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A Role for SPARC in the Moderation of Human Insulin Secretion

AIMS/HYPOTHESIS: We have previously shown the implication of the multifunctional protein SPARC (Secreted protein acidic and rich in cysteine)/osteonectin in insulin resistance but potential effects on beta-cell function have not been assessed. We therefore aimed to characterise the effect of SPARC o...

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Autores principales: Harries, Lorna W., McCulloch, Laura J., Holley, Janet E., Rawling, Thomas J., Welters, Hannah J., Kos, Katarina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695891/
https://www.ncbi.nlm.nih.gov/pubmed/23840838
http://dx.doi.org/10.1371/journal.pone.0068253
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author Harries, Lorna W.
McCulloch, Laura J.
Holley, Janet E.
Rawling, Thomas J.
Welters, Hannah J.
Kos, Katarina
author_facet Harries, Lorna W.
McCulloch, Laura J.
Holley, Janet E.
Rawling, Thomas J.
Welters, Hannah J.
Kos, Katarina
author_sort Harries, Lorna W.
collection PubMed
description AIMS/HYPOTHESIS: We have previously shown the implication of the multifunctional protein SPARC (Secreted protein acidic and rich in cysteine)/osteonectin in insulin resistance but potential effects on beta-cell function have not been assessed. We therefore aimed to characterise the effect of SPARC on beta-cell function and features of diabetes. METHODS: We measured SPARC expression by qRT-PCR in human primary pancreatic islets, adipose tissue, liver and muscle. We then examined the relation of SPARC with glucose stimulated insulin secretion (GSIS) in primary human islets and the effect of SPARC overexpression on GSIS in beta cell lines. RESULTS: SPARC was expressed at measurable levels in human islets, adipose tissue, liver and skeletal muscle, and demonstrated reduced expression in primary islets from subjects with diabetes compared with controls (p< = 0.05). SPARC levels were positively correlated with GSIS in islets from control donors (p< = 0.01). Overexpression of SPARC in cultured beta-cells resulted in a 2.4-fold increase in insulin secretion in high glucose conditions (p< = 0.01). CONCLUSIONS: Our data suggest that levels of SPARC are reduced in islets from donors with diabetes and that it has a role in insulin secretion, an effect which appears independent of SPARC’s modulation of obesity-induced insulin resistance in adipose tissue.
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spelling pubmed-36958912013-07-09 A Role for SPARC in the Moderation of Human Insulin Secretion Harries, Lorna W. McCulloch, Laura J. Holley, Janet E. Rawling, Thomas J. Welters, Hannah J. Kos, Katarina PLoS One Research Article AIMS/HYPOTHESIS: We have previously shown the implication of the multifunctional protein SPARC (Secreted protein acidic and rich in cysteine)/osteonectin in insulin resistance but potential effects on beta-cell function have not been assessed. We therefore aimed to characterise the effect of SPARC on beta-cell function and features of diabetes. METHODS: We measured SPARC expression by qRT-PCR in human primary pancreatic islets, adipose tissue, liver and muscle. We then examined the relation of SPARC with glucose stimulated insulin secretion (GSIS) in primary human islets and the effect of SPARC overexpression on GSIS in beta cell lines. RESULTS: SPARC was expressed at measurable levels in human islets, adipose tissue, liver and skeletal muscle, and demonstrated reduced expression in primary islets from subjects with diabetes compared with controls (p< = 0.05). SPARC levels were positively correlated with GSIS in islets from control donors (p< = 0.01). Overexpression of SPARC in cultured beta-cells resulted in a 2.4-fold increase in insulin secretion in high glucose conditions (p< = 0.01). CONCLUSIONS: Our data suggest that levels of SPARC are reduced in islets from donors with diabetes and that it has a role in insulin secretion, an effect which appears independent of SPARC’s modulation of obesity-induced insulin resistance in adipose tissue. Public Library of Science 2013-06-28 /pmc/articles/PMC3695891/ /pubmed/23840838 http://dx.doi.org/10.1371/journal.pone.0068253 Text en © 2013 Harries et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Harries, Lorna W.
McCulloch, Laura J.
Holley, Janet E.
Rawling, Thomas J.
Welters, Hannah J.
Kos, Katarina
A Role for SPARC in the Moderation of Human Insulin Secretion
title A Role for SPARC in the Moderation of Human Insulin Secretion
title_full A Role for SPARC in the Moderation of Human Insulin Secretion
title_fullStr A Role for SPARC in the Moderation of Human Insulin Secretion
title_full_unstemmed A Role for SPARC in the Moderation of Human Insulin Secretion
title_short A Role for SPARC in the Moderation of Human Insulin Secretion
title_sort role for sparc in the moderation of human insulin secretion
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695891/
https://www.ncbi.nlm.nih.gov/pubmed/23840838
http://dx.doi.org/10.1371/journal.pone.0068253
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