Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and meta...

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Detalles Bibliográficos
Autores principales: Uguru, Gabriel C., Mondhe, Madhav, Goh, Shan, Hesketh, Andrew, Bibb, Mervyn J., Good, Liam, Stach, James E. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694883/
https://www.ncbi.nlm.nih.gov/pubmed/23826310
http://dx.doi.org/10.1371/journal.pone.0067509
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author Uguru, Gabriel C.
Mondhe, Madhav
Goh, Shan
Hesketh, Andrew
Bibb, Mervyn J.
Good, Liam
Stach, James E. M.
author_facet Uguru, Gabriel C.
Mondhe, Madhav
Goh, Shan
Hesketh, Andrew
Bibb, Mervyn J.
Good, Liam
Stach, James E. M.
author_sort Uguru, Gabriel C.
collection PubMed
description We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.
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spelling pubmed-36948832013-07-03 Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2) Uguru, Gabriel C. Mondhe, Madhav Goh, Shan Hesketh, Andrew Bibb, Mervyn J. Good, Liam Stach, James E. M. PLoS One Research Article We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces. Public Library of Science 2013-06-27 /pmc/articles/PMC3694883/ /pubmed/23826310 http://dx.doi.org/10.1371/journal.pone.0067509 Text en © 2013 Uguru et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Uguru, Gabriel C.
Mondhe, Madhav
Goh, Shan
Hesketh, Andrew
Bibb, Mervyn J.
Good, Liam
Stach, James E. M.
Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)
title Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)
title_full Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)
title_fullStr Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)
title_full_unstemmed Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)
title_short Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)
title_sort synthetic rna silencing of actinorhodin biosynthesis in streptomyces coelicolor a3(2)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694883/
https://www.ncbi.nlm.nih.gov/pubmed/23826310
http://dx.doi.org/10.1371/journal.pone.0067509
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