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VAMP4 is required to maintain the ribbon structure of the Golgi apparatus
The Golgi apparatus forms a twisted ribbon-like network in the juxtanuclear region of vertebrate cells. Vesicle-associated membrane protein 4 (VAMP4), a v-SNARE protein expressed exclusively in the vertebrate trans-Golgi network (TGN), plays a role in retrograde trafficking from the early endosome t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695666/ https://www.ncbi.nlm.nih.gov/pubmed/23677696 http://dx.doi.org/10.1007/s11010-013-1652-4 |
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author | Shitara, Akiko Shibui, Toru Okayama, Miki Arakawa, Toshiya Mizoguchi, Itaru Shakakura, Yasunori Takuma, Taishin |
author_facet | Shitara, Akiko Shibui, Toru Okayama, Miki Arakawa, Toshiya Mizoguchi, Itaru Shakakura, Yasunori Takuma, Taishin |
author_sort | Shitara, Akiko |
collection | PubMed |
description | The Golgi apparatus forms a twisted ribbon-like network in the juxtanuclear region of vertebrate cells. Vesicle-associated membrane protein 4 (VAMP4), a v-SNARE protein expressed exclusively in the vertebrate trans-Golgi network (TGN), plays a role in retrograde trafficking from the early endosome to the TGN, although its precise function within the Golgi apparatus remains unclear. To determine whether VAMP4 plays a functional role in maintaining the structure of the Golgi apparatus, we depleted VAMP4 gene expression using RNA interference technology. Depletion of VAMP4 from HeLa cells led to fragmentation of the Golgi ribbon. These fragments were not uniformly distributed throughout the cytoplasm, but remained in the juxtanuclear area. Electron microscopy and immunohistochemistry showed that in the absence of VAMP4, the length of the Golgi stack was shortened, but Golgi stacking was normal. Anterograde trafficking was not impaired in VAMP4-depleted cells, which contained intact microtubule arrays. Depletion of the cognate SNARE partners of VAMP4, syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure. Our findings suggested that the maintenance of Golgi ribbon structure requires normal retrograde trafficking from the early endosome to the TGN, which is likely to be mediated by the formation of VAMP4-containing SNARE complexes. |
format | Online Article Text |
id | pubmed-3695666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-36956662013-07-18 VAMP4 is required to maintain the ribbon structure of the Golgi apparatus Shitara, Akiko Shibui, Toru Okayama, Miki Arakawa, Toshiya Mizoguchi, Itaru Shakakura, Yasunori Takuma, Taishin Mol Cell Biochem Article The Golgi apparatus forms a twisted ribbon-like network in the juxtanuclear region of vertebrate cells. Vesicle-associated membrane protein 4 (VAMP4), a v-SNARE protein expressed exclusively in the vertebrate trans-Golgi network (TGN), plays a role in retrograde trafficking from the early endosome to the TGN, although its precise function within the Golgi apparatus remains unclear. To determine whether VAMP4 plays a functional role in maintaining the structure of the Golgi apparatus, we depleted VAMP4 gene expression using RNA interference technology. Depletion of VAMP4 from HeLa cells led to fragmentation of the Golgi ribbon. These fragments were not uniformly distributed throughout the cytoplasm, but remained in the juxtanuclear area. Electron microscopy and immunohistochemistry showed that in the absence of VAMP4, the length of the Golgi stack was shortened, but Golgi stacking was normal. Anterograde trafficking was not impaired in VAMP4-depleted cells, which contained intact microtubule arrays. Depletion of the cognate SNARE partners of VAMP4, syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure. Our findings suggested that the maintenance of Golgi ribbon structure requires normal retrograde trafficking from the early endosome to the TGN, which is likely to be mediated by the formation of VAMP4-containing SNARE complexes. Springer US 2013-05-16 2013 /pmc/articles/PMC3695666/ /pubmed/23677696 http://dx.doi.org/10.1007/s11010-013-1652-4 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Article Shitara, Akiko Shibui, Toru Okayama, Miki Arakawa, Toshiya Mizoguchi, Itaru Shakakura, Yasunori Takuma, Taishin VAMP4 is required to maintain the ribbon structure of the Golgi apparatus |
title | VAMP4 is required to maintain the ribbon structure of the Golgi apparatus |
title_full | VAMP4 is required to maintain the ribbon structure of the Golgi apparatus |
title_fullStr | VAMP4 is required to maintain the ribbon structure of the Golgi apparatus |
title_full_unstemmed | VAMP4 is required to maintain the ribbon structure of the Golgi apparatus |
title_short | VAMP4 is required to maintain the ribbon structure of the Golgi apparatus |
title_sort | vamp4 is required to maintain the ribbon structure of the golgi apparatus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695666/ https://www.ncbi.nlm.nih.gov/pubmed/23677696 http://dx.doi.org/10.1007/s11010-013-1652-4 |
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