Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and meta...

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Detalles Bibliográficos
Autores principales: Uguru, Gabriel C., Mondhe, Madhav, Goh, Shan, Hesketh, Andrew, Bibb, Mervyn J., Good, Liam, Stach, James E. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694883/
https://www.ncbi.nlm.nih.gov/pubmed/23826310
http://dx.doi.org/10.1371/journal.pone.0067509
Descripción
Sumario:We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.

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