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Titanium nanotubes activate genes related to bone formation in vitro

BACKGROUND: Titanium is used worldwide to make osseointegrable devices, thanks to its favorable characteristics as mechanical proprieties and biocompatibility, demonstrated by in vivo studies with animal models and clinical trials over a forty-year period. However, the exact genetic effect of the ti...

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Autores principales: Pozio, Alfonso, Palmieri, Annalisa, Girardi, Ambra, Cura, Francesca, Carinci, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692167/
https://www.ncbi.nlm.nih.gov/pubmed/23814577
http://dx.doi.org/10.4103/1735-3327.109736
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author Pozio, Alfonso
Palmieri, Annalisa
Girardi, Ambra
Cura, Francesca
Carinci, Francesco
author_facet Pozio, Alfonso
Palmieri, Annalisa
Girardi, Ambra
Cura, Francesca
Carinci, Francesco
author_sort Pozio, Alfonso
collection PubMed
description BACKGROUND: Titanium is used worldwide to make osseointegrable devices, thanks to its favorable characteristics as mechanical proprieties and biocompatibility, demonstrated by in vivo studies with animal models and clinical trials over a forty-year period. However, the exact genetic effect of the titanium layer on cells is still not well characterized. MATERIALS AND METHODS: To investigate how titanium nanotubes stimulate osteoblasts differentiation and proliferation, some osteoblast genes (SP7, RUNX2, COL3A1, COL1A1, ALPL, SPP1 and FOSL1) were analyzed by quantitative Real Time RT- PCR. RESULTS: After 15 days, osteoblasts cultivated on titanium naotube showed the up-regulation of bone related genes SP7, ENG, FOSL1 and SPP1 and the down-regulation of RUNX2, COL3A1, COL1A1, and ALPL. After 30 days of treatment, the bone related genes SP7, ENG, FOSL1 and RUNX2 were up-regulated while COL3A1, COL1A1, ALPL and SPP1 were down-regulated. CONCLUSIONS: Our results, demonstrates that titanium nanotubes can lead to osteoblast differentiation and extracellular matrix deposition and mineralization in dental pulp stem cells by the activation of osteoblast related genes SPP1, FOSL1 and RUNX2.
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spelling pubmed-36921672013-06-28 Titanium nanotubes activate genes related to bone formation in vitro Pozio, Alfonso Palmieri, Annalisa Girardi, Ambra Cura, Francesca Carinci, Francesco Dent Res J (Isfahan) Original Article BACKGROUND: Titanium is used worldwide to make osseointegrable devices, thanks to its favorable characteristics as mechanical proprieties and biocompatibility, demonstrated by in vivo studies with animal models and clinical trials over a forty-year period. However, the exact genetic effect of the titanium layer on cells is still not well characterized. MATERIALS AND METHODS: To investigate how titanium nanotubes stimulate osteoblasts differentiation and proliferation, some osteoblast genes (SP7, RUNX2, COL3A1, COL1A1, ALPL, SPP1 and FOSL1) were analyzed by quantitative Real Time RT- PCR. RESULTS: After 15 days, osteoblasts cultivated on titanium naotube showed the up-regulation of bone related genes SP7, ENG, FOSL1 and SPP1 and the down-regulation of RUNX2, COL3A1, COL1A1, and ALPL. After 30 days of treatment, the bone related genes SP7, ENG, FOSL1 and RUNX2 were up-regulated while COL3A1, COL1A1, ALPL and SPP1 were down-regulated. CONCLUSIONS: Our results, demonstrates that titanium nanotubes can lead to osteoblast differentiation and extracellular matrix deposition and mineralization in dental pulp stem cells by the activation of osteoblast related genes SPP1, FOSL1 and RUNX2. Medknow Publications & Media Pvt Ltd 2012-12 /pmc/articles/PMC3692167/ /pubmed/23814577 http://dx.doi.org/10.4103/1735-3327.109736 Text en Copyright: © Dental Research Journal http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Pozio, Alfonso
Palmieri, Annalisa
Girardi, Ambra
Cura, Francesca
Carinci, Francesco
Titanium nanotubes activate genes related to bone formation in vitro
title Titanium nanotubes activate genes related to bone formation in vitro
title_full Titanium nanotubes activate genes related to bone formation in vitro
title_fullStr Titanium nanotubes activate genes related to bone formation in vitro
title_full_unstemmed Titanium nanotubes activate genes related to bone formation in vitro
title_short Titanium nanotubes activate genes related to bone formation in vitro
title_sort titanium nanotubes activate genes related to bone formation in vitro
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692167/
https://www.ncbi.nlm.nih.gov/pubmed/23814577
http://dx.doi.org/10.4103/1735-3327.109736
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