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A simple tool to improve pluripotent stem cell differentiation

We develop a method to overcome previously documented restrictions on the differentiation propensities of pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cyc...

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Detalles Bibliográficos
Autores principales: Chetty, Sundari, Pagliuca, Felicia Walton, Honore, Christian, Kweudjeu, Anastasie, Rezania, Alireza, Melton, Douglas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694177/
https://www.ncbi.nlm.nih.gov/pubmed/23584186
http://dx.doi.org/10.1038/nmeth.2442
Descripción
Sumario:We develop a method to overcome previously documented restrictions on the differentiation propensities of pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle, and subsequently improves their competency for directed differentiation into multiple lineages in more than 25 stem cell lines. DMSO treatment also promotes terminal differentiation into functional derivatives.