Knockdown of the Placental Growth Factor Gene Inhibits Laser Induced Choroidal Neovascularization in a Murine Model

PURPOSE: To evaluate the effect of placental growth factor (PlGF) gene knockdown in a murine model of laser-induced choroidal neovascularization. METHODS: Choroidal neovascularization was induced in the left eyes of 11 mice by infrared laser. Small interfering RNA (siRNA, 20 picomoles/10 μl) corresp...

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Detalles Bibliográficos
Autores principales: Nourinia, Ramin, Soheili, Zahra-Soheila, Ahmadieh, Hamid, Akrami, Hassan, Rezaei Kanavi, Mozhgan, Samiei, Shahram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ophthalmic Research Center 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691981/
https://www.ncbi.nlm.nih.gov/pubmed/23825706
Descripción
Sumario:PURPOSE: To evaluate the effect of placental growth factor (PlGF) gene knockdown in a murine model of laser-induced choroidal neovascularization. METHODS: Choroidal neovascularization was induced in the left eyes of 11 mice by infrared laser. Small interfering RNA (siRNA, 20 picomoles/10 μl) corresponding to PlGF mRNA was administered intravitreally by Hamilton syringe in all subjects. One month later, fluorescein angiography and histolologic examination were performed. RESULTS: No leakage was apparent in the 11 eyes treated with siRNA cognate to PlGF. The results of histological evaluation were consistent with angiographic findings showing absence of choroidal neovascularization. CONCLUSION: Knockdown of the PlGF gene can inhibit the growth of laser-induced choroidal neovascularization in mice.

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