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Titanium nanotubes activate genes related to bone formation in vitro
BACKGROUND: Titanium is used worldwide to make osseointegrable devices, thanks to its favorable characteristics as mechanical proprieties and biocompatibility, demonstrated by in vivo studies with animal models and clinical trials over a forty-year period. However, the exact genetic effect of the ti...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692167/ https://www.ncbi.nlm.nih.gov/pubmed/23814577 http://dx.doi.org/10.4103/1735-3327.109736 |
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author | Pozio, Alfonso Palmieri, Annalisa Girardi, Ambra Cura, Francesca Carinci, Francesco |
author_facet | Pozio, Alfonso Palmieri, Annalisa Girardi, Ambra Cura, Francesca Carinci, Francesco |
author_sort | Pozio, Alfonso |
collection | PubMed |
description | BACKGROUND: Titanium is used worldwide to make osseointegrable devices, thanks to its favorable characteristics as mechanical proprieties and biocompatibility, demonstrated by in vivo studies with animal models and clinical trials over a forty-year period. However, the exact genetic effect of the titanium layer on cells is still not well characterized. MATERIALS AND METHODS: To investigate how titanium nanotubes stimulate osteoblasts differentiation and proliferation, some osteoblast genes (SP7, RUNX2, COL3A1, COL1A1, ALPL, SPP1 and FOSL1) were analyzed by quantitative Real Time RT- PCR. RESULTS: After 15 days, osteoblasts cultivated on titanium naotube showed the up-regulation of bone related genes SP7, ENG, FOSL1 and SPP1 and the down-regulation of RUNX2, COL3A1, COL1A1, and ALPL. After 30 days of treatment, the bone related genes SP7, ENG, FOSL1 and RUNX2 were up-regulated while COL3A1, COL1A1, ALPL and SPP1 were down-regulated. CONCLUSIONS: Our results, demonstrates that titanium nanotubes can lead to osteoblast differentiation and extracellular matrix deposition and mineralization in dental pulp stem cells by the activation of osteoblast related genes SPP1, FOSL1 and RUNX2. |
format | Online Article Text |
id | pubmed-3692167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-36921672013-06-28 Titanium nanotubes activate genes related to bone formation in vitro Pozio, Alfonso Palmieri, Annalisa Girardi, Ambra Cura, Francesca Carinci, Francesco Dent Res J (Isfahan) Original Article BACKGROUND: Titanium is used worldwide to make osseointegrable devices, thanks to its favorable characteristics as mechanical proprieties and biocompatibility, demonstrated by in vivo studies with animal models and clinical trials over a forty-year period. However, the exact genetic effect of the titanium layer on cells is still not well characterized. MATERIALS AND METHODS: To investigate how titanium nanotubes stimulate osteoblasts differentiation and proliferation, some osteoblast genes (SP7, RUNX2, COL3A1, COL1A1, ALPL, SPP1 and FOSL1) were analyzed by quantitative Real Time RT- PCR. RESULTS: After 15 days, osteoblasts cultivated on titanium naotube showed the up-regulation of bone related genes SP7, ENG, FOSL1 and SPP1 and the down-regulation of RUNX2, COL3A1, COL1A1, and ALPL. After 30 days of treatment, the bone related genes SP7, ENG, FOSL1 and RUNX2 were up-regulated while COL3A1, COL1A1, ALPL and SPP1 were down-regulated. CONCLUSIONS: Our results, demonstrates that titanium nanotubes can lead to osteoblast differentiation and extracellular matrix deposition and mineralization in dental pulp stem cells by the activation of osteoblast related genes SPP1, FOSL1 and RUNX2. Medknow Publications & Media Pvt Ltd 2012-12 /pmc/articles/PMC3692167/ /pubmed/23814577 http://dx.doi.org/10.4103/1735-3327.109736 Text en Copyright: © Dental Research Journal http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Pozio, Alfonso Palmieri, Annalisa Girardi, Ambra Cura, Francesca Carinci, Francesco Titanium nanotubes activate genes related to bone formation in vitro |
title | Titanium nanotubes activate genes related to bone formation in vitro |
title_full | Titanium nanotubes activate genes related to bone formation in vitro |
title_fullStr | Titanium nanotubes activate genes related to bone formation in vitro |
title_full_unstemmed | Titanium nanotubes activate genes related to bone formation in vitro |
title_short | Titanium nanotubes activate genes related to bone formation in vitro |
title_sort | titanium nanotubes activate genes related to bone formation in vitro |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692167/ https://www.ncbi.nlm.nih.gov/pubmed/23814577 http://dx.doi.org/10.4103/1735-3327.109736 |
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