Knockdown of the Placental Growth Factor Gene Inhibits Laser Induced Choroidal Neovascularization in a Murine Model

PURPOSE: To evaluate the effect of placental growth factor (PlGF) gene knockdown in a murine model of laser-induced choroidal neovascularization. METHODS: Choroidal neovascularization was induced in the left eyes of 11 mice by infrared laser. Small interfering RNA (siRNA, 20 picomoles/10 μl) corresp...

Descripción completa

Detalles Bibliográficos
Autores principales: Nourinia, Ramin, Soheili, Zahra-Soheila, Ahmadieh, Hamid, Akrami, Hassan, Rezaei Kanavi, Mozhgan, Samiei, Shahram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ophthalmic Research Center 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691981/
https://www.ncbi.nlm.nih.gov/pubmed/23825706
_version_ 1782274553019367424
author Nourinia, Ramin
Soheili, Zahra-Soheila
Ahmadieh, Hamid
Akrami, Hassan
Rezaei Kanavi, Mozhgan
Samiei, Shahram
author_facet Nourinia, Ramin
Soheili, Zahra-Soheila
Ahmadieh, Hamid
Akrami, Hassan
Rezaei Kanavi, Mozhgan
Samiei, Shahram
author_sort Nourinia, Ramin
collection PubMed
description PURPOSE: To evaluate the effect of placental growth factor (PlGF) gene knockdown in a murine model of laser-induced choroidal neovascularization. METHODS: Choroidal neovascularization was induced in the left eyes of 11 mice by infrared laser. Small interfering RNA (siRNA, 20 picomoles/10 μl) corresponding to PlGF mRNA was administered intravitreally by Hamilton syringe in all subjects. One month later, fluorescein angiography and histolologic examination were performed. RESULTS: No leakage was apparent in the 11 eyes treated with siRNA cognate to PlGF. The results of histological evaluation were consistent with angiographic findings showing absence of choroidal neovascularization. CONCLUSION: Knockdown of the PlGF gene can inhibit the growth of laser-induced choroidal neovascularization in mice.
format Online
Article
Text
id pubmed-3691981
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Ophthalmic Research Center
record_format MEDLINE/PubMed
spelling pubmed-36919812013-07-02 Knockdown of the Placental Growth Factor Gene Inhibits Laser Induced Choroidal Neovascularization in a Murine Model Nourinia, Ramin Soheili, Zahra-Soheila Ahmadieh, Hamid Akrami, Hassan Rezaei Kanavi, Mozhgan Samiei, Shahram J Ophthalmic Vis Res Original Article PURPOSE: To evaluate the effect of placental growth factor (PlGF) gene knockdown in a murine model of laser-induced choroidal neovascularization. METHODS: Choroidal neovascularization was induced in the left eyes of 11 mice by infrared laser. Small interfering RNA (siRNA, 20 picomoles/10 μl) corresponding to PlGF mRNA was administered intravitreally by Hamilton syringe in all subjects. One month later, fluorescein angiography and histolologic examination were performed. RESULTS: No leakage was apparent in the 11 eyes treated with siRNA cognate to PlGF. The results of histological evaluation were consistent with angiographic findings showing absence of choroidal neovascularization. CONCLUSION: Knockdown of the PlGF gene can inhibit the growth of laser-induced choroidal neovascularization in mice. Ophthalmic Research Center 2013-01 /pmc/articles/PMC3691981/ /pubmed/23825706 Text en © 2013 Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Nourinia, Ramin
Soheili, Zahra-Soheila
Ahmadieh, Hamid
Akrami, Hassan
Rezaei Kanavi, Mozhgan
Samiei, Shahram
Knockdown of the Placental Growth Factor Gene Inhibits Laser Induced Choroidal Neovascularization in a Murine Model
title Knockdown of the Placental Growth Factor Gene Inhibits Laser Induced Choroidal Neovascularization in a Murine Model
title_full Knockdown of the Placental Growth Factor Gene Inhibits Laser Induced Choroidal Neovascularization in a Murine Model
title_fullStr Knockdown of the Placental Growth Factor Gene Inhibits Laser Induced Choroidal Neovascularization in a Murine Model
title_full_unstemmed Knockdown of the Placental Growth Factor Gene Inhibits Laser Induced Choroidal Neovascularization in a Murine Model
title_short Knockdown of the Placental Growth Factor Gene Inhibits Laser Induced Choroidal Neovascularization in a Murine Model
title_sort knockdown of the placental growth factor gene inhibits laser induced choroidal neovascularization in a murine model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691981/
https://www.ncbi.nlm.nih.gov/pubmed/23825706
work_keys_str_mv AT nouriniaramin knockdownoftheplacentalgrowthfactorgeneinhibitslaserinducedchoroidalneovascularizationinamurinemodel
AT soheilizahrasoheila knockdownoftheplacentalgrowthfactorgeneinhibitslaserinducedchoroidalneovascularizationinamurinemodel
AT ahmadiehhamid knockdownoftheplacentalgrowthfactorgeneinhibitslaserinducedchoroidalneovascularizationinamurinemodel
AT akramihassan knockdownoftheplacentalgrowthfactorgeneinhibitslaserinducedchoroidalneovascularizationinamurinemodel
AT rezaeikanavimozhgan knockdownoftheplacentalgrowthfactorgeneinhibitslaserinducedchoroidalneovascularizationinamurinemodel
AT samieishahram knockdownoftheplacentalgrowthfactorgeneinhibitslaserinducedchoroidalneovascularizationinamurinemodel

Ejemplares similares