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A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors

BACKGROUND: Cansiliella servadeii (Coleoptera) is an endemic troglobite living in deep carbonate caves in North-Eastern Italy. The beetle constantly moves and browses in its preferred habitat (consisting in flowing water and moonmilk, a soft speleothem colonized by microorganisms) self-preens to con...

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Autores principales: Paoletti, Maurizio G, Mazzon, Luca, Martinez-Sañudo, Isabel, Simonato, Mauro, Beggio, Mattia, Dreon, Angelo Leandro, Pamio, Alberto, Brilli, Mauro, Dorigo, Luca, Summers Engel, Annette, Tondello, Alessandra, Baldan, Barbara, Concheri, Giuseppe, Squartini, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695770/
https://www.ncbi.nlm.nih.gov/pubmed/23758657
http://dx.doi.org/10.1186/1471-2180-13-129
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author Paoletti, Maurizio G
Mazzon, Luca
Martinez-Sañudo, Isabel
Simonato, Mauro
Beggio, Mattia
Dreon, Angelo Leandro
Pamio, Alberto
Brilli, Mauro
Dorigo, Luca
Summers Engel, Annette
Tondello, Alessandra
Baldan, Barbara
Concheri, Giuseppe
Squartini, Andrea
author_facet Paoletti, Maurizio G
Mazzon, Luca
Martinez-Sañudo, Isabel
Simonato, Mauro
Beggio, Mattia
Dreon, Angelo Leandro
Pamio, Alberto
Brilli, Mauro
Dorigo, Luca
Summers Engel, Annette
Tondello, Alessandra
Baldan, Barbara
Concheri, Giuseppe
Squartini, Andrea
author_sort Paoletti, Maurizio G
collection PubMed
description BACKGROUND: Cansiliella servadeii (Coleoptera) is an endemic troglobite living in deep carbonate caves in North-Eastern Italy. The beetle constantly moves and browses in its preferred habitat (consisting in flowing water and moonmilk, a soft speleothem colonized by microorganisms) self-preens to convey material from elytra, legs, and antennae towards the mouth. We investigated its inner and outer microbiota using microscopy and DNA-based approaches. RESULTS: Abundant microbial cell masses were observed on the external appendages. Cansiliella’s midgut is fully colonized by live microbes and culture-independent analyses yielded nearly 30 different 16S phylotypes that have no overlap with the community composition of the moonmilk. Many of the lineages, dominated by Gram positive groups, share very low similarity to database sequences. However for most cases, notwithstanding their very limited relatedness with existing records, phylotypes could be assigned to bacterial clades that had been retrieved from insect or other animals’ digestive traits. CONCLUSIONS: Results suggest a history of remote separation from a common ancestor that harboured a set of gut-specific bacteria whose functions are supposedly critical for host physiology. The phylogenetic and coevolutionary implications of the parallel occurrences of these prokaryotic guilds appear to apply throughout a broad spectrum of animal diversity. Their persistence and conservation underlies a possibly critical role of precise bacterial assemblages in animal-bacteria interactions.
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spelling pubmed-36957702013-06-29 A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors Paoletti, Maurizio G Mazzon, Luca Martinez-Sañudo, Isabel Simonato, Mauro Beggio, Mattia Dreon, Angelo Leandro Pamio, Alberto Brilli, Mauro Dorigo, Luca Summers Engel, Annette Tondello, Alessandra Baldan, Barbara Concheri, Giuseppe Squartini, Andrea BMC Microbiol Research Article BACKGROUND: Cansiliella servadeii (Coleoptera) is an endemic troglobite living in deep carbonate caves in North-Eastern Italy. The beetle constantly moves and browses in its preferred habitat (consisting in flowing water and moonmilk, a soft speleothem colonized by microorganisms) self-preens to convey material from elytra, legs, and antennae towards the mouth. We investigated its inner and outer microbiota using microscopy and DNA-based approaches. RESULTS: Abundant microbial cell masses were observed on the external appendages. Cansiliella’s midgut is fully colonized by live microbes and culture-independent analyses yielded nearly 30 different 16S phylotypes that have no overlap with the community composition of the moonmilk. Many of the lineages, dominated by Gram positive groups, share very low similarity to database sequences. However for most cases, notwithstanding their very limited relatedness with existing records, phylotypes could be assigned to bacterial clades that had been retrieved from insect or other animals’ digestive traits. CONCLUSIONS: Results suggest a history of remote separation from a common ancestor that harboured a set of gut-specific bacteria whose functions are supposedly critical for host physiology. The phylogenetic and coevolutionary implications of the parallel occurrences of these prokaryotic guilds appear to apply throughout a broad spectrum of animal diversity. Their persistence and conservation underlies a possibly critical role of precise bacterial assemblages in animal-bacteria interactions. BioMed Central 2013-06-10 /pmc/articles/PMC3695770/ /pubmed/23758657 http://dx.doi.org/10.1186/1471-2180-13-129 Text en Copyright © 2013 Paoletti et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Paoletti, Maurizio G
Mazzon, Luca
Martinez-Sañudo, Isabel
Simonato, Mauro
Beggio, Mattia
Dreon, Angelo Leandro
Pamio, Alberto
Brilli, Mauro
Dorigo, Luca
Summers Engel, Annette
Tondello, Alessandra
Baldan, Barbara
Concheri, Giuseppe
Squartini, Andrea
A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors
title A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors
title_full A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors
title_fullStr A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors
title_full_unstemmed A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors
title_short A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors
title_sort unique midgut-associated bacterial community hosted by the cave beetle cansiliella servadeii (coleoptera: leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695770/
https://www.ncbi.nlm.nih.gov/pubmed/23758657
http://dx.doi.org/10.1186/1471-2180-13-129
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