A simple tool to improve pluripotent stem cell differentiation

We develop a method to overcome previously documented restrictions on the differentiation propensities of pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cyc...

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Detalles Bibliográficos
Autores principales: Chetty, Sundari, Pagliuca, Felicia Walton, Honore, Christian, Kweudjeu, Anastasie, Rezania, Alireza, Melton, Douglas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694177/
https://www.ncbi.nlm.nih.gov/pubmed/23584186
http://dx.doi.org/10.1038/nmeth.2442
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author Chetty, Sundari
Pagliuca, Felicia Walton
Honore, Christian
Kweudjeu, Anastasie
Rezania, Alireza
Melton, Douglas A.
author_facet Chetty, Sundari
Pagliuca, Felicia Walton
Honore, Christian
Kweudjeu, Anastasie
Rezania, Alireza
Melton, Douglas A.
author_sort Chetty, Sundari
collection PubMed
description We develop a method to overcome previously documented restrictions on the differentiation propensities of pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle, and subsequently improves their competency for directed differentiation into multiple lineages in more than 25 stem cell lines. DMSO treatment also promotes terminal differentiation into functional derivatives.
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spelling pubmed-36941772013-12-01 A simple tool to improve pluripotent stem cell differentiation Chetty, Sundari Pagliuca, Felicia Walton Honore, Christian Kweudjeu, Anastasie Rezania, Alireza Melton, Douglas A. Nat Methods Article We develop a method to overcome previously documented restrictions on the differentiation propensities of pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle, and subsequently improves their competency for directed differentiation into multiple lineages in more than 25 stem cell lines. DMSO treatment also promotes terminal differentiation into functional derivatives. 2013-04-14 2013-06 /pmc/articles/PMC3694177/ /pubmed/23584186 http://dx.doi.org/10.1038/nmeth.2442 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Chetty, Sundari
Pagliuca, Felicia Walton
Honore, Christian
Kweudjeu, Anastasie
Rezania, Alireza
Melton, Douglas A.
A simple tool to improve pluripotent stem cell differentiation
title A simple tool to improve pluripotent stem cell differentiation
title_full A simple tool to improve pluripotent stem cell differentiation
title_fullStr A simple tool to improve pluripotent stem cell differentiation
title_full_unstemmed A simple tool to improve pluripotent stem cell differentiation
title_short A simple tool to improve pluripotent stem cell differentiation
title_sort simple tool to improve pluripotent stem cell differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694177/
https://www.ncbi.nlm.nih.gov/pubmed/23584186
http://dx.doi.org/10.1038/nmeth.2442
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