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Optimisation of Wavelength Modulated Raman Spectroscopy: Towards High Throughput Cell Screening

In the field of biomedicine, Raman spectroscopy is a powerful technique to discriminate between normal and cancerous cells. However the strong background signal from the sample and the instrumentation affects the efficiency of this discrimination technique. Wavelength Modulated Raman spectroscopy (W...

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Detalles Bibliográficos
Autores principales: Praveen, Bavishna B., Mazilu, Michael, Marchington, Robert F., Herrington, C. Simon, Riches, Andrew, Dholakia, Kishan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692494/
https://www.ncbi.nlm.nih.gov/pubmed/23825643
http://dx.doi.org/10.1371/journal.pone.0067211
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author Praveen, Bavishna B.
Mazilu, Michael
Marchington, Robert F.
Herrington, C. Simon
Riches, Andrew
Dholakia, Kishan
author_facet Praveen, Bavishna B.
Mazilu, Michael
Marchington, Robert F.
Herrington, C. Simon
Riches, Andrew
Dholakia, Kishan
author_sort Praveen, Bavishna B.
collection PubMed
description In the field of biomedicine, Raman spectroscopy is a powerful technique to discriminate between normal and cancerous cells. However the strong background signal from the sample and the instrumentation affects the efficiency of this discrimination technique. Wavelength Modulated Raman spectroscopy (WMRS) may suppress the background from the Raman spectra. In this study we demonstrate a systematic approach for optimizing the various parameters of WMRS to achieve a reduction in the acquisition time for potential applications such as higher throughput cell screening. The Signal to Noise Ratio (SNR) of the Raman bands depends on the modulation amplitude, time constant and total acquisition time. It was observed that the sampling rate does not influence the signal to noise ratio of the Raman bands if three or more wavelengths are sampled. With these optimised WMRS parameters, we increased the throughput in the binary classification of normal human urothelial cells and bladder cancer cells by reducing the total acquisition time to 6 s which is significantly lower in comparison to previous acquisition times required for the discrimination between similar cell types.
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spelling pubmed-36924942013-07-02 Optimisation of Wavelength Modulated Raman Spectroscopy: Towards High Throughput Cell Screening Praveen, Bavishna B. Mazilu, Michael Marchington, Robert F. Herrington, C. Simon Riches, Andrew Dholakia, Kishan PLoS One Research Article In the field of biomedicine, Raman spectroscopy is a powerful technique to discriminate between normal and cancerous cells. However the strong background signal from the sample and the instrumentation affects the efficiency of this discrimination technique. Wavelength Modulated Raman spectroscopy (WMRS) may suppress the background from the Raman spectra. In this study we demonstrate a systematic approach for optimizing the various parameters of WMRS to achieve a reduction in the acquisition time for potential applications such as higher throughput cell screening. The Signal to Noise Ratio (SNR) of the Raman bands depends on the modulation amplitude, time constant and total acquisition time. It was observed that the sampling rate does not influence the signal to noise ratio of the Raman bands if three or more wavelengths are sampled. With these optimised WMRS parameters, we increased the throughput in the binary classification of normal human urothelial cells and bladder cancer cells by reducing the total acquisition time to 6 s which is significantly lower in comparison to previous acquisition times required for the discrimination between similar cell types. Public Library of Science 2013-06-25 /pmc/articles/PMC3692494/ /pubmed/23825643 http://dx.doi.org/10.1371/journal.pone.0067211 Text en © 2013 Praveen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Praveen, Bavishna B.
Mazilu, Michael
Marchington, Robert F.
Herrington, C. Simon
Riches, Andrew
Dholakia, Kishan
Optimisation of Wavelength Modulated Raman Spectroscopy: Towards High Throughput Cell Screening
title Optimisation of Wavelength Modulated Raman Spectroscopy: Towards High Throughput Cell Screening
title_full Optimisation of Wavelength Modulated Raman Spectroscopy: Towards High Throughput Cell Screening
title_fullStr Optimisation of Wavelength Modulated Raman Spectroscopy: Towards High Throughput Cell Screening
title_full_unstemmed Optimisation of Wavelength Modulated Raman Spectroscopy: Towards High Throughput Cell Screening
title_short Optimisation of Wavelength Modulated Raman Spectroscopy: Towards High Throughput Cell Screening
title_sort optimisation of wavelength modulated raman spectroscopy: towards high throughput cell screening
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692494/
https://www.ncbi.nlm.nih.gov/pubmed/23825643
http://dx.doi.org/10.1371/journal.pone.0067211
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