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Passive immunization by recombinant ferric enterobactin protein (FepA) from Escherichia coli O157
BACKGROUND AND OBJECTIVES: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 has been recognized as a major food borne pathogen responsible for frequent hemorrhagic colitis and hemolytic uremic syndrome in humans. Cattle are important reservoirs of E. coli O157:H7, in which the organism colonizes th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696845/ https://www.ncbi.nlm.nih.gov/pubmed/23825727 |
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author | Larrie-Bagha, Seyed Mehdi Rasooli, Iraj Mousavi-Gargari, Seyed Latif Rasooli, Zohreh Nazarian, Shahram |
author_facet | Larrie-Bagha, Seyed Mehdi Rasooli, Iraj Mousavi-Gargari, Seyed Latif Rasooli, Zohreh Nazarian, Shahram |
author_sort | Larrie-Bagha, Seyed Mehdi |
collection | PubMed |
description | BACKGROUND AND OBJECTIVES: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 has been recognized as a major food borne pathogen responsible for frequent hemorrhagic colitis and hemolytic uremic syndrome in humans. Cattle are important reservoirs of E. coli O157:H7, in which the organism colonizes the intestinal tract and is shed in the feces. OBJECTIVE: Vaccination of cattle has significant potential as a pre-harvest intervention strategy for E. coli O157:H7. The aim of this study was to evaluate active and passive immunization against E. coli O157:H7 using a recombinant protein. MATERIALS AND METHODS: The recombinant FepA protein induced by IPTG was purified by nickel affinity chromatography. Antibody titre was determined by ELISA in FepA immunized rabbits sera. Sera collected from vaccinated animals were used for bacterial challenge in passive immunization studies. RESULTS: The results demonstrate that passive immunization with serum raised against FepA protects rabbits from subsequent infection. CONCLUSION: Significant recognition by the antibody of ferric enterobactin binding protein may lead to its application in the restriction of Enterobacteriaceae propagation. |
format | Online Article Text |
id | pubmed-3696845 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-36968452013-07-02 Passive immunization by recombinant ferric enterobactin protein (FepA) from Escherichia coli O157 Larrie-Bagha, Seyed Mehdi Rasooli, Iraj Mousavi-Gargari, Seyed Latif Rasooli, Zohreh Nazarian, Shahram Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 has been recognized as a major food borne pathogen responsible for frequent hemorrhagic colitis and hemolytic uremic syndrome in humans. Cattle are important reservoirs of E. coli O157:H7, in which the organism colonizes the intestinal tract and is shed in the feces. OBJECTIVE: Vaccination of cattle has significant potential as a pre-harvest intervention strategy for E. coli O157:H7. The aim of this study was to evaluate active and passive immunization against E. coli O157:H7 using a recombinant protein. MATERIALS AND METHODS: The recombinant FepA protein induced by IPTG was purified by nickel affinity chromatography. Antibody titre was determined by ELISA in FepA immunized rabbits sera. Sera collected from vaccinated animals were used for bacterial challenge in passive immunization studies. RESULTS: The results demonstrate that passive immunization with serum raised against FepA protects rabbits from subsequent infection. CONCLUSION: Significant recognition by the antibody of ferric enterobactin binding protein may lead to its application in the restriction of Enterobacteriaceae propagation. Tehran University of Medical Sciences 2013-06 /pmc/articles/PMC3696845/ /pubmed/23825727 Text en © 2013 Iranian Society of Microbiology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Larrie-Bagha, Seyed Mehdi Rasooli, Iraj Mousavi-Gargari, Seyed Latif Rasooli, Zohreh Nazarian, Shahram Passive immunization by recombinant ferric enterobactin protein (FepA) from Escherichia coli O157 |
title | Passive immunization by recombinant ferric enterobactin protein (FepA) from Escherichia coli O157 |
title_full | Passive immunization by recombinant ferric enterobactin protein (FepA) from Escherichia coli O157 |
title_fullStr | Passive immunization by recombinant ferric enterobactin protein (FepA) from Escherichia coli O157 |
title_full_unstemmed | Passive immunization by recombinant ferric enterobactin protein (FepA) from Escherichia coli O157 |
title_short | Passive immunization by recombinant ferric enterobactin protein (FepA) from Escherichia coli O157 |
title_sort | passive immunization by recombinant ferric enterobactin protein (fepa) from escherichia coli o157 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696845/ https://www.ncbi.nlm.nih.gov/pubmed/23825727 |
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