Cargando…
A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors
BACKGROUND: Cansiliella servadeii (Coleoptera) is an endemic troglobite living in deep carbonate caves in North-Eastern Italy. The beetle constantly moves and browses in its preferred habitat (consisting in flowing water and moonmilk, a soft speleothem colonized by microorganisms) self-preens to con...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695770/ https://www.ncbi.nlm.nih.gov/pubmed/23758657 http://dx.doi.org/10.1186/1471-2180-13-129 |
_version_ | 1782275004670410752 |
---|---|
author | Paoletti, Maurizio G Mazzon, Luca Martinez-Sañudo, Isabel Simonato, Mauro Beggio, Mattia Dreon, Angelo Leandro Pamio, Alberto Brilli, Mauro Dorigo, Luca Summers Engel, Annette Tondello, Alessandra Baldan, Barbara Concheri, Giuseppe Squartini, Andrea |
author_facet | Paoletti, Maurizio G Mazzon, Luca Martinez-Sañudo, Isabel Simonato, Mauro Beggio, Mattia Dreon, Angelo Leandro Pamio, Alberto Brilli, Mauro Dorigo, Luca Summers Engel, Annette Tondello, Alessandra Baldan, Barbara Concheri, Giuseppe Squartini, Andrea |
author_sort | Paoletti, Maurizio G |
collection | PubMed |
description | BACKGROUND: Cansiliella servadeii (Coleoptera) is an endemic troglobite living in deep carbonate caves in North-Eastern Italy. The beetle constantly moves and browses in its preferred habitat (consisting in flowing water and moonmilk, a soft speleothem colonized by microorganisms) self-preens to convey material from elytra, legs, and antennae towards the mouth. We investigated its inner and outer microbiota using microscopy and DNA-based approaches. RESULTS: Abundant microbial cell masses were observed on the external appendages. Cansiliella’s midgut is fully colonized by live microbes and culture-independent analyses yielded nearly 30 different 16S phylotypes that have no overlap with the community composition of the moonmilk. Many of the lineages, dominated by Gram positive groups, share very low similarity to database sequences. However for most cases, notwithstanding their very limited relatedness with existing records, phylotypes could be assigned to bacterial clades that had been retrieved from insect or other animals’ digestive traits. CONCLUSIONS: Results suggest a history of remote separation from a common ancestor that harboured a set of gut-specific bacteria whose functions are supposedly critical for host physiology. The phylogenetic and coevolutionary implications of the parallel occurrences of these prokaryotic guilds appear to apply throughout a broad spectrum of animal diversity. Their persistence and conservation underlies a possibly critical role of precise bacterial assemblages in animal-bacteria interactions. |
format | Online Article Text |
id | pubmed-3695770 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-36957702013-06-29 A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors Paoletti, Maurizio G Mazzon, Luca Martinez-Sañudo, Isabel Simonato, Mauro Beggio, Mattia Dreon, Angelo Leandro Pamio, Alberto Brilli, Mauro Dorigo, Luca Summers Engel, Annette Tondello, Alessandra Baldan, Barbara Concheri, Giuseppe Squartini, Andrea BMC Microbiol Research Article BACKGROUND: Cansiliella servadeii (Coleoptera) is an endemic troglobite living in deep carbonate caves in North-Eastern Italy. The beetle constantly moves and browses in its preferred habitat (consisting in flowing water and moonmilk, a soft speleothem colonized by microorganisms) self-preens to convey material from elytra, legs, and antennae towards the mouth. We investigated its inner and outer microbiota using microscopy and DNA-based approaches. RESULTS: Abundant microbial cell masses were observed on the external appendages. Cansiliella’s midgut is fully colonized by live microbes and culture-independent analyses yielded nearly 30 different 16S phylotypes that have no overlap with the community composition of the moonmilk. Many of the lineages, dominated by Gram positive groups, share very low similarity to database sequences. However for most cases, notwithstanding their very limited relatedness with existing records, phylotypes could be assigned to bacterial clades that had been retrieved from insect or other animals’ digestive traits. CONCLUSIONS: Results suggest a history of remote separation from a common ancestor that harboured a set of gut-specific bacteria whose functions are supposedly critical for host physiology. The phylogenetic and coevolutionary implications of the parallel occurrences of these prokaryotic guilds appear to apply throughout a broad spectrum of animal diversity. Their persistence and conservation underlies a possibly critical role of precise bacterial assemblages in animal-bacteria interactions. BioMed Central 2013-06-10 /pmc/articles/PMC3695770/ /pubmed/23758657 http://dx.doi.org/10.1186/1471-2180-13-129 Text en Copyright © 2013 Paoletti et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Paoletti, Maurizio G Mazzon, Luca Martinez-Sañudo, Isabel Simonato, Mauro Beggio, Mattia Dreon, Angelo Leandro Pamio, Alberto Brilli, Mauro Dorigo, Luca Summers Engel, Annette Tondello, Alessandra Baldan, Barbara Concheri, Giuseppe Squartini, Andrea A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors |
title | A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors |
title_full | A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors |
title_fullStr | A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors |
title_full_unstemmed | A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors |
title_short | A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii (Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors |
title_sort | unique midgut-associated bacterial community hosted by the cave beetle cansiliella servadeii (coleoptera: leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695770/ https://www.ncbi.nlm.nih.gov/pubmed/23758657 http://dx.doi.org/10.1186/1471-2180-13-129 |
work_keys_str_mv | AT paolettimauriziog auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT mazzonluca auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT martinezsanudoisabel auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT simonatomauro auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT beggiomattia auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT dreonangeloleandro auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT pamioalberto auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT brillimauro auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT dorigoluca auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT summersengelannette auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT tondelloalessandra auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT baldanbarbara auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT concherigiuseppe auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT squartiniandrea auniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT paolettimauriziog uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT mazzonluca uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT martinezsanudoisabel uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT simonatomauro uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT beggiomattia uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT dreonangeloleandro uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT pamioalberto uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT brillimauro uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT dorigoluca uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT summersengelannette uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT tondelloalessandra uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT baldanbarbara uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT concherigiuseppe uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors AT squartiniandrea uniquemidgutassociatedbacterialcommunityhostedbythecavebeetlecansiliellaservadeiicoleopteraleptodirinirevealsparallelphylogeneticdivergencesfromuniversalgutspecificancestors |