Cargando…

Urinary Total Protein as the Predictor of Albuminuria in Diabetic Patients

BACKGROUND: In order to detect nephropathy, measurement of total (24 hrs) urinary albumin or albumin/creatinin ratio in random urine samples is being recommended. But methods of albumin measurement are not available in all laboratories and also cost about 6 times more than that of urinary total prot...

Descripción completa

Detalles Bibliográficos
Autores principales: Hashemipour, Sima, Charkhchian, Maliheh, Javadi, Amir, Afaghi, Ahmad, Hajiaghamohamadi, Ali Akbar, Bastani, Ali, Hajmanoochehri, Fateme, Ziaee, Amir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693628/
https://www.ncbi.nlm.nih.gov/pubmed/23843814
http://dx.doi.org/10.5812/ijem.4236
_version_ 1782274738612076544
author Hashemipour, Sima
Charkhchian, Maliheh
Javadi, Amir
Afaghi, Ahmad
Hajiaghamohamadi, Ali Akbar
Bastani, Ali
Hajmanoochehri, Fateme
Ziaee, Amir
author_facet Hashemipour, Sima
Charkhchian, Maliheh
Javadi, Amir
Afaghi, Ahmad
Hajiaghamohamadi, Ali Akbar
Bastani, Ali
Hajmanoochehri, Fateme
Ziaee, Amir
author_sort Hashemipour, Sima
collection PubMed
description BACKGROUND: In order to detect nephropathy, measurement of total (24 hrs) urinary albumin or albumin/creatinin ratio in random urine samples is being recommended. But methods of albumin measurement are not available in all laboratories and also cost about 6 times more than that of urinary total protein measurement. OBJECTIVES: This Study was performed to determine appropriate cut off point in 24 hours urine total protein to diagnose micro- and macroalbuminuria in patients with diabetes mellitus. PATIENTS AND METHODS: In this study, 204 patients with diabetes mellitus type I and II were selected. In collected 24 hours urine from patients, protein and albumin were measured by using Pyrogallol and Immunoturbidimetry methods, respectively. RESULTS: Normoalbuminuri (albumin < 30 mg/24 hrs urine), microalbuminuri (albumin = 30-300 mg/24 hrs urine), and macroalbuminuri (albumin > 300 mg/24 hrs urine) were detected in 130, 51, and 23 patients, respectively. In 24 hrs urine collections, amounts of protein and albumin were compared to calculate cut off point of exerted protein for nephropathy diagnosis. cut off point of 73 mg/day for urinary total protein had appropriate sensitivity (94.5 %, CI = 91.4 % -97.6 %) and specificity (77.9 %, CI = 72.8 % -82.9 %) for microalbuminuria, while cut off point of 514 mg/day (sensitivity 95.7 %; specificity 98.9 %) was detected for diagnosis macroalbuminuria. Urine protein exertion of 150 mg/day that is currently considered as a normal value in most laboratory kits had a sensitivity of 73.1 % by which 30 % of microalbuminuric cases remained undiagnosed. CONCLUSIONS: Urinary total protein cut-off points of 73 mg/day and 514 mg/day were diagnostic for micro- and macroalbuminuria, respectively.
format Online
Article
Text
id pubmed-3693628
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Kowsar
record_format MEDLINE/PubMed
spelling pubmed-36936282013-07-10 Urinary Total Protein as the Predictor of Albuminuria in Diabetic Patients Hashemipour, Sima Charkhchian, Maliheh Javadi, Amir Afaghi, Ahmad Hajiaghamohamadi, Ali Akbar Bastani, Ali Hajmanoochehri, Fateme Ziaee, Amir Int J Endocrinol Metab Original Article BACKGROUND: In order to detect nephropathy, measurement of total (24 hrs) urinary albumin or albumin/creatinin ratio in random urine samples is being recommended. But methods of albumin measurement are not available in all laboratories and also cost about 6 times more than that of urinary total protein measurement. OBJECTIVES: This Study was performed to determine appropriate cut off point in 24 hours urine total protein to diagnose micro- and macroalbuminuria in patients with diabetes mellitus. PATIENTS AND METHODS: In this study, 204 patients with diabetes mellitus type I and II were selected. In collected 24 hours urine from patients, protein and albumin were measured by using Pyrogallol and Immunoturbidimetry methods, respectively. RESULTS: Normoalbuminuri (albumin < 30 mg/24 hrs urine), microalbuminuri (albumin = 30-300 mg/24 hrs urine), and macroalbuminuri (albumin > 300 mg/24 hrs urine) were detected in 130, 51, and 23 patients, respectively. In 24 hrs urine collections, amounts of protein and albumin were compared to calculate cut off point of exerted protein for nephropathy diagnosis. cut off point of 73 mg/day for urinary total protein had appropriate sensitivity (94.5 %, CI = 91.4 % -97.6 %) and specificity (77.9 %, CI = 72.8 % -82.9 %) for microalbuminuria, while cut off point of 514 mg/day (sensitivity 95.7 %; specificity 98.9 %) was detected for diagnosis macroalbuminuria. Urine protein exertion of 150 mg/day that is currently considered as a normal value in most laboratory kits had a sensitivity of 73.1 % by which 30 % of microalbuminuric cases remained undiagnosed. CONCLUSIONS: Urinary total protein cut-off points of 73 mg/day and 514 mg/day were diagnostic for micro- and macroalbuminuria, respectively. Kowsar 2012-06-30 2012 /pmc/articles/PMC3693628/ /pubmed/23843814 http://dx.doi.org/10.5812/ijem.4236 Text en Copyright © 2012, Research Institute For Endocrine Sciences and Iran Endocrine Society http://creativecommons.org/licenses/by/3/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hashemipour, Sima
Charkhchian, Maliheh
Javadi, Amir
Afaghi, Ahmad
Hajiaghamohamadi, Ali Akbar
Bastani, Ali
Hajmanoochehri, Fateme
Ziaee, Amir
Urinary Total Protein as the Predictor of Albuminuria in Diabetic Patients
title Urinary Total Protein as the Predictor of Albuminuria in Diabetic Patients
title_full Urinary Total Protein as the Predictor of Albuminuria in Diabetic Patients
title_fullStr Urinary Total Protein as the Predictor of Albuminuria in Diabetic Patients
title_full_unstemmed Urinary Total Protein as the Predictor of Albuminuria in Diabetic Patients
title_short Urinary Total Protein as the Predictor of Albuminuria in Diabetic Patients
title_sort urinary total protein as the predictor of albuminuria in diabetic patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693628/
https://www.ncbi.nlm.nih.gov/pubmed/23843814
http://dx.doi.org/10.5812/ijem.4236
work_keys_str_mv AT hashemipoursima urinarytotalproteinasthepredictorofalbuminuriaindiabeticpatients
AT charkhchianmaliheh urinarytotalproteinasthepredictorofalbuminuriaindiabeticpatients
AT javadiamir urinarytotalproteinasthepredictorofalbuminuriaindiabeticpatients
AT afaghiahmad urinarytotalproteinasthepredictorofalbuminuriaindiabeticpatients
AT hajiaghamohamadialiakbar urinarytotalproteinasthepredictorofalbuminuriaindiabeticpatients
AT bastaniali urinarytotalproteinasthepredictorofalbuminuriaindiabeticpatients
AT hajmanoochehrifateme urinarytotalproteinasthepredictorofalbuminuriaindiabeticpatients
AT ziaeeamir urinarytotalproteinasthepredictorofalbuminuriaindiabeticpatients