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VAMP4 is required to maintain the ribbon structure of the Golgi apparatus

The Golgi apparatus forms a twisted ribbon-like network in the juxtanuclear region of vertebrate cells. Vesicle-associated membrane protein 4 (VAMP4), a v-SNARE protein expressed exclusively in the vertebrate trans-Golgi network (TGN), plays a role in retrograde trafficking from the early endosome t...

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Autores principales: Shitara, Akiko, Shibui, Toru, Okayama, Miki, Arakawa, Toshiya, Mizoguchi, Itaru, Shakakura, Yasunori, Takuma, Taishin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695666/
https://www.ncbi.nlm.nih.gov/pubmed/23677696
http://dx.doi.org/10.1007/s11010-013-1652-4
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author Shitara, Akiko
Shibui, Toru
Okayama, Miki
Arakawa, Toshiya
Mizoguchi, Itaru
Shakakura, Yasunori
Takuma, Taishin
author_facet Shitara, Akiko
Shibui, Toru
Okayama, Miki
Arakawa, Toshiya
Mizoguchi, Itaru
Shakakura, Yasunori
Takuma, Taishin
author_sort Shitara, Akiko
collection PubMed
description The Golgi apparatus forms a twisted ribbon-like network in the juxtanuclear region of vertebrate cells. Vesicle-associated membrane protein 4 (VAMP4), a v-SNARE protein expressed exclusively in the vertebrate trans-Golgi network (TGN), plays a role in retrograde trafficking from the early endosome to the TGN, although its precise function within the Golgi apparatus remains unclear. To determine whether VAMP4 plays a functional role in maintaining the structure of the Golgi apparatus, we depleted VAMP4 gene expression using RNA interference technology. Depletion of VAMP4 from HeLa cells led to fragmentation of the Golgi ribbon. These fragments were not uniformly distributed throughout the cytoplasm, but remained in the juxtanuclear area. Electron microscopy and immunohistochemistry showed that in the absence of VAMP4, the length of the Golgi stack was shortened, but Golgi stacking was normal. Anterograde trafficking was not impaired in VAMP4-depleted cells, which contained intact microtubule arrays. Depletion of the cognate SNARE partners of VAMP4, syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure. Our findings suggested that the maintenance of Golgi ribbon structure requires normal retrograde trafficking from the early endosome to the TGN, which is likely to be mediated by the formation of VAMP4-containing SNARE complexes.
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spelling pubmed-36956662013-07-18 VAMP4 is required to maintain the ribbon structure of the Golgi apparatus Shitara, Akiko Shibui, Toru Okayama, Miki Arakawa, Toshiya Mizoguchi, Itaru Shakakura, Yasunori Takuma, Taishin Mol Cell Biochem Article The Golgi apparatus forms a twisted ribbon-like network in the juxtanuclear region of vertebrate cells. Vesicle-associated membrane protein 4 (VAMP4), a v-SNARE protein expressed exclusively in the vertebrate trans-Golgi network (TGN), plays a role in retrograde trafficking from the early endosome to the TGN, although its precise function within the Golgi apparatus remains unclear. To determine whether VAMP4 plays a functional role in maintaining the structure of the Golgi apparatus, we depleted VAMP4 gene expression using RNA interference technology. Depletion of VAMP4 from HeLa cells led to fragmentation of the Golgi ribbon. These fragments were not uniformly distributed throughout the cytoplasm, but remained in the juxtanuclear area. Electron microscopy and immunohistochemistry showed that in the absence of VAMP4, the length of the Golgi stack was shortened, but Golgi stacking was normal. Anterograde trafficking was not impaired in VAMP4-depleted cells, which contained intact microtubule arrays. Depletion of the cognate SNARE partners of VAMP4, syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure. Our findings suggested that the maintenance of Golgi ribbon structure requires normal retrograde trafficking from the early endosome to the TGN, which is likely to be mediated by the formation of VAMP4-containing SNARE complexes. Springer US 2013-05-16 2013 /pmc/articles/PMC3695666/ /pubmed/23677696 http://dx.doi.org/10.1007/s11010-013-1652-4 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Shitara, Akiko
Shibui, Toru
Okayama, Miki
Arakawa, Toshiya
Mizoguchi, Itaru
Shakakura, Yasunori
Takuma, Taishin
VAMP4 is required to maintain the ribbon structure of the Golgi apparatus
title VAMP4 is required to maintain the ribbon structure of the Golgi apparatus
title_full VAMP4 is required to maintain the ribbon structure of the Golgi apparatus
title_fullStr VAMP4 is required to maintain the ribbon structure of the Golgi apparatus
title_full_unstemmed VAMP4 is required to maintain the ribbon structure of the Golgi apparatus
title_short VAMP4 is required to maintain the ribbon structure of the Golgi apparatus
title_sort vamp4 is required to maintain the ribbon structure of the golgi apparatus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695666/
https://www.ncbi.nlm.nih.gov/pubmed/23677696
http://dx.doi.org/10.1007/s11010-013-1652-4
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