dx.doi.org/10.1186/1476-511X-12-81 Text en Copyright © 2013 Bosco et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Bosco, Nabil
Brahmbhatt, Viral
Oliveira, Manuel
Martin, Francois-Pierre
Lichti, Pia
Raymond, Frederic
Mansourian, Robert
Metairon, Sylviane
Pace-Asciak, Cecil
Bastic Schmid, Viktoria
Rezzi, Serge
Haller, Dirk
Benyacoub, Jalil
Effects of increase in fish oil intake on intestinal eicosanoids and inflammation in a mouse model of colitis
title Effects of increase in fish oil intake on intestinal eicosanoids and inflammation in a mouse model of colitis
title_full Effects of increase in fish oil intake on intestinal eicosanoids and inflammation in a mouse model of colitis
title_fullStr Effects of increase in fish oil intake on intestinal eicosanoids and inflammation in a mouse model of colitis
title_full_unstemmed Effects of increase in fish oil intake on intestinal eicosanoids and inflammation in a mouse model of colitis
title_short Effects of increase in fish oil intake on intestinal eicosanoids and inflammation in a mouse model of colitis
title_sort effects of increase in fish oil intake on intestinal eicosanoids and inflammation in a mouse model of colitis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691874/
https://www.ncbi.nlm.nih.gov/pubmed/23725086
http://dx.doi.org/10.1186/1476-511X-12-81
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