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Priming of Production in Maize of Volatile Organic Defence Compounds by the Natural Plant Activator cis-Jasmone

cis-Jasmone (CJ) is a natural plant product that activates defence against herbivores in model and crop plants. In this study, we investigated whether CJ could prime defence in maize, Zea mays, against the leafhopper, Cicadulina storeyi, responsible for the transmission of maize streak virus (MSV)....

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Autores principales: Oluwafemi, Sunday, Dewhirst, Sarah Y., Veyrat, Nathalie, Powers, Stephen, Bruce, Toby J. A., Caulfield, John C., Pickett, John A., Birkett, Michael A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694093/
https://www.ncbi.nlm.nih.gov/pubmed/23840295
http://dx.doi.org/10.1371/journal.pone.0062299
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author Oluwafemi, Sunday
Dewhirst, Sarah Y.
Veyrat, Nathalie
Powers, Stephen
Bruce, Toby J. A.
Caulfield, John C.
Pickett, John A.
Birkett, Michael A.
author_facet Oluwafemi, Sunday
Dewhirst, Sarah Y.
Veyrat, Nathalie
Powers, Stephen
Bruce, Toby J. A.
Caulfield, John C.
Pickett, John A.
Birkett, Michael A.
author_sort Oluwafemi, Sunday
collection PubMed
description cis-Jasmone (CJ) is a natural plant product that activates defence against herbivores in model and crop plants. In this study, we investigated whether CJ could prime defence in maize, Zea mays, against the leafhopper, Cicadulina storeyi, responsible for the transmission of maize streak virus (MSV). Priming occurs when a pre-treatment, in this case CJ, increases the potency and speed of a defence response upon subsequent attack on the plant. Here, we tested insect responses to plant volatile organic compounds (VOCs) using a Y-tube olfactometer bioassay. Our initial experiments showed that, in this system, there was no significant response of the herbivore to CJ itself and no difference in response to VOCs collected from unexposed plants compared to CJ exposed plants, both without insects. VOCs were then collected from C. storeyi-infested maize seedlings with and without CJ pre-treatment. The bioassay revealed a significant preference by this pest for VOCs from infested seedlings without the CJ pre-treatment. A timed series of VOC collections and bioassays showed that the effect was strongest in the first 22 h of insect infestation, i.e. before the insects had themselves induced a change in VOC emission. Chemical analysis showed that treatment of maize seedlings with CJ, followed by exposure to C. storeyi, led to a significant increase in emission of the defensive sesquiterpenes (E)-(1R,9S)-caryophyllene, (E)-α-bergamotene, (E)-β-farnesene and (E)-4,8-dimethyl-1,3,7-nonatriene, known to act as herbivore repellents. The chemical analysis explains the behavioural effects observed in the olfactometer, as the CJ treatment caused plants to emit a blend of VOCs comprising more of the repellent components in the first 22 h of insect infestation than control plants. The speed and potency of VOC emission was increased by the CJ pre-treatment. This is the first indication that CJ can prime plants for enhanced production of defensive VOCs antagonist towards herbivores.
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spelling pubmed-36940932013-07-09 Priming of Production in Maize of Volatile Organic Defence Compounds by the Natural Plant Activator cis-Jasmone Oluwafemi, Sunday Dewhirst, Sarah Y. Veyrat, Nathalie Powers, Stephen Bruce, Toby J. A. Caulfield, John C. Pickett, John A. Birkett, Michael A. PLoS One Research Article cis-Jasmone (CJ) is a natural plant product that activates defence against herbivores in model and crop plants. In this study, we investigated whether CJ could prime defence in maize, Zea mays, against the leafhopper, Cicadulina storeyi, responsible for the transmission of maize streak virus (MSV). Priming occurs when a pre-treatment, in this case CJ, increases the potency and speed of a defence response upon subsequent attack on the plant. Here, we tested insect responses to plant volatile organic compounds (VOCs) using a Y-tube olfactometer bioassay. Our initial experiments showed that, in this system, there was no significant response of the herbivore to CJ itself and no difference in response to VOCs collected from unexposed plants compared to CJ exposed plants, both without insects. VOCs were then collected from C. storeyi-infested maize seedlings with and without CJ pre-treatment. The bioassay revealed a significant preference by this pest for VOCs from infested seedlings without the CJ pre-treatment. A timed series of VOC collections and bioassays showed that the effect was strongest in the first 22 h of insect infestation, i.e. before the insects had themselves induced a change in VOC emission. Chemical analysis showed that treatment of maize seedlings with CJ, followed by exposure to C. storeyi, led to a significant increase in emission of the defensive sesquiterpenes (E)-(1R,9S)-caryophyllene, (E)-α-bergamotene, (E)-β-farnesene and (E)-4,8-dimethyl-1,3,7-nonatriene, known to act as herbivore repellents. The chemical analysis explains the behavioural effects observed in the olfactometer, as the CJ treatment caused plants to emit a blend of VOCs comprising more of the repellent components in the first 22 h of insect infestation than control plants. The speed and potency of VOC emission was increased by the CJ pre-treatment. This is the first indication that CJ can prime plants for enhanced production of defensive VOCs antagonist towards herbivores. Public Library of Science 2013-06-26 /pmc/articles/PMC3694093/ /pubmed/23840295 http://dx.doi.org/10.1371/journal.pone.0062299 Text en © 2013 Oluwafemi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Oluwafemi, Sunday
Dewhirst, Sarah Y.
Veyrat, Nathalie
Powers, Stephen
Bruce, Toby J. A.
Caulfield, John C.
Pickett, John A.
Birkett, Michael A.
Priming of Production in Maize of Volatile Organic Defence Compounds by the Natural Plant Activator cis-Jasmone
title Priming of Production in Maize of Volatile Organic Defence Compounds by the Natural Plant Activator cis-Jasmone
title_full Priming of Production in Maize of Volatile Organic Defence Compounds by the Natural Plant Activator cis-Jasmone
title_fullStr Priming of Production in Maize of Volatile Organic Defence Compounds by the Natural Plant Activator cis-Jasmone
title_full_unstemmed Priming of Production in Maize of Volatile Organic Defence Compounds by the Natural Plant Activator cis-Jasmone
title_short Priming of Production in Maize of Volatile Organic Defence Compounds by the Natural Plant Activator cis-Jasmone
title_sort priming of production in maize of volatile organic defence compounds by the natural plant activator cis-jasmone
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694093/
https://www.ncbi.nlm.nih.gov/pubmed/23840295
http://dx.doi.org/10.1371/journal.pone.0062299
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