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Massive functional mapping of a 5′-UTR by saturation mutagenesis, phenotypic sorting and deep sequencing
We present here a method that enables functional screening of large number of mutations in a single experiment through the combination of random mutagenesis, phenotypic cell sorting and high-throughput sequencing. As a test case, we studied post-transcriptional gene regulation of the bacterial csgD...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695526/ https://www.ncbi.nlm.nih.gov/pubmed/23609548 http://dx.doi.org/10.1093/nar/gkt267 |