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Unbiased Approach for Virus Detection in Skin Lesions

To assess presence of virus DNA in skin lesions, swab samples from 82 squamous cell carcinomas of the skin (SCCs), 60 actinic keratoses (AKs), paraffin-embedded biopsies from 28 SCCs and 72 kerathoacanthomas (KAs) and fresh-frozen biopsies from 92 KAs, 85 SCCs and 92 AKs were analyzed by high throug...

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Autores principales: Bzhalava, Davit, Johansson, Hanna, Ekström, Johanna, Faust, Helena, Möller, Birgitta, Eklund, Carina, Nordin, Peter, Stenquist, Bo, Paoli, John, Persson, Bengt, Forslund, Ola, Dillner, Joakim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696016/
https://www.ncbi.nlm.nih.gov/pubmed/23840382
http://dx.doi.org/10.1371/journal.pone.0065953
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author Bzhalava, Davit
Johansson, Hanna
Ekström, Johanna
Faust, Helena
Möller, Birgitta
Eklund, Carina
Nordin, Peter
Stenquist, Bo
Paoli, John
Persson, Bengt
Forslund, Ola
Dillner, Joakim
author_facet Bzhalava, Davit
Johansson, Hanna
Ekström, Johanna
Faust, Helena
Möller, Birgitta
Eklund, Carina
Nordin, Peter
Stenquist, Bo
Paoli, John
Persson, Bengt
Forslund, Ola
Dillner, Joakim
author_sort Bzhalava, Davit
collection PubMed
description To assess presence of virus DNA in skin lesions, swab samples from 82 squamous cell carcinomas of the skin (SCCs), 60 actinic keratoses (AKs), paraffin-embedded biopsies from 28 SCCs and 72 kerathoacanthomas (KAs) and fresh-frozen biopsies from 92 KAs, 85 SCCs and 92 AKs were analyzed by high throughput sequencing (HTS) using 454 or Ion Torrent technology. We found total of 4,284 viral reads, out of which 4,168 were Human Papillomavirus (HPV)-related, belonging to 15 known (HPV8, HPV12, HPV20, HPV36, HPV38, HPV45, HPV57, HPV59, HPV104, HPV105, HPV107, HPV109, HPV124, HPV138, HPV147), four previously described putative (HPV 915 F 06 007 FD1, FA73, FA101, SE42) and two putatively new HPV types (SE46, SE47). SE42 was cloned, sequenced, designated as HPV155 and found to have 76% similarity to the most closely related known HPV type. In conclusion, an unbiased approach for viral DNA detection in skin tumors has found that, although some new putative HPVs were found, known HPV types constituted most of the viral DNA.
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spelling pubmed-36960162013-07-09 Unbiased Approach for Virus Detection in Skin Lesions Bzhalava, Davit Johansson, Hanna Ekström, Johanna Faust, Helena Möller, Birgitta Eklund, Carina Nordin, Peter Stenquist, Bo Paoli, John Persson, Bengt Forslund, Ola Dillner, Joakim PLoS One Research Article To assess presence of virus DNA in skin lesions, swab samples from 82 squamous cell carcinomas of the skin (SCCs), 60 actinic keratoses (AKs), paraffin-embedded biopsies from 28 SCCs and 72 kerathoacanthomas (KAs) and fresh-frozen biopsies from 92 KAs, 85 SCCs and 92 AKs were analyzed by high throughput sequencing (HTS) using 454 or Ion Torrent technology. We found total of 4,284 viral reads, out of which 4,168 were Human Papillomavirus (HPV)-related, belonging to 15 known (HPV8, HPV12, HPV20, HPV36, HPV38, HPV45, HPV57, HPV59, HPV104, HPV105, HPV107, HPV109, HPV124, HPV138, HPV147), four previously described putative (HPV 915 F 06 007 FD1, FA73, FA101, SE42) and two putatively new HPV types (SE46, SE47). SE42 was cloned, sequenced, designated as HPV155 and found to have 76% similarity to the most closely related known HPV type. In conclusion, an unbiased approach for viral DNA detection in skin tumors has found that, although some new putative HPVs were found, known HPV types constituted most of the viral DNA. Public Library of Science 2013-06-28 /pmc/articles/PMC3696016/ /pubmed/23840382 http://dx.doi.org/10.1371/journal.pone.0065953 Text en © 2013 Bzhalava et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bzhalava, Davit
Johansson, Hanna
Ekström, Johanna
Faust, Helena
Möller, Birgitta
Eklund, Carina
Nordin, Peter
Stenquist, Bo
Paoli, John
Persson, Bengt
Forslund, Ola
Dillner, Joakim
Unbiased Approach for Virus Detection in Skin Lesions
title Unbiased Approach for Virus Detection in Skin Lesions
title_full Unbiased Approach for Virus Detection in Skin Lesions
title_fullStr Unbiased Approach for Virus Detection in Skin Lesions
title_full_unstemmed Unbiased Approach for Virus Detection in Skin Lesions
title_short Unbiased Approach for Virus Detection in Skin Lesions
title_sort unbiased approach for virus detection in skin lesions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696016/
https://www.ncbi.nlm.nih.gov/pubmed/23840382
http://dx.doi.org/10.1371/journal.pone.0065953
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