Cargando…
A Longitudinal Study Simultaneously Exploring the Carriage of APEC Virulence Associated Genes and the Molecular Epidemiology of Faecal and Systemic E. coli in Commercial Broiler Chickens
Colibacillosis is an economically important syndromic disease of poultry caused by extra-intestinal avian pathogenic Escherichia coli (APEC) but the pathotype remains poorly defined. Combinations of virulence-associated genes (VAGs) have aided APEC identification. The intestinal microbiota is a pote...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692481/ https://www.ncbi.nlm.nih.gov/pubmed/23825682 http://dx.doi.org/10.1371/journal.pone.0067749 |
_version_ | 1782274622957289472 |
---|---|
author | Kemmett, Kirsty Humphrey, Tom Rushton, Steven Close, Andrew Wigley, Paul Williams, Nicola J. |
author_facet | Kemmett, Kirsty Humphrey, Tom Rushton, Steven Close, Andrew Wigley, Paul Williams, Nicola J. |
author_sort | Kemmett, Kirsty |
collection | PubMed |
description | Colibacillosis is an economically important syndromic disease of poultry caused by extra-intestinal avian pathogenic Escherichia coli (APEC) but the pathotype remains poorly defined. Combinations of virulence-associated genes (VAGs) have aided APEC identification. The intestinal microbiota is a potential APEC reservoir. Broiler chickens are selectively bred for fast, uniform growth. Here we simultaneously investigate intestinal E. coli VAG carriage in apparently healthy birds and characterise systemic E. coli from diseased broiler chickens from the same flocks. Four flocks were sampled longitudinally from chick placement until slaughter. Phylogrouping, macro-restriction pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were performed on an isolate subset from one flock to investigate the population structure of faecal and systemic E. coli. Early in production, VAG carriage among chick intestinal E. coli populations was diverse (average Simpson's D value = 0.73); 24.05% of intestinal E. coli (n = 160) from 1 day old chicks were carrying ≥5 VAGs. Generalised Linear models demonstrated VAG prevalence in potential APEC populations declined with age; 1% of E. coli carrying ≥5 VAGs at slaughter and demonstrated high strain diversity. A variety of VAG profiles and high strain diversity were observed among systemic E. coli. Thirty three new MLST sequence types were identified among 50 isolates and a new sequence type representing 22.2% (ST-2999) of the systemic population was found, differing from the pre-defined pathogenic ST-117 at a single locus. For the first time, this study takes a longitudinal approach to unravelling the APEC paradigm. Our findings, supported by other studies, highlight the difficulty in defining the APEC pathotype. Here we report a high genetic diversity among systemic E. coli between and within diseased broilers, harbouring diverse VAG profiles rather than single and/or highly related pathogenic clones suggesting host susceptibility in broilers plays an important role in APEC pathogenesis. |
format | Online Article Text |
id | pubmed-3692481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36924812013-07-02 A Longitudinal Study Simultaneously Exploring the Carriage of APEC Virulence Associated Genes and the Molecular Epidemiology of Faecal and Systemic E. coli in Commercial Broiler Chickens Kemmett, Kirsty Humphrey, Tom Rushton, Steven Close, Andrew Wigley, Paul Williams, Nicola J. PLoS One Research Article Colibacillosis is an economically important syndromic disease of poultry caused by extra-intestinal avian pathogenic Escherichia coli (APEC) but the pathotype remains poorly defined. Combinations of virulence-associated genes (VAGs) have aided APEC identification. The intestinal microbiota is a potential APEC reservoir. Broiler chickens are selectively bred for fast, uniform growth. Here we simultaneously investigate intestinal E. coli VAG carriage in apparently healthy birds and characterise systemic E. coli from diseased broiler chickens from the same flocks. Four flocks were sampled longitudinally from chick placement until slaughter. Phylogrouping, macro-restriction pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were performed on an isolate subset from one flock to investigate the population structure of faecal and systemic E. coli. Early in production, VAG carriage among chick intestinal E. coli populations was diverse (average Simpson's D value = 0.73); 24.05% of intestinal E. coli (n = 160) from 1 day old chicks were carrying ≥5 VAGs. Generalised Linear models demonstrated VAG prevalence in potential APEC populations declined with age; 1% of E. coli carrying ≥5 VAGs at slaughter and demonstrated high strain diversity. A variety of VAG profiles and high strain