Cargando…
Comparative Proteomics-Based Identification of Genes Associated with Glycopeptide Resistance in Clinically Derived Heterogeneous Vancomycin-Intermediate Staphylococcus aureus Strains
Heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) is associated with clinical treatment failure. However, the resistance mechanism of hVISA has not been fully clarified. In the present study, comparative proteomics analysis of two pairs of isogenic vancomycin-susceptible S. aureus...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696005/ https://www.ncbi.nlm.nih.gov/pubmed/23840544 http://dx.doi.org/10.1371/journal.pone.0066880 |
_version_ | 1782476273072734208 |
---|---|
author | Chen, Hongbin Liu, Yali Zhao, Chunjiang Xiao, Di Zhang, Jianzhong Zhang, Feifei Chen, Minjun Wang, Hui |
author_facet | Chen, Hongbin Liu, Yali Zhao, Chunjiang Xiao, Di Zhang, Jianzhong Zhang, Feifei Chen, Minjun Wang, Hui |
author_sort | Chen, Hongbin |
collection | PubMed |
description | Heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) is associated with clinical treatment failure. However, the resistance mechanism of hVISA has not been fully clarified. In the present study, comparative proteomics analysis of two pairs of isogenic vancomycin-susceptible S. aureus (VSSA) and hVISA strains isolated from two patients identified five differentially expressed proteins, IsaA, MsrA2, Asp23, GpmA, and AhpC, present in both isolate pairs. All the proteins were up-regulated in the hVISA strains. These proteins were analyzed in six pairs of isogenic VSSA and hVISA strains, and unrelated VSSA (n = 30) and hVISA (n = 24) by real-time quantitative reverse transcriptase–PCR (qRT–PCR). Of the six pairs of isogenic strains, isaA, msrA2 and ahpC were up-regulated in all six hVISA strains; whereas asp23 and gpmA were up-regulated in five hVISA strains compared with the VSSA parental strains. In the unrelated strains, statistical analyses showed that only isaA was significantly up-regulated in the hVISA strains. Analysis of the five differentially expressed proteins in 15 pairs of persistent VSSA strains by qRT–PCR showed no differences in the expression of the five genes among the persistent strains, suggesting that these genes are not associated with persistence infection. Our results indicate that increased expression of isaA may be related to hVISA resistance. |
format | Online Article Text |
id | pubmed-3696005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36960052013-07-09 Comparative Proteomics-Based Identification of Genes Associated with Glycopeptide Resistance in Clinically Derived Heterogeneous Vancomycin-Intermediate Staphylococcus aureus Strains Chen, Hongbin Liu, Yali Zhao, Chunjiang Xiao, Di Zhang, Jianzhong Zhang, Feifei Chen, Minjun Wang, Hui PLoS One Research Article Heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) is associated with clinical treatment failure. However, the resistance mechanism of hVISA has not been fully clarified. In the present study, comparative proteomics analysis of two pairs of isogenic vancomycin-susceptible S. aureus (VSSA) and hVISA strains isolated from two patients identified five differentially expressed proteins, IsaA, MsrA2, Asp23, GpmA, and AhpC, present in both isolate pairs. All the proteins were up-regulated in the hVISA strains. These proteins were analyzed in six pairs of isogenic VSSA and hVISA strains, and unrelated VSSA (n = 30) and hVISA (n = 24) by real-time quantitative reverse transcriptase–PCR (qRT–PCR). Of the six pairs of isogenic strains, isaA, msrA2 and ahpC were up-regulated in all six hVISA strains; whereas asp23 and gpmA were up-regulated in five hVISA strains compared with the VSSA parental strains. In the unrelated strains, statistical analyses showed that only isaA was significantly up-regulated in the hVISA strains. Analysis of the five differentially expressed proteins in 15 pairs of persistent VSSA strains by qRT–PCR showed no differences in the expression of the five genes among the persistent strains, suggesting that these genes are not associated with persistence infection. Our results indicate that increased expression of isaA may be related to hVISA resistance. Public Library of Science 2013-06-28 /pmc/articles/PMC3696005/ /pubmed/23840544 http://dx.doi.org/10.1371/journal.pone.0066880 Text en © 2013 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chen, Hongbin Liu, Yali Zhao, Chunjiang Xiao, Di Zhang, Jianzhong Zhang, Feifei Chen, Minjun Wang, Hui Comparative Proteomics-Based Identification of Genes Associated with Glycopeptide Resistance in Clinically Derived Heterogeneous Vancomycin-Intermediate Staphylococcus aureus Strains |
title | Comparative Proteomics-Based Identification of Genes Associated with Glycopeptide Resistance in Clinically Derived Heterogeneous Vancomycin-Intermediate Staphylococcus aureus Strains |
title_full | Comparative Proteomics-Based Identification of Genes Associated with Glycopeptide Resistance in Clinically Derived Heterogeneous Vancomycin-Intermediate Staphylococcus aureus Strains |
title_fullStr | Comparative Proteomics-Based Identification of Genes Associated with Glycopeptide Resistance in Clinically Derived Heterogeneous Vancomycin-Intermediate Staphylococcus aureus Strains |
title_full_unstemmed | Comparative Proteomics-Based Identification of Genes Associated with Glycopeptide Resistance in Clinically Derived Heterogeneous Vancomycin-Intermediate Staphylococcus aureus Strains |
title_short | Comparative Proteomics-Based Identification of Genes Associated with Glycopeptide Resistance in Clinically Derived Heterogeneous Vancomycin-Intermediate Staphylococcus aureus Strains |
title_sort | comparative proteomics-based identification of genes associated with glycopeptide resistance in clinically derived heterogeneous vancomycin-intermediate staphylococcus aureus strains |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696005/ https://www.ncbi.nlm.nih.gov/pubmed/23840544 http://dx.doi.org/10.1371/journal.pone.0066880 |
work_keys_str_mv | AT chenhongbin comparativeproteomicsbasedidentificationofgenesassociatedwithglycopeptideresistanceinclinicallyderivedheterogeneousvancomycinintermediatestaphylococcusaureusstrains AT liuyali comparativeproteomicsbasedidentificationofgenesassociatedwithglycopeptideresistanceinclinicallyderivedheterogeneousvancomycinintermediatestaphylococcusaureusstrains AT zhaochunjiang comparativeproteomicsbasedidentificationofgenesassociatedwithglycopeptideresistanceinclinicallyderivedheterogeneousvancomycinintermediatestaphylococcusaureusstrains AT xiaodi comparativeproteomicsbasedidentificationofgenesassociatedwithglycopeptideresistanceinclinicallyderivedheterogeneousvancomycinintermediatestaphylococcusaureusstrains AT zhangjianzhong comparativeproteomicsbasedidentificationofgenesassociatedwithglycopeptideresistanceinclinicallyderivedheterogeneousvancomycinintermediatestaphylococcusaureusstrains AT zhangfeifei comparativeproteomicsbasedidentificationofgenesassociatedwithglycopeptideresistanceinclinicallyderivedheterogeneousvancomycinintermediatestaphylococcusaureusstrains AT chenminjun comparativeproteomicsbasedidentificationofgenesassociatedwithglycopeptideresistanceinclinicallyderivedheterogeneousvancomycinintermediatestaphylococcusaureusstrains AT wanghui comparativeproteomicsbasedidentificationofgenesassociatedwithglycopeptideresistanceinclinicallyderivedheterogeneousvancomycinintermediatestaphylococcusaureusstrains |