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Ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells

PURPOSE: Oxidative stress plays a key role in the pathophysiology of glaucoma. This study was designed to assess ethyl pyruvate (EP) as a novel antioxidative agent in cultured human trabecular meshwork (hTM) cells. METHODS: Primary hTM cells were cultured on collagen matrices. Tolerance to EP was as...

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Autores principales: Famili, Amin, Ammar, David A., Kahook, Malik Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692399/
https://www.ncbi.nlm.nih.gov/pubmed/23805037
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author Famili, Amin
Ammar, David A.
Kahook, Malik Y.
author_facet Famili, Amin
Ammar, David A.
Kahook, Malik Y.
author_sort Famili, Amin
collection PubMed
description PURPOSE: Oxidative stress plays a key role in the pathophysiology of glaucoma. This study was designed to assess ethyl pyruvate (EP) as a novel antioxidative agent in cultured human trabecular meshwork (hTM) cells. METHODS: Primary hTM cells were cultured on collagen matrices. Tolerance to EP was assessed at various concentrations using fluorescent vital dyes (live/dead) and metabolic (1-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. After the candidate doses were identified, cells received either preincubation with EP before hydrogen peroxide stressing or pre- and coincubation with EP before and during stressing. Live/dead and metabolic activity assays were used to quantify oxidative damage. RESULTS: Cultured hTM cells were well tolerant of EP concentrations at or below 10 mM while higher doses showed significant levels of cytotoxicity. In the peroxide stress assays, samples that received pre- and cotreatment with all concentrations of EP showed significantly increased cell survival and maintenance of metabolic activity. However, samples that received only pretreatment did not show a significant increase in survival rates and lost nearly all metabolic activity after peroxide-induced stressing. CONCLUSIONS: This work suggests that EP is a potent antioxidant that is well tolerated by hTM cells; however, EP’s potential as a therapeutic agent for glaucoma is limited by its inability to enhance endogenous antioxidant capacity. A continuous drug delivery system may be needed to realize the full therapeutic potential of EP for treatment of glaucoma.
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spelling pubmed-36923992013-06-26 Ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells Famili, Amin Ammar, David A. Kahook, Malik Y. Mol Vis Research Article PURPOSE: Oxidative stress plays a key role in the pathophysiology of glaucoma. This study was designed to assess ethyl pyruvate (EP) as a novel antioxidative agent in cultured human trabecular meshwork (hTM) cells. METHODS: Primary hTM cells were cultured on collagen matrices. Tolerance to EP was assessed at various concentrations using fluorescent vital dyes (live/dead) and metabolic (1-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. After the candidate doses were identified, cells received either preincubation with EP before hydrogen peroxide stressing or pre- and coincubation with EP before and during stressing. Live/dead and metabolic activity assays were used to quantify oxidative damage. RESULTS: Cultured hTM cells were well tolerant of EP concentrations at or below 10 mM while higher doses showed significant levels of cytotoxicity. In the peroxide stress assays, samples that received pre- and cotreatment with all concentrations of EP showed significantly increased cell survival and maintenance of metabolic activity. However, samples that received only pretreatment did not show a significant increase in survival rates and lost nearly all metabolic activity after peroxide-induced stressing. CONCLUSIONS: This work suggests that EP is a potent antioxidant that is well tolerated by hTM cells; however, EP’s potential as a therapeutic agent for glaucoma is limited by its inability to enhance endogenous antioxidant capacity. A continuous drug delivery system may be needed to realize the full therapeutic potential of EP for treatment of glaucoma. Molecular Vision 2013-06-11 /pmc/articles/PMC3692399/ /pubmed/23805037 Text en Copyright © 2013 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Famili, Amin
Ammar, David A.
Kahook, Malik Y.
Ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells
title Ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells
title_full Ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells
title_fullStr Ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells
title_full_unstemmed Ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells
title_short Ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells
title_sort ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692399/
https://www.ncbi.nlm.nih.gov/pubmed/23805037
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