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Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody
The small molecule, meso-tetra(α,α,α,α-o-phenylacetamidophenyl) porphyrin (Mr1147.0) was used as complete antigen to elicit MAb through the immunization and cell fusion techniques. The MAb 1F2 obtained was demonstrated to be very pure by MALDI/TOFMS. The subtype of MAb 1F2 is IgG2a, which has a rela...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692307/ |
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author | Wang, Fengyang Huang, Xueying Du, Li Li, Weiguo He, Hongxuan Qi, Chao |
author_facet | Wang, Fengyang Huang, Xueying Du, Li Li, Weiguo He, Hongxuan Qi, Chao |
author_sort | Wang, Fengyang |
collection | PubMed |
description | The small molecule, meso-tetra(α,α,α,α-o-phenylacetamidophenyl) porphyrin (Mr1147.0) was used as complete antigen to elicit MAb through the immunization and cell fusion techniques. The MAb 1F2 obtained was demonstrated to be very pure by MALDI/TOFMS. The subtype of MAb 1F2 is IgG2a, which has a relative molecular weight of 156,678.8 Da.No significant change in the intensity of absorption peaks in UV and CD spectra was observed over a pH range between 6 and 12. The high stability of the abzyme and the tight binding between Fe porphyrin and antibody were also demonstrated. V(max), K(m), κcat, κcat/K(m) for abzyme are 5.18 × 10(−8) Ms(−1), 1.50 × 10(−8) M, 0.518 s(−1), 3.45 × 10(7) M(−1)s(−1), respectively. The data obtained indicate that catalytic antibody has high catalytic activity. The chloroperoxidase activity of MAb 1F2-Fe porphyrin complex is stable from 10 °C to 60 °C. |
format | Online Article Text |
id | pubmed-3692307 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-36923072013-06-26 Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody Wang, Fengyang Huang, Xueying Du, Li Li, Weiguo He, Hongxuan Qi, Chao Int J Mol Sci Full Research Paper The small molecule, meso-tetra(α,α,α,α-o-phenylacetamidophenyl) porphyrin (Mr1147.0) was used as complete antigen to elicit MAb through the immunization and cell fusion techniques. The MAb 1F2 obtained was demonstrated to be very pure by MALDI/TOFMS. The subtype of MAb 1F2 is IgG2a, which has a relative molecular weight of 156,678.8 Da.No significant change in the intensity of absorption peaks in UV and CD spectra was observed over a pH range between 6 and 12. The high stability of the abzyme and the tight binding between Fe porphyrin and antibody were also demonstrated. V(max), K(m), κcat, κcat/K(m) for abzyme are 5.18 × 10(−8) Ms(−1), 1.50 × 10(−8) M, 0.518 s(−1), 3.45 × 10(7) M(−1)s(−1), respectively. The data obtained indicate that catalytic antibody has high catalytic activity. The chloroperoxidase activity of MAb 1F2-Fe porphyrin complex is stable from 10 °C to 60 °C. Molecular Diversity Preservation International (MDPI) 2007-05-29 /pmc/articles/PMC3692307/ Text en © 2007 by MDPI Reproduction is permitted for noncommercial purposes. |
spellingShingle | Full Research Paper Wang, Fengyang Huang, Xueying Du, Li Li, Weiguo He, Hongxuan Qi, Chao Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody |
title | Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody |
title_full | Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody |
title_fullStr | Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody |
title_full_unstemmed | Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody |
title_short | Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody |
title_sort | preparation and characterization of a chloroperoxidase-like catalytic antibody |
topic | Full Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692307/ |
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