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Environmental Dependence of Genetic Constraint

The epistatic interactions that underlie evolutionary constraint have mainly been studied for constant external conditions. However, environmental changes may modulate epistasis and hence affect genetic constraints. Here we investigate genetic constraints in the adaptive evolution of a novel regulat...

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Detalles Bibliográficos
Autores principales: de Vos, Marjon G. J., Poelwijk, Frank J., Battich, Nico, Ndika, Joseph D. T., Tans, Sander J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694820/
https://www.ncbi.nlm.nih.gov/pubmed/23825963
http://dx.doi.org/10.1371/journal.pgen.1003580
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author de Vos, Marjon G. J.
Poelwijk, Frank J.
Battich, Nico
Ndika, Joseph D. T.
Tans, Sander J.
author_facet de Vos, Marjon G. J.
Poelwijk, Frank J.
Battich, Nico
Ndika, Joseph D. T.
Tans, Sander J.
author_sort de Vos, Marjon G. J.
collection PubMed
description The epistatic interactions that underlie evolutionary constraint have mainly been studied for constant external conditions. However, environmental changes may modulate epistasis and hence affect genetic constraints. Here we investigate genetic constraints in the adaptive evolution of a novel regulatory function in variable environments, using the lac repressor, LacI, as a model system. We have systematically reconstructed mutational trajectories from wild type LacI to three different variants that each exhibit an inverse response to the inducing ligand IPTG, and analyzed the higher-order interactions between genetic and environmental changes. We find epistasis to depend strongly on the environment. As a result, mutational steps essential to inversion but inaccessible by positive selection in one environment, become accessible in another. We present a graphical method to analyze the observed complex higher-order interactions between multiple mutations and environmental change, and show how the interactions can be explained by a combination of mutational effects on allostery and thermodynamic stability. This dependency of genetic constraint on the environment should fundamentally affect evolutionary dynamics and affects the interpretation of phylogenetic data.
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spelling pubmed-36948202013-07-03 Environmental Dependence of Genetic Constraint de Vos, Marjon G. J. Poelwijk, Frank J. Battich, Nico Ndika, Joseph D. T. Tans, Sander J. PLoS Genet Research Article The epistatic interactions that underlie evolutionary constraint have mainly been studied for constant external conditions. However, environmental changes may modulate epistasis and hence affect genetic constraints. Here we investigate genetic constraints in the adaptive evolution of a novel regulatory function in variable environments, using the lac repressor, LacI, as a model system. We have systematically reconstructed mutational trajectories from wild type LacI to three different variants that each exhibit an inverse response to the inducing ligand IPTG, and analyzed the higher-order interactions between genetic and environmental changes. We find epistasis to depend strongly on the environment. As a result, mutational steps essential to inversion but inaccessible by positive selection in one environment, become accessible in another. We present a graphical method to analyze the observed complex higher-order interactions between multiple mutations and environmental change, and show how the interactions can be explained by a combination of mutational effects on allostery and thermodynamic stability. This dependency of genetic constraint on the environment should fundamentally affect evolutionary dynamics and affects the interpretation of phylogenetic data. Public Library of Science 2013-06-27 /pmc/articles/PMC3694820/ /pubmed/23825963 http://dx.doi.org/10.1371/journal.pgen.1003580 Text en © 2013 de Vos et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
de Vos, Marjon G. J.
Poelwijk, Frank J.
Battich, Nico
Ndika, Joseph D. T.
Tans, Sander J.
Environmental Dependence of Genetic Constraint
title Environmental Dependence of Genetic Constraint
title_full Environmental Dependence of Genetic Constraint
title_fullStr Environmental Dependence of Genetic Constraint
title_full_unstemmed Environmental Dependence of Genetic Constraint
title_short Environmental Dependence of Genetic Constraint
title_sort environmental dependence of genetic constraint
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694820/
https://www.ncbi.nlm.nih.gov/pubmed/23825963
http://dx.doi.org/10.1371/journal.pgen.1003580
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