diversity were observed among systemic E. coli. Thirty three new MLST sequence types were identified among 50 isolates and a new sequence type representing 22.2% (ST-2999) of the systemic population was found, differing from the pre-defined pathogenic ST-117 at a single locus. For the first time, this study takes a longitudinal approach to unravelling the APEC paradigm. Our findings, supported by other studies, highlight the difficulty in defining the APEC pathotype. Here we report a high genetic diversity among systemic E. coli between and within diseased broilers, harbouring diverse VAG profiles rather than single and/or highly related pathogenic clones suggesting host susceptibility in broilers plays an important role in APEC pathogenesis. Public Library of Science 2013-06-25 /pmc/articles/PMC3692481/ /pubmed/23825682 http://dx.doi.org/10.1371/journal.pone.0067749 Text en © 2013 Kemmett et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kemmett, Kirsty Humphrey, Tom Rushton, Steven Close, Andrew Wigley, Paul Williams, Nicola J. A Longitudinal Study Simultaneously Exploring the Carriage of APEC Virulence Associated Genes and the Molecular Epidemiology of Faecal and Systemic E. coli in Commercial Broiler Chickens |
title | A Longitudinal Study Simultaneously Exploring the Carriage of APEC Virulence Associated Genes and the Molecular Epidemiology of Faecal and Systemic E. coli in Commercial Broiler Chickens |
title_full | A Longitudinal Study Simultaneously Exploring the Carriage of APEC Virulence Associated Genes and the Molecular Epidemiology of Faecal and Systemic E. coli in Commercial Broiler Chickens |
title_fullStr | A Longitudinal Study Simultaneously Exploring the Carriage of APEC Virulence Associated Genes and the Molecular Epidemiology of Faecal and Systemic E. coli in Commercial Broiler Chickens |
title_full_unstemmed | A Longitudinal Study Simultaneously Exploring the Carriage of APEC Virulence Associated Genes and the Molecular Epidemiology of Faecal and Systemic E. coli in Commercial Broiler Chickens |
title_short | A Longitudinal Study Simultaneously Exploring the Carriage of APEC Virulence Associated Genes and the Molecular Epidemiology of Faecal and Systemic E. coli in Commercial Broiler Chickens |
title_sort | longitudinal study simultaneously exploring the carriage of apec virulence associated genes and the molecular epidemiology of faecal and systemic e. coli in commercial broiler chickens |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692481/ https://www.ncbi.nlm.nih.gov/pubmed/23825682 http://dx.doi.org/10.1371/journal.pone.0067749 |
work_keys_str_mv | AT kemmettkirsty alongitudinalstudysimultaneouslyexploringthecarriageofapecvirulenceassociatedgenesandthemolecularepidemiologyoffaecalandsystemicecoliincommercialbroilerchickens AT humphreytom alongitudinalstudysimultaneouslyexploringthecarriageofapecvirulenceassociatedgenesandthemolecularepidemiologyoffaecalandsystemicecoliincommercialbroilerchickens AT rushtonsteven alongitudinalstudysimultaneouslyexploringthecarriageofapecvirulenceassociatedgenesandthemolecularepidemiologyoffaecalandsystemicecoliincommercialbroilerchickens AT closeandrew alongitudinalstudysimultaneouslyexploringthecarriageofapecvirulenceassociatedgenesandthemolecularepidemiologyoffaecalandsystemicecoliincommercialbroilerchickens AT wigleypaul alongitudinalstudysimultaneouslyexploringthecarriageofapecvirulenceassociatedgenesandthemolecularepidemiologyoffaecalandsystemicecoliincommercialbroilerchickens AT williamsnicolaj alongitudinalstudysimultaneouslyexploringthecarriageofapecvirulenceassociatedgenesandthemolecularepidemiologyoffaecalandsystemicecoliincommercialbroilerchickens AT kemmettkirsty longitudinalstudysimultaneouslyexploringthecarriageofapecvirulenceassociatedgenesandthemolecularepidemiologyoffaecalandsystemicecoliincommercialbroilerchickens AT humphreytom longitudinalstudysimultaneouslyexploringthecarriageofapecvirulenceassociatedgenesandthemolecularepidemiologyoffaecalandsystemicecoliincommercialbroilerchickens AT rushtonsteven longitudinalstudysimultaneouslyexploringthecarriageofapecvirulenceassociatedgenesandthemolecularepidemiologyoffaecalandsystemicecoliincommercialbroilerchickens AT closeandrew longitudinalstudysimultaneouslyexploringthecarriageofapecvirulenceassociatedgenesandthemolecularepidemiologyoffaecalandsystemicecoliincommercialbroilerchickens AT wigleypaul longitudinalstudysimultaneouslyexploringthecarriageofapecvirulenceassociatedgenesandthemolecularepidemiologyoffaecalandsystemicecoliincommercialbroilerchickens AT williamsnicolaj longitudinalstudysimultaneouslyexploringthecarriageofapecvirulenceassociatedgenesandthemolecularepidemiologyoffaecalandsystemicecoliincommercialbroilerchickens |