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Epidemiology and Genetic Diversity of Rotavirus Strains in Children with Acute Gastroenteritis in Lahore, Pakistan

Pakistan harbors high disease burden of gastro-enteric infections with majority of these caused by rotavirus. Unfortunately, lack of proper surveillance programs and laboratory facilities have resulted in scarcity of available data on rotavirus associated disease burden and epidemiological informati...

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Autores principales: Alam, Muhammad Masroor, Khurshid, Adnan, Shaukat, Shahzad, Suleman, Rana Muhammad, Sharif, Salmaan, Angez, Mehar, Malik, Salman Akbar, Ahmed, Tahir Masood, Aamir, Uzma Bashir, Naeem, Muhammad, Zaidi, Syed Sohail Zahoor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692488/
https://www.ncbi.nlm.nih.gov/pubmed/23825693
http://dx.doi.org/10.1371/journal.pone.0067998
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author Alam, Muhammad Masroor
Khurshid, Adnan
Shaukat, Shahzad
Suleman, Rana Muhammad
Sharif, Salmaan
Angez, Mehar
Malik, Salman Akbar
Ahmed, Tahir Masood
Aamir, Uzma Bashir
Naeem, Muhammad
Zaidi, Syed Sohail Zahoor
author_facet Alam, Muhammad Masroor
Khurshid, Adnan
Shaukat, Shahzad
Suleman, Rana Muhammad
Sharif, Salmaan
Angez, Mehar
Malik, Salman Akbar
Ahmed, Tahir Masood
Aamir, Uzma Bashir
Naeem, Muhammad
Zaidi, Syed Sohail Zahoor
author_sort Alam, Muhammad Masroor
collection PubMed
description Pakistan harbors high disease burden of gastro-enteric infections with majority of these caused by rotavirus. Unfortunately, lack of proper surveillance programs and laboratory facilities have resulted in scarcity of available data on rotavirus associated disease burden and epidemiological information in the country. We investigated 1306 stool samples collected over two years (2008–2009) from hospitalized children under 5 years of age for the presence of rotavirus strains and its genotypic diversity in Lahore. The prevalence rate during 2008 and 2009 was found to be 34% (n = 447 out of 1306). No significant difference was found between different age groups positive for rotavirus (p>0.05). A subset of EIA positive samples was further screened for rotavirus RNA through RT-PCR and 44 (49.43%) samples, out of total 89 EIA positive samples, were found positive. G and P type prevalence was found as follows: G1P [4] = 3(6.81%); G1P [6] = 9(20.45%); G1P [8] = 1(2.27%); G2P [4] = 21(47.72%); G2P [8] = 1(2.27%); G9P [4] = 1(2.27%); G9P [6] = 1(2.27%) and G9P [8] = 7(15.90%). Phylogenetic analysis revealed that the VP7 and VP4 sequences clustered closely with the previously detected strains in the country as well as Belgian rotaviruses. Antigenic characterization was performed by analyzing major epitopes in the immunodominant VP7 and VP4 gene segments. Although the neutralization conferring motifs were found variable between the Pakistani strains and the two recommended vaccines strains (Rotarix™ and RotaTeq™), we validate the use of rotavirus vaccine in Pakistan based on the proven and recognized vaccine efficacy across the globe. Our findings constitute the first report on rotavirus’ genotype diversity, their phylogenetic relatedness and epidemiology during the pre-vaccination era in Lahore, Pakistan and support the immediate introduction of rotavirus vaccine in the routine immunization program of the country.
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spelling pubmed-36924882013-07-02 Epidemiology and Genetic Diversity of Rotavirus Strains in Children with Acute Gastroenteritis in Lahore, Pakistan Alam, Muhammad Masroor Khurshid, Adnan Shaukat, Shahzad Suleman, Rana Muhammad Sharif, Salmaan Angez, Mehar Malik, Salman Akbar Ahmed, Tahir Masood Aamir, Uzma Bashir Naeem, Muhammad Zaidi, Syed Sohail Zahoor PLoS One Research Article Pakistan harbors high disease burden of gastro-enteric infections with majority of these caused by rotavirus. Unfortunately, lack of proper surveillance programs and laboratory facilities have resulted in scarcity of available data on rotavirus associated disease burden and epidemiological information in the country. We investigated 1306 stool samples collected over two years (2008–2009) from hospitalized children under 5 years of age for the presence of rotavirus strains and its genotypic diversity in Lahore. The prevalence rate during 2008 and 2009 was found to be 34% (n = 447 out of 1306). No significant difference was found between different age groups positive for rotavirus (p>0.05). A subset of EIA positive samples was further screened for rotavirus RNA through RT-PCR and 44 (49.43%) samples, out of total 89 EIA positive samples, were found positive. G and P type prevalence was found as follows: G1P [4] = 3(6.81%); G1P [6] = 9(20.45%); G1P [8] = 1(2.27%); G2P [4] = 21(47.72%); G2P [8] = 1(2.27%); G9P [4] = 1(2.27%); G9P [6] = 1(2.27%) and G9P [8] = 7(15.90%). Phylogenetic analysis revealed that the VP7 and VP4 sequences clustered closely with the previously detected strains in the country as well as Belgian rotaviruses. Antigenic characterization was performed by analyzing major epitopes in the immunodominant VP7 and VP4 gene segments. Although the neutralization conferring motifs were found variable between the Pakistani strains and the two recommended vaccines strains (Rotarix™ and RotaTeq™), we validate the use of rotavirus vaccine in Pakistan based on the proven and recognized vaccine efficacy across the globe. Our findings constitute the first report on rotavirus’ genotype diversity, their phylogenetic relatedness and epidemiology during the pre-vaccination era in Lahore, Pakistan and support the immediate introduction of rotavirus vaccine in the routine immunization program of the country. Public Library of Science 2013-06-25 /pmc/articles/PMC3692488/ /pubmed/23825693 http://dx.doi.org/10.1371/journal.pone.0067998 Text en © 2013 Alam et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Alam, Muhammad Masroor
Khurshid, Adnan
Shaukat, Shahzad
Suleman, Rana Muhammad
Sharif, Salmaan
Angez, Mehar
Malik, Salman Akbar
Ahmed, Tahir Masood
Aamir, Uzma Bashir
Naeem, Muhammad
Zaidi, Syed Sohail Zahoor
Epidemiology and Genetic Diversity of Rotavirus Strains in Children with Acute Gastroenteritis in Lahore, Pakistan
title Epidemiology and Genetic Diversity of Rotavirus Strains in Children with Acute Gastroenteritis in Lahore, Pakistan
title_full Epidemiology and Genetic Diversity of Rotavirus Strains in Children with Acute Gastroenteritis in Lahore, Pakistan
title_fullStr Epidemiology and Genetic Diversity of Rotavirus Strains in Children with Acute Gastroenteritis in Lahore, Pakistan
title_full_unstemmed Epidemiology and Genetic Diversity of Rotavirus Strains in Children with Acute Gastroenteritis in Lahore, Pakistan
title_short Epidemiology and Genetic Diversity of Rotavirus Strains in Children with Acute Gastroenteritis in Lahore, Pakistan
title_sort epidemiology and genetic diversity of rotavirus strains in children with acute gastroenteritis in lahore, pakistan
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692488/
https://www.ncbi.nlm.nih.gov/pubmed/23825693
http://dx.doi.org/10.1371/journal.pone.0067998
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