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Nuclear proteome response to cell wall removal in rice (Oryza sativa)

Plant cells are routinely exposed to various pathogens and environmental stresses that cause cell wall perturbations. Little is known of the mechanisms that plant cells use to sense these disturbances and transduce corresponding signals to regulate cellular responses to maintain cell wall integrity....

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Autores principales: Mujahid, Hana, Tan, Feng, Zhang, Jian, Nallamilli, Babi Ramesh Reddy, Pendarvis, Ken, Peng, Zhaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695858/
https://www.ncbi.nlm.nih.gov/pubmed/23777608
http://dx.doi.org/10.1186/1477-5956-11-26
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author Mujahid, Hana
Tan, Feng
Zhang, Jian
Nallamilli, Babi Ramesh Reddy
Pendarvis, Ken
Peng, Zhaohua
author_facet Mujahid, Hana
Tan, Feng
Zhang, Jian
Nallamilli, Babi Ramesh Reddy
Pendarvis, Ken
Peng, Zhaohua
author_sort Mujahid, Hana
collection PubMed
description Plant cells are routinely exposed to various pathogens and environmental stresses that cause cell wall perturbations. Little is known of the mechanisms that plant cells use to sense these disturbances and transduce corresponding signals to regulate cellular responses to maintain cell wall integrity. Previous studies in rice have shown that removal of the cell wall leads to substantial chromatin reorganization and histone modification changes concomitant with cell wall re-synthesis. But the genes and proteins that regulate these cellular responses are still largely unknown. Here we present an examination of the nuclear proteome differential expression in response to removal of the cell wall in rice suspension cells using multiple nuclear proteome extraction methods. A total of 382 nuclear proteins were identified with two or more peptides, including 26 transcription factors. Upon removal of the cell wall, 142 nuclear proteins were up regulated and 112 were down regulated. The differentially expressed proteins included transcription factors, histones, histone domain containing proteins, and histone modification enzymes. Gene ontology analysis of the differentially expressed proteins indicates that chromatin & nucleosome assembly, protein-DNA complex assembly, and DNA packaging are tightly associated with cell wall removal. Our results indicate that removal of the cell wall imposes a tremendous challenge to the cells. Consequently, plant cells respond to the removal of the cell wall in the nucleus at every level of the regulatory hierarchy.
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spelling pubmed-36958582013-06-29 Nuclear proteome response to cell wall removal in rice (Oryza sativa) Mujahid, Hana Tan, Feng Zhang, Jian Nallamilli, Babi Ramesh Reddy Pendarvis, Ken Peng, Zhaohua Proteome Sci Research Plant cells are routinely exposed to various pathogens and environmental stresses that cause cell wall perturbations. Little is known of the mechanisms that plant cells use to sense these disturbances and transduce corresponding signals to regulate cellular responses to maintain cell wall integrity. Previous studies in rice have shown that removal of the cell wall leads to substantial chromatin reorganization and histone modification changes concomitant with cell wall re-synthesis. But the genes and proteins that regulate these cellular responses are still largely unknown. Here we present an examination of the nuclear proteome differential expression in response to removal of the cell wall in rice suspension cells using multiple nuclear proteome extraction methods. A total of 382 nuclear proteins were identified with two or more peptides, including 26 transcription factors. Upon removal of the cell wall, 142 nuclear proteins were up regulated and 112 were down regulated. The differentially expressed proteins included transcription factors, histones, histone domain containing proteins, and histone modification enzymes. Gene ontology analysis of the differentially expressed proteins indicates that chromatin & nucleosome assembly, protein-DNA complex assembly, and DNA packaging are tightly associated with cell wall removal. Our results indicate that removal of the cell wall imposes a tremendous challenge to the cells. Consequently, plant cells respond to the removal of the cell wall in the nucleus at every level of the regulatory hierarchy. BioMed Central 2013-06-19 /pmc/articles/PMC3695858/ /pubmed/23777608 http://dx.doi.org/10.1186/1477-5956-11-26 Text en Copyright © 2013 Mujahid et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Mujahid, Hana
Tan, Feng
Zhang, Jian
Nallamilli, Babi Ramesh Reddy
Pendarvis, Ken
Peng, Zhaohua
Nuclear proteome response to cell wall removal in rice (Oryza sativa)
title Nuclear proteome response to cell wall removal in rice (Oryza sativa)
title_full Nuclear proteome response to cell wall removal in rice (Oryza sativa)
title_fullStr Nuclear proteome response to cell wall removal in rice (Oryza sativa)
title_full_unstemmed Nuclear proteome response to cell wall removal in rice (Oryza sativa)
title_short Nuclear proteome response to cell wall removal in rice (Oryza sativa)
title_sort nuclear proteome response to cell wall removal in rice (oryza sativa)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695858/
https://www.ncbi.nlm.nih.gov/pubmed/23777608
http://dx.doi.org/10.1186/1477-5956-11-26
